LOCUS BC100762 678 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens myosin light chain kinase, mRNA (cDNA clone
IMAGE:40000098), complete cds.
ACCESSION BC100762
VERSION BC100762.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 678)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 678)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-AUG-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 5 Row: b Column: 3
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..678
/db_xref="H-InvDB:HIT000386310"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:40000098"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_282"
/note="Vector: pCR-Blunt II-TOPO; Clone identification
sequence tag: ACCTAATT sequenced from the reverse primer"
gene 1..678
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
CDS 79..543
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/codon_start=1
/product="myosin light chain kinase"
/protein_id="AAI00763.2"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
/translation="MAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEK
PHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDED
GNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE"
BASE COUNT 216 a 136 c 174 g 152 t
ORIGIN
1 caacgcctga gtccaaccct cgtgtctatt ttccagaaaa cgggcaatgc tgtgagagcc
61 attggaagac tgtcctctat ggcaatgatc tcagggctca gtggcaggaa atcctcaaca
121 gggtcaccaa ccagcccgct caatgcagaa aaactagaat ctgaagaaga tgtgtcccaa
181 gctttccttg aggctgttgc tgaggaaaag cctcatgtaa aaccctattt ctctaagacc
241 attcgcgatt tagaagttgt ggagggaagt gctgctagat ttgactgcaa gattgaagga
301 tacccagacc ccgaggttgt ctggttcaaa gatgaccagt caatcaggga gtcccgccac
361 ttccagatag actacgatga ggacgggaac tgctctttaa ttattagtga tgtttgcggg
421 gatgacgatg ccaagtacac ctgcaaggct gtcaacagtc ttggagaagc cacctgcaca
481 gcagagctca ttgtggaaac gatggaggaa ggtgaagggg aaggggaaga ggaagaagag
541 tgaaacaaag ccagagaaaa gcagtttcta agtcatatta aaaggactat ttctctaaaa
601 ctcaaaaaaa aaaaaaaaac tcaagatagt aaaagcacct agtgtgatag attatcggtt
661 aggtcatttg tgggttga
//