LOCUS BC098443 3220 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens cholinergic receptor, nicotinic, alpha 3, mRNA (cDNA
clone MGC:104879 IMAGE:3508233), complete cds.
ACCESSION BC098443
VERSION BC098443.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3220)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3220)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 62 Row: a Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene
prediction.
FEATURES Location/Qualifiers
source 1..3220
/db_xref="H-InvDB:HIT000335997"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:104879 IMAGE:3508233"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3220
/gene="CHRNA3"
/db_xref="GeneID:1136"
/db_xref="HGNC:HGNC:1957"
/db_xref="MIM:118503"
CDS 386..1900
/gene="CHRNA3"
/codon_start=1
/product="cholinergic receptor, nicotinic, alpha 3"
/protein_id="AAH98443.1"
/db_xref="GeneID:1136"
/db_xref="HGNC:HGNC:1957"
/db_xref="MIM:118503"
/translation="MGSGPLSLPLALSPPRLLLLLLSLLPVARASEAEHRLFERLFED
YNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDY
GGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKI
DVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDI
KYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCI
SVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTT
HTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCKE
GYPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKYI
AENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQPLMAREDA"
BASE COUNT 783 a 854 c 759 g 824 t
ORIGIN
1 ccttcctggt ggtggtgaca gcacctgctc agatctggtc ggacgccgcc ggccggagca
61 cccagcccgg cggagaagga gctcgcccgg cgctggggac tgggacctgg agccccttcc
121 cctaccgcac gtacgccccg ccccgcgcac gcccgcccgc ccgcccgcgc ctggcgcagc
181 ttcactccgg atggttcctg tcctcccgcg ggtccgaggg cgctggaaac ccagcggcgg
241 cgaagcggag aggagccccg cgcgtctccg cccgcacggc tccaggtctg gggtctgcgc
301 tggagccgcg cggggagagg ccgtctctgc gaccgccgcg cccgctcccg accgtccggg
361 tccgcggcca gcccggccac cagccatggg ctctggcccg ctctcgctgc ccctggcgct
421 gtcgccgccg cggctgctgc tgctgctgct gtctctgctg ccagtggcca gggcctcaga
481 ggctgagcac cgtctatttg agcggctgtt tgaagattac aatgagatca tccggcctgt
541 agccaacgtg tctgacccag tcatcatcca tttcgaggtg tccatgtctc agctggtgaa
601 ggtggatgaa gtaaaccaga tcatggagac caacctgtgg ctcaagcaaa tctggaatga
661 ctacaagctg aagtggaacc cctctgacta tggtggggca gagttcatgc gtgtccctgc
721 acagaagatc tggaagccag acattgtgct gtataacaat gctgttgggg atttccaggt
781 ggacgacaag accaaagcct tactcaagta cactggggag gtgacttgga tacctccggc
841 catctttaag agctcctgta aaatcgacgt gacctacttc ccgtttgatt accaaaactg
901 taccatgaag ttcggttcct ggtcctacga taaggcgaaa atcgatctgg tcctgatcgg
961 ctcttccatg aacctcaagg actattggga gagcggcgag tgggccatca tcaaagcccc
1021 aggctacaaa cacgacatca agtacaactg ctgcgaggag atctaccccg acatcacata
1081 ctcgctgtac atccggcgcc tgcccttgtt ctacaccatc aacctcatca tcccctgcct
1141 gctcatctcc ttcctcactg tgctcgtctt ctacctgccc tccgactgcg gtgagaaggt
1201 gaccctgtgc atttctgtcc tcctctccct gacggtgttt ctcctggtga tcactgagac
1261 catcccttcc acctcgctgg tcatccccct gattggagag tacctcctgt tcaccatgat
1321 ttttgtaacc ttgtccatcg tcatcaccgt cttcgtgctc aacgtgcact acagaacccc
1381 gacgacacac acaatgccct catgggtgaa gactgtattc ttgaacctgc tccccagggt
1441 catgttcatg accaggccaa caagcaacga gggcaacgct cagaagccga ggcccctcta
1501 cggtgccgag ctctcaaatc tgaattgctt cagccgcgca gagtccaaag gctgcaagga
1561 gggctacccc tgccaggacg ggatgtgtgg ttactgccac caccgcagga taaaaatctc
1621 caatttcagt gctaacctca cgagaagctc tagttctgaa tctgttgatg ctgtgctgtc
1681 cctctctgct ttgtcaccag aaatcaaaga agccatccaa agtgtcaagt atattgctga
1741 aaatatgaaa gcacaaaatg aagccaaaga gattcaagat gattggaagt atgttgccat
1801 ggtgattgat cgtatttttc tgtgggtttt caccctggtg tgcattctag ggacagcagg
1861 attgtttctg caacccctga tggccaggga agatgcataa gcactaagct gtgtgcctgc
1921 ctgggagact tccttgtgtc agggcaggag gaggctgctt cctagtaaga acgtactttc
1981 tgttatcaag ctaccagctt tgtttttggc atttcgaggt ttacttattt tccacttatc
2041 ttggaatcat gcaaaaaaaa aatgtcaaga gtatttatta ccgataaatg aacatttaac
2101 tagccttttt ggtatggtaa agagatgtca aaatgtgatt ctatgtgatt agtatgctat
2161 gctatggaat atacatgtaa aaatgtttcc ttttagttgt tgaaacaaaa ctggatagaa
2221 aaatgctgtt cagaaatatg aaaagtcatt cagttatcac tacagatctc ccagtaattt
2281 ttcttattta gcccataatc tctttgaagg tttatactaa ttcagcaatc ccccatcgtt
2341 acccatttct taccatgcat ttctcgttct ttactgggtc taaagggcta tgcctccatt
2401 tcagagagct tcaactactt ctcttgcata cttctaaatt atactatgag aaatcatgcc
2461 tagttattca ttgttaatat aactgtctta gtacaccata aactgggtgg attataaaca
2521 acagaaactt ctcagttttg gaggttggga ggtccaaggt caaggcacca gcaaatttgg
2581 tgtctggtga gggtcctctt cctcaaaggg ggggtgcctt ctagctgtgt cctcacatga
2641 ctgaagggac tagctatctc tgtggggtct attttataag ggcactaacc ccattcatga
2701 gagcagagcc cccatggcct aatcaccttt ccaaggcccc accttctatc taagacaatc
2761 acgctgggaa taggtttcaa catatgaatt gggggaggac acatttggac cacagcatga
2821 acctttagaa cagggtttct cagccttagc actacggaca ttttgggctg gataaatatg
2881 tgttggtaca gaatgggggt atcctgtgca ttgtaggatc tttagcagta ccctagcctc
2941 aactcactag atgccaatga cataccttgc ttcttcacca gttatgataa ccaagaatgt
3001 ctccattgtt aaatgtcccc ttaggagcaa aattgcccct ggttgagaaa cattgcttta
3061 gacaaattgt taagagtatc atgtactaca cttctgaaac ttaacgtgat catcaccact
3121 gacagatgat tcacagagag agactgtttg aatcttgtct cactagtttt tcctgtgcaa
3181 aaataaaatg gacagaattg caaaaaaaaa aaaaaaaaaa
//