LOCUS BC104898 5289 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens Rho GTPase-activating protein, mRNA (cDNA clone
MGC:132558 IMAGE:8143901), complete cds.
ACCESSION BC104898
VERSION BC104898.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5289)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5289)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-SEP-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 3 Row: g Column: 3.
FEATURES Location/Qualifiers
source 1..5289
/db_xref="H-InvDB:HIT000337986"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:132558 IMAGE:8143901"
/tissue_type="Colon, PCR rescued clones"
/clone_lib="NIH_MGC_294"
/note="Vector: pCR4-TOPO; Clone identification sequence
tag: CAAAGCTG"
gene 1..5289
/gene="RICS"
/gene_synonym="GC-GAP"
/gene_synonym="GRIT"
/gene_synonym="KIAA0712"
/gene_synonym="MGC1892"
/gene_synonym="p200RhoGAP"
/gene_synonym="p250GAP"
/db_xref="GeneID:9743"
/db_xref="MIM:608541"
CDS 35..5251
/gene="RICS"
/gene_synonym="GC-GAP"
/gene_synonym="GRIT"
/gene_synonym="KIAA0712"
/gene_synonym="MGC1892"
/gene_synonym="p200RhoGAP"
/gene_synonym="p250GAP"
/codon_start=1
/product="Rho GTPase-activating protein"
/protein_id="AAI04899.1"
/db_xref="GeneID:9743"
/db_xref="MIM:608541"
/translation="MKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCT
AFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFR
ELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLAD
YCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLF
SGRISMAMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVNSPIVTENKYIEVG
EGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRNESE
PSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDSKLFRPRRPRSSSDALSASFNG
EMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAEDVDLSPPDIGVASLDFDPMS
FQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGSSQCQTPGSTASSEPVSP
LQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLSKSPSMSISEPISVTL
PPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMKTNETVAQEAYESE
VQPLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQDSVPVSSVSLIP
PPPPPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGDIAVATTEDNLSSSYS
AVALDKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTHSNTTESGEQHHQVDL
TGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKNSMPTVSFLDQDQSPP
RFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPEENTSTATMTY
MTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQRSAEQPPVV
GQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQCPMATAVQ
PGLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESKMIAAIHSSSADATSS
SNYHSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPDVTTEPFGPDNCLHFN
MTPNCQYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAPGRNASGHHSKPCS
RVEYVSSLSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPDDEPA
YCPRPLYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGALCD
VDAYGTVQLRPLHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHNVV
SMPPAADVKHTYTSWDLEDMEKYRMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDLG
GIYVIHLRSKSDPGKTGLLSVAEGKESRHAAKAISPEGEDRFYRRHPEAEMDRAHHHG
GHGSTQPEKPSLPQKQSSLRSRKLPDMGCSLPEHRAHQEASHRQFCESKNGPPYPQGA
GQLDYGSKGIPDTSEPVSYHNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAP
EKHSRDCYKEEEHLTQSIVPPPKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSV
TVVSQYDNLEDYHSLPQHQRGVFGGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAEL
SLQHPETQIHAE"
BASE COUNT 1415 a 1454 c 1224 g 1196 t
ORIGIN
1 gcacggcaag ctcattacgt tcttacgaac attcatgaag tctcgtccaa caaaacagaa
61 gctgaagcag cggggaatct tgaaagagag ggtgtttggt tgtgacctgg gggagcacct
121 tctaaattct ggttttgaag tgccgcaggt tcttcaaagc tgcacagcat tcattgagag
181 atatggcatc gtggatggaa tctatcgcct ttctggtgtt gcctccaata tccagagact
241 acgccatgaa tttgactctg agcacgtccc cgacctgacg aaagaaccgt atgttcagga
301 catccattct gtgggttccc tatgtaagct gtacttccgg gaactcccaa accctctgct
361 tacctaccag ctgtatgaga aattttctga tgcagtttca gcagcaacag atgaagaaag
421 gctgataaaa atccacgatg tcatccagca gctcccccca ccacactaca gaacactgga
481 gttcctgatg agacacttgt ctcttctagc tgactattgt tccatcacaa atatgcatgc
541 aaaaaatcta gcaattgttt gggctccaaa cctgttaaga tcaaaacaga tagaatctgc
601 ctgcttcagt ggaacagcag ctttcatgga agtgaggatt cagtctgtgg ttgttgagtt
661 catcctgaat cacgttgatg tgctgttcag cggcagaatc agcatggcca tgcaagaggg
721 ggcagcttct ctatcaaggc ccaagtccct cctggtatcc tctccatcca ccaaactgct
781 gacattggaa gaggcccagg cacgaacaca agctcaggtc aattctccaa ttgtgacgga
841 aaataaatat atcgaagtag gagaaggacc tgctgcactt caggggaaat ttcataccat
901 aattgagttc ccacttgaaa gaaagaggcc tcaaaataag atgaaaaagt ctcctgtggg
961 tagctggcgt tcctttttca acttggggaa atcatcatct gtttctaaac gaaagctgca
1021 gcggaatgag agtgagcctt cagagatgaa agccatggct ctgaaaggtg gcagggcaga
1081 aggaaccctc cgttcagcta aaagtgagga gtctcttaca tctctccatg cagttgatgg
1141 tgattctaag ctcttccgac ccagaagacc cagatcttcc agtgatgcac tgtctgcctc
1201 ttttaatgga gaaatgctgg ggaaccgctg taactcctat gataatctgc ctcatgacaa
1261 tgagagtgag gaggaaggag ggctgcttca tatcccagcc cttatgtctc ctcattcagc
1321 tgaggatgtt gacttgagcc caccagacat tggagtagcc agcctggatt ttgatccaat
1381 gtcatttcaa tgtagtcctc ctaaggccga atcagaatgt ctggagagtg gtgcttcctt
1441 tttagattca ccaggatact ccaaggataa accaagtgcc aataaaaagg atgcagaaac
1501 aggtagtagc caatgtcaga ctccaggaag cacagcaagc tctgaacctg tctctcctct
1561 tcaggagaaa ctgagtccat tctttaccct ggacttgagc ccaactgaag ataaatcatc
1621 taagccatcc tcctttactg aaaaggtcgt ctatgctttc tctccgaaga taggacggaa
1681 attaagcaaa tcaccttcta tgagcatatc tgagccaatt tcagtgaccc taccaccacg
1741 ggtgtcagaa gtcattggta cagtctcaaa taccacagct cagaatgcat catcttcaac
1801 ctgggacaaa tgcgttgaag aaagggatgc cacaaataga tcccccaccc agatagtaaa
1861 gatgaaaaca aatgagacag ttgcccaaga agcatatgaa tctgaagtcc agcccctgga
1921 ccaggtggct gctgaagaag tagaattgcc agggaaagag gatcagtctg tctcaagcag
1981 tcagagtaag gctgtagctt ctggacagac tcagacagga gcagttaccc atgacccccc
2041 tcaggattcc gttcctgtca gttcagtctc tcttatccca ccaccaccgc ctccgaaaaa
2101 tgttgcccga atgttggcgc tagcattagc tgagtccgca cagcaagcct caactcagtc
2161 attgaagaga ccagggacct ctcaggctgg gtatacaaat tatggagaca tagcggtggc
2221 tacaactgaa gataatctgt ccagttctta ctctgcagtt gctctagata aggcctattt
2281 ccaaaccgat cgaccagcag agcagttcca cctccagaat aatgcaccag gaaactgtga
2341 ccatcctcta ccagagacaa cagctactgg ggatcctacc cattccaaca caactgaatc
2401 tggggagcaa catcaccaag tagacttaac agggaatcag ccacatcaag catatttatc
2461 tggggaccca gaaaaggcca gaattacttc agttccctta gactcagaga agtctgatga
2521 tcatgtaagt ttccctgaag accagtctgg gaagaacagt atgccaactg tctccttctt
2581 ggatcaggac cagtctccac cccgtttcta cagtggagat cagcctcctt cttatcttgg
2641 tgcaagtgtg gataaactcc atcacccttt agaatttgca gacaaatctc ccacacctcc
2701 taatttacct agcgataaaa tctaccctcc ttctgggtcc cccgaagaga ataccagcac
2761 agccaccatg acttacatga caactactcc agcaacagcc caaatgagca ccaaggaagc
2821 cagctgggat gtggctgaac aacccaccac tgctgatttt gctgctgcca cacttcagcg
2881 cacgcacaga actaatcgtc cccttccccc tccgccttcc cagagatctg cagagcagcc
2941 accagttgtg gggcaggtac aagcagcaac caatatagga ttaaataatt cccacaaggt
3001 tcaaggagta gttccagttc cagagaggcc acctgaacct cgagccatgg atgaccctgc
3061 gtctgccttc atcagtgaca gtggtgctgc tgctgctcag tgtcccatgg ctacagctgt
3121 ccagccaggc ctgcctgaga aagtgcggga cggtgcccgg gtcccgctgc tgcacctgcg
3181 cgccgagtct gtccctgcgc atccctgtgg ctttcctgca ccactgcccc ccaccaggat
3241 gatggagagt aagatgattg ctgccataca ctccagcagt gcagatgcca ccagcagttc
3301 aaattatcat tcctttgtca ctgcttcatc cacctctgtg gacgatgcat tgcctttacc
3361 acttcctgtc ccacaaccta agcatgcttc tcagaaaaca gtttactcct cctttgctag
3421 gcccgatgtc accactgaac cctttggtcc agataactgt ttgcatttca atatgactcc
3481 aaactgccag taccgtcccc agagtgtacc tccccatcac aataaattgg agcagcacca
3541 agtgtatggt gccaggtcag agccaccagc ctccatgggt cttcgttata acacatatgt
3601 ggccccagga agaaacgcat ctggacacca ctccaagcca tgcagccggg tcgagtatgt
3661 gtcttctttg agctcctctg tcaggaatac ctgttacccc gaagacattc caccgtaccc
3721 taccatccgg agagtgcagt ctctccatgc tccgccgtct tccatgattc gctctgttcc
3781 catttcacgg acagaagttc ccccagatga tgagccagcc tactgcccaa gacctctgta
3841 ccaatataag ccatatcagt cctcccaggc ccgctcagat tatcatgtca ctcagcttca
3901 gccttacttt gagaatggcc gggtccacta caggtatagc ccatattcca gttcttctag
3961 ttcctattac agtccagatg gggccctgtg tgatgtggat gcctatggca cagtccagtt
4021 gagacccctt caccgccttc ccaatcgaga ctttgctttc tacaatccta ggctgcaagg
4081 aaagagcttg tacagttatg ctggtttggc tccacgtccc cgggccaacg tgactggcta
4141 tttctctccc aacgaccata atgtagtcag catgcctccg gctgctgatg tgaagcacac
4201 ctacacctca tgggatcttg aggacatgga aaaataccgc atgcagtcca tccggagaga
4261 gagccgtgct cggcagaagg tgaaagggcc tgtcatgtcc caatatgata acatgacccc
4321 ggcggtgcag gacgacttgg gtgggatcta tgtcatccat ctgcgtagta aatcagatcc
4381 tgggaaaact ggacttctct cagtggcaga aggaaaggag agccgccatg cagccaaggc
4441 catcagtccc gagggagagg accgcttcta taggaggcat cccgaggcag agatggacag
4501 agcccaccat cacggaggcc atggtagcac gcagccggag aagccatccc tgcctcagaa
4561 gcagagcagc ctgaggagca ggaagcttcc tgacatgggc tgcagtcttc ctgagcacag
4621 ggcacaccaa gaagcaagcc ataggcagtt ctgtgagtca aagaatgggc ccccttatcc
4681 ccagggagct ggccagttag attatgggtc caaagggatt ccagacactt ctgagccagt
4741 cagctaccac aactctggag taaaatatgc tgcatccggg caagaatctt taagactgaa
4801 ccacaaagag gtaaggctct ccaaagagat ggagcgaccc tgggttaggc agccttctgc
4861 cccagagaaa cactccagag actgctacaa ggaggaagaa cacctcactc agtcaatcgt
4921 cccaccccct aaaccagaga ggagtcatag cctcaaactc catcataccc agaacgtgga
4981 gagggacccc agtgtgctgt accagtacca accacacggc aagcgccaga gcagtgtgac
5041 tgttgtgtcc cagtatgata acctggaaga ttaccactcc ctgcctcagc accagcgagg
5101 agtctttgga gggggcggca tggggacgta tgtgccccct ggctttcccc atccacagag
5161 caggacctat gctacagcgt tgggtcaagg ggccttcctg cccgcagagt tgtccttgca
5221 gcatcctgaa acacagatcc atgcagaatg agccctgcga gcaatagagt tgaagcagcc
5281 tctgctgga
//