LOCUS BC101480 2394 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens phosphodiesterase 4B, cAMP-specific (phosphodiesterase
E4 dunce homolog, Drosophila), mRNA (cDNA clone MGC:126529
IMAGE:8068986), complete cds.
ACCESSION BC101480
VERSION BC101480.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2394)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2394)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-AUG-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 2 Row: A Column: 16.
FEATURES Location/Qualifiers
source 1..2394
/db_xref="H-InvDB:HIT000336849"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:126529 IMAGE:8068986"
/tissue_type="Brain, cerebellum, PCR rescued clones"
/clone_lib="NIH_MGC_311"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: CCAAGGCT"
gene 1..2394
/gene="PDE4B"
/gene_synonym="PDEIVB"
/db_xref="GeneID:5142"
/db_xref="HGNC:HGNC:8781"
/db_xref="MIM:600127"
CDS 87..2297
/gene="PDE4B"
/gene_synonym="PDEIVB"
/codon_start=1
/product="phosphodiesterase 4B, cAMP-specific
(phosphodiesterase E4 dunce homolog, Drosophila)"
/protein_id="AAI01481.1"
/db_xref="GeneID:5142"
/db_xref="HGNC:HGNC:8781"
/db_xref="MIM:600127"
/translation="MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLWRGRR
CCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLTTLPSIAITTVSQECFDVENGPS
PGRSPLDPQASSSAGLVLHATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQH
GDDLIVTPFAQVLASLRSVRNNFTILTNLHGTSNKRSPAASQPPVSRVNPQEESYQKL
AMETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYIS
NTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTEN
EDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLKTFRISSDTFITYM
MTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDH
PGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFMNLTKKQRQTLRK
MVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLS
NPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVH
PLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDEQNRDCQGLMEKFQFEL
TLDEEDSEGPEKEGEGHSYFSSTKTLCVIDPENRDSLGETDIDIATEDKSPVDT"
BASE COUNT 720 a 560 c 557 g 557 t
ORIGIN
1 gcgagggcag cctgaggtat taaaaagtgt cagcaaactg cattgaataa cagacatcct
61 aagaggggat attttccacc tctataatga agaaaagcag gagtgtgatg acggtgatgg
121 ctgatgataa tgttaaagat tattttgaat gtagcttgag taaatcctac agttcttcca
181 gtaacacact tgggatcgac ctctggagag ggagaaggtg ttgctcagga aacttacagt
241 taccaccact gtctcaaaga cagagtgaaa gggcaaggac tcctgaggga gatggtattt
301 ccaggccgac cacactgcct ttgacaacgc ttccaagcat tgctattaca actgtaagcc
361 aggagtgctt tgatgtggaa aatggccctt ccccaggtcg gagtccactg gatccccagg
421 ccagctcttc cgctgggctg gtacttcacg ccacctttcc tgggcacagc cagcgcagag
481 agtcatttct ctacagatca gacagcgact atgacttgtc accaaaggcg atgtcgagaa
541 actcttctct tccaagcgag caacacggcg atgacttgat tgtaactcct tttgcccagg
601 tccttgccag cttgcgaagt gtgagaaaca acttcactat actgacaaac cttcatggta
661 catctaacaa gaggtcccca gctgctagtc agcctcctgt ctccagagtc aacccacaag
721 aagaatctta tcaaaaatta gcaatggaaa cgctggagga attagactgg tgtttagacc
781 agctagagac catacagacc taccggtctg tcagtgagat ggcttctaac aagttcaaaa
841 gaatgctgaa ccgggagctg acacacctct cagagatgag ccgatcaggg aaccaggtgt
901 ctgaatacat ttcaaatact ttcttagaca agcagaatga tgtggagatc ccatctccta
961 cccagaaaga cagggagaaa aagaaaaagc agcagctcat gacccagata agtggagtga
1021 agaaattaat gcatagttca agcctaaaca atacaagcat ctcacgcttt ggagtcaaca
1081 ctgaaaatga agatcacctg gccaaggagc tggaagacct gaacaaatgg ggtcttaaca
1141 tctttaatgt ggctggatat tctcacaata gacccctaac atgcatcatg tatgctatat
1201 tccaggaaag agacctccta aagacattca gaatctcatc tgacacattt ataacctaca
1261 tgatgacttt agaagaccat taccattctg acgtggcata tcacaacagc ctgcacgctg
1321 ctgatgtagc ccagtcgacc catgttctcc tttctacacc agcattagac gctgtcttca
1381 cagatttgga gatcctggct gccatttttg cagctgccat ccatgacgtt gatcatcctg
1441 gagtctccaa tcagtttctc atcaacacaa attcagaact tgctttgatg tataatgatg
1501 aatctgtgtt ggaaaatcat caccttgctg tgggtttcaa actgctgcaa gaagaacact
1561 gtgacatctt catgaatctc accaagaagc agcgtcagac actcaggaag atggttattg
1621 acatggtgtt agcaactgat atgtctaaac atatgagcct gctggcagac ctgaagacaa
1681 tggtagaaac gaagaaagtt acaagttcag gcgttcttct cctagacaac tataccgatc
1741 gcattcaggt ccttcgcaac atggtacact gtgcagacct gagcaacccc accaagtcct
1801 tggaattgta tcggcaatgg acagaccgca tcatggagga atttttccag cagggagaca
1861 aagagcggga gaggggaatg gaaattagcc caatgtgtga taaacacaca gcttctgtgg
1921 aaaaatccca ggttggtttc atcgactaca ttgtccatcc attgtgggag acatgggcag
1981 atttggtaca gcctgatgct caggacattc tcgatacctt agaagataac aggaactggt
2041 atcagagcat gatacctcaa agtccctcac caccactgga cgagcagaac agggactgcc
2101 agggtctgat ggagaagttt cagtttgaac tgactctcga tgaggaagat tctgaaggac
2161 ctgagaagga gggagaggga cacagctatt tcagcagcac aaagacgctt tgtgtgattg
2221 atccagaaaa cagagattcc ctgggagaga ctgacataga cattgcaaca gaagacaagt
2281 cccccgtgga tacataatcc ccctctccct gtggagatga acattctatc cttgatgagc
2341 atgccagcta tgtggtaggg ccagcccacc atgggggcca agacctgcac agga
//