LOCUS BC096336 3131 bp mRNA linear HUM 04-OCT-2006
DEFINITION Homo sapiens insulin-degrading enzyme, mRNA (cDNA clone MGC:117026
IMAGE:40008464), complete cds.
ACCESSION BC096336
VERSION BC096336.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3131)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3131)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 2 Row: i Column: 19.
FEATURES Location/Qualifiers
source 1..3131
/db_xref="H-InvDB:HIT000335624"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:117026 IMAGE:40008464"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_283"
/note="Vector: pCR-Blunt II-TOPO with reversed insert;
Clone identification sequence tag: CAATTTAT sequenced from
the forward primer"
gene 1..3131
/gene="IDE"
/gene_synonym="INSULYSIN"
/db_xref="GeneID:3416"
/db_xref="HGNC:HGNC:5381"
/db_xref="MIM:146680"
CDS 41..3100
/gene="IDE"
/gene_synonym="INSULYSIN"
/codon_start=1
/product="insulin-degrading enzyme"
/protein_id="AAH96336.1"
/db_xref="GeneID:3416"
/db_xref="HGNC:HGNC:5381"
/db_xref="MIM:146680"
/translation="MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNN
PAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPN
IAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG
ALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF
GTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFS
EVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYL
GHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIL
HMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVL
TAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDE
VIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDK
FFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTI
YGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHA
MYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALG
IMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTD
MQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPP
HYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQ
QYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEF
PCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL"
BASE COUNT 973 a 658 c 668 g 832 t
ORIGIN
1 gaggaagcgt ttgcggtgat cccggcgact gcgctggcta atgcggtacc ggctagcgtg
61 gcttctgcac cccgcactgc ccagcacctt ccgctcagtc ctcggcgccc gcctgccgcc
121 tccggagcgc ctgtgtggtt tccaaaaaaa gacttacagc aaaatgaata atccagccat
181 caagagaata ggaaatcaca ttaccaagtc tcctgaagac aagcgagaat atcgagggct
241 agagctggcc aatggtatca aagtacttct tatcagtgat cccaccacgg ataagtcatc
301 agcagcactt gatgtgcaca taggttcatt gtcggatcct ccaaatattg ctggcttaag
361 tcatttttgt gaacatatgc tttttttggg aacaaagaaa taccctaaag aaaatgaata
421 cagccagttt ctcagtgagc atgcaggaag ttcaaatgcc tttactagtg gagagcatac
481 caattactat tttgatgttt ctcatgaaca cctagaaggt gccctagaca ggtttgcaca
541 gttttttctg tgccccttgt tcgatgaaag ttgcaaagac agagaggtga atgcagttga
601 ttcagaacat gagaagaatg tgatgaatga tgcctggaga ctctttcaat tggaaaaagc
661 tacagggaat cctaaacacc ccttcagtaa atttgggaca ggtaacaaat atactctgga
721 gactagacca aaccaagaag gcattgatgt aagacaagag ctactgaaat tccattctgc
781 ttactattca tccaacttaa tggctgtttg tgttttaggt cgagaatctt tagatgactt
841 gactaatctg gtggtaaagt tattttctga agtagagaac aaaaatgttc cattgccaga
901 atttcctgaa caccctttcc aagaagaaca tcttaaacaa ctttacaaaa tagtacccat
961 taaagatatt aggaatctct atgtgacatt tcccatacct gaccttcaga aatactacaa
1021 atcaaatcct ggtcattatc ttggtcatct cattgggcat gaaggtcctg gaagtctgtt
1081 atcagaactt aagtcaaagg gctgggttaa tactcttgtt ggtgggcaga aggaaggagc
1141 ccgaggtttt atgtttttta tcattaatgt ggacttgacc gaggaaggat tattacatgt
1201 tgaagatata attttgcaca tgtttcaata cattcagaag ttacgtgcag aaggacctca
1261 agaatgggtt ttccaagagt gcaaggactt gaatgctgtt gcttttaggt ttaaagacaa
1321 agagaggcca cggggctata catctaagat tgcaggaata ttgcattatt atcccctaga
1381 agaggtgctc acagcggaat atttactgga agaatttaga cctgacttaa tagagatggt
1441 tctcgataaa ctcagaccag aaaatgtccg ggttgccata gtttctaaat cttttgaagg
1501 aaaaactgat cgcacagaag agtggtatgg aacccagtac aaacaagaag ctataccgga
1561 tgaagtcatc aagaaatggc aaaatgctga cctgaatggg aaatttaaac ttcctacaaa
1621 gaatgaattt attcctacga attttgagat tttaccgtta gaaaaagagg cgacaccata
1681 ccctgctctt attaaggata cagctatgag caaactttgg ttcaaacaag atgataagtt
1741 ttttttgccg aaggcttgtc tcaactttga atttttcagc ccatttgctt atgtggaccc
1801 cttgcactgt aacatggcct atttgtacct tgagctcctc aaagactcac tcaacgagta
1861 tgcatatgca gcagagctag caggcttgag ctatgatctc caaaatacca tctatgggat
1921 gtatctttca gtgaaaggtt acaatgacaa gcagccaatt ttactaaaga agattattga
1981 gaaaatggct acctttgaga ttgatgaaaa aagatttgaa attatcaaag aagcatatat
2041 gcgatctctt aacaatttcc gggctgaaca gcctcaccag catgccatgt actacctccg
2101 cttgctgatg actgaagtgg cctggactaa agatgagtta aaagaagctc tggatgatgt
2161 aacccttcct cgccttaagg ccttcatacc tcagctcctg tcacggctgc acattgaagc
2221 ccttctccat ggaaacataa caaagcaggc tgcattagga attatgcaga tggttgaaga
2281 caccctcatt gaacatgctc ataccaaacc tctccttcca agtcagctgg ttcggtatag
2341 agaagttcag ctccctgaca gaggatggtt tgtttatcag cagagaaatg aagttcacaa
2401 taactgtggc atcgagatat actaccaaac agacatgcaa agcacctcag agaatatgtt
2461 tctggagctc ttctgtcaga ttatctcgga accttgcttc aacaccctgc gcaccaagga
2521 gcagttgggc tatatcgtct tcagcgggcc acgtcgagct aatggcatac agggcttgag
2581 attcatcatc cagtcagaaa agccacctca ctacctagaa agcagagtgg aagctttctt
2641 aattaccatg gaaaagtcca tagaggacat gacagaagag gccttccaaa aacacattca
2701 ggcattagca attcgtcgac tagacaaacc aaagaagcta tctgctgagt gtgctaaata
2761 ctggggagaa atcatctccc agcaatataa ttttgacaga gataacactg aggttgcata
2821 tttaaagaca cttaccaagg aagatatcat caaattctac aaggaaatgt tggcagtaga
2881 tgctccaagg agacataagg tatccgtcca tgttcttgcc agggaaatgg attcttgtcc
2941 tgttgttgga gagttcccat gtcaaaatga cataaatttg tcacaagcac cagccttgcc
3001 acaacctgaa gtgattcaga acatgaccga attcaagcgt ggtctgccac tgtttcccct
3061 tgtgaaacca catattaact tcatggctgc aaaactctga agattcccca tgcatgggaa
3121 agtgcaagtg g
//