LOCUS BC096077 1048 bp mRNA linear HUM 04-OCT-2006
DEFINITION Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1,
mRNA (cDNA clone MGC:116715 IMAGE:40000710), complete cds.
ACCESSION BC096077
VERSION BC096077.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1048)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1048)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 2 Row: k Column: 8.
FEATURES Location/Qualifiers
source 1..1048
/db_xref="H-InvDB:HIT000335365"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:116715 IMAGE:40000710"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_283"
/note="Vector: pCR-Blunt II-TOPO with reversed insert;
Clone identification sequence tag: CAACTTAT sequenced from
the forward primer"
gene 1..1048
/gene="PFKFB1"
/db_xref="GeneID:5207"
/db_xref="HGNC:HGNC:8872"
/db_xref="MIM:311790"
CDS 84..899
/gene="PFKFB1"
/codon_start=1
/product="PFKFB1 protein"
/protein_id="AAH96077.1"
/db_xref="GeneID:5207"
/db_xref="HGNC:HGNC:8872"
/db_xref="MIM:311790"
/translation="MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTM
VIMVGLPARGKTYISTKLTRYLNWIGTPTKDNMEALQIRKQCALAALKDVHNYLSHEE
GHVAVFDATNTTRERRSLILQFAKEHGYKGVCEEMTYEEIQEHYPEEFALRDQDKYRY
RYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCP
LHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY"
BASE COUNT 283 a 286 c 256 g 223 t
ORIGIN
1 gcccttcacc cggacaggta gtaagatagg aagtgaggcc aggtaccttg tgggcagtga
61 tgtcattcgg tgcgactcct aagatgtctc cagagatggg agagctcacc caaaccaggt
121 tgcagaagat ctggattcca cacagcagcg gcagcagcag gctgcaacgg agaaggggct
181 catccatacc ccagtttacc aattccccca caatggtgat catggtgggt ttaccagctc
241 gaggcaagac ctatatctcc acaaagctca cacgatatct caactggata ggaacaccaa
301 ctaaagacaa catggaagcc ctgcaaatca ggaagcagtg cgccctggca gccctgaagg
361 atgttcacaa ctatctcagc catgaggaag gtcatgttgc ggtttttgat gccaccaaca
421 ctaccagaga acgacggtca ctgatcctgc agtttgcaaa agaacatggt tacaagggtg
481 tctgtgagga gatgacctat gaagaaatcc aggaacatta ccctgaagaa tttgcactgc
541 gagaccaaga taaatatcgc taccgctatc ccaagggaga gtcctatgag gatctggttc
601 agcgtctgga gccagtgata atggagctag aacgacagga gaatgtactg gtgatctgcc
661 accaggctgt catgcggtgc ctcctggcct atttcctgga taaaagttca gatgagcttc
721 catatctcaa gtgccctctg cacacagtgc tcaaactcac tcctgtggct tatggctgca
781 aagtggaatc catctacctg aatgtggagg ccgtgaacac acaccgggag aagcctgaga
841 atgtggacat cacccgggaa cctgaggaag ccctggatac tgtcccagcc cactactgag
901 ccctttccaa gaagtcaaac tgcctgtgtc ctcatcgcct tccaccttta ggaaatgcta
961 tctttgctct tctcctactc tgccttggcc tcactgaggc accccacttc cagtgaagaa
1021 gtcctccgca actcccaaac aagcctcg
//