LOCUS BC096079 1637 bp mRNA linear HUM 08-DEC-2006
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1,
mRNA (cDNA clone MGC:116717 IMAGE:40000713), complete cds.
ACCESSION BC096079
VERSION BC096079.3
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1637)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1637)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Jun 29, 2006 this sequence version replaced BC096079.2.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 2 Row: l Column: 8.
FEATURES Location/Qualifiers
source 1..1637
/db_xref="H-InvDB:HIT000335367"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:116717 IMAGE:40000713"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_282"
/note="Vector: pCR-Blunt II-TOPO; Clone identification
sequence tag: GGTTTTTC sequenced from the reverse primer"
gene 1..1637
/gene="PFKFB1"
/gene_synonym="F6PK"
/gene_synonym="HL2K"
/gene_synonym="MGC116717"
/db_xref="GeneID:5207"
/db_xref="HGNC:HGNC:8872"
/db_xref="MIM:311790"
CDS 73..1488
/gene="PFKFB1"
/gene_synonym="F6PK"
/gene_synonym="HL2K"
/gene_synonym="MGC116717"
/codon_start=1
/product="6-phosphofructo-2-kinase/fructose-2,
6-biphosphatase 1"
/protein_id="AAH96079.1"
/db_xref="GeneID:5207"
/db_xref="HGNC:HGNC:8872"
/db_xref="MIM:311790"
/translation="MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTM
VIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEAL
QIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESI
CNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYI
KIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGL
SVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVC
EEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVIC
HQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREK
PENVDITREPEEALDTVPAHY"
BASE COUNT 441 a 431 c 405 g 360 t
ORIGIN
1 ggacaggtag taagatagga agtgaggcca ggtaccttgt gggcagtgat gtcattcggt
61 gcgactccta agatgtctcc agagatggga gagctcaccc aaaccaggtt gcagaagatc
121 tggattccac acagcagcgg cagcagcagg ctgcaacgga gaaggggctc atccataccc
181 cagtttacca attcccccac aatggtgatc atggtgggtt taccagctcg aggcaagacc
241 tatatctcca caaagctcac acgatatctc aactggatag gaacaccaac taaagtgttt
301 aatttaggcc agtatcgacg agaggcagtg agctacaaga actatgaatt ctttcttcca
361 gacaacatgg aagccctgca aatcaggaag cagtgcgccc tggcagccct gaaggatgtt
421 cacaactatc tcagccatga ggaaggtcat gttgcggttt ttgatgccac caacactacc
481 agagaacgac ggtcactgat cctgcagttt gcaaaagaac atggttacaa ggtgtttttc
541 attgagtcca tttgtaatga ccctggcata attgcagaaa acatcaggca agtgaaactt
601 ggcagccctg attatataga ctgtgaccgg gaaaaggttc tggaagactt tctaaagaga
661 attgagtgct atgaggtcaa ctaccaaccc ttggatgagg aactggacag ccacctgtcc
721 tacatcaaga tcttcgacgt gggcacacgc tacatggtga accgagtgca ggatcacatc
781 cagagccgca cagtctacta cctcatgaat atccatgtca cacctcgctc catctacctt
841 tgccgacatg gcgagagtga actcaacatc agaggccgca tcggaggtga ctctggcctc
901 tcagttcgcg gcaagcagta tgcctatgcc ctggccaact tcattcagtc ccagggcatc
961 agctccctga aggtgtggac cagtcacatg aagaggacca tccagacagc tgaggccctg
1021 ggtgtcccct atgagcagtg gaaggccctg aatgagattg atgcgggtgt ctgtgaggag
1081 atgacctatg aagaaatcca ggaacattac cctgaagaat ttgcactgcg agaccaagat
1141 aaatatcgct accgctatcc caagggagag tcctatgagg atctggttca gcgtctggag
1201 ccagtgataa tggagctaga acgacaggag aatgtactgg tgatctgcca ccaggctgtc
1261 atgcggtgcc tcctggccta tttcctggat aaaagttcag atgagcttcc atatctcaag
1321 tgccctctgc acacagtgct caaactcact cctgtggctt atggctgcaa agtggaatcc
1381 atctacctga atgtggaggc cgtgaacaca caccgggaga agcctgagaa tgtggacatc
1441 acccgggaac ctgaggaagc cctggatact gtcccagccc actactgagc cctttccaag
1501 aagtcaaact gcctgtgtcc tcatcgcctt ccacctttag gaaatgctat ctttgctctt
1561 ctcctactct gccttggcct cactgaggca ccccacttcc agtgaagaag tcctccgcaa
1621 ctcccaaaca agcctcg
//