LOCUS BC095453 2109 bp mRNA linear HUM 01-JUN-2005
DEFINITION Homo sapiens tryptophanyl-tRNA synthetase, mRNA (cDNA clone
MGC:111225 IMAGE:30418785), complete cds.
ACCESSION BC095453
VERSION BC095453.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2109)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2109)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-MAY-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 68 Row: d Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47419913.
FEATURES Location/Qualifiers
source 1..2109
/db_xref="H-InvDB:HIT000335246"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:111225 IMAGE:30418785"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2109
/gene="WARS"
/gene_synonym="GAMMA-2"
/gene_synonym="IFP53"
/db_xref="GeneID:7453"
/db_xref="MIM:191050"
CDS 269..1684
/gene="WARS"
/gene_synonym="GAMMA-2"
/gene_synonym="IFP53"
/codon_start=1
/product="WARS protein"
/protein_id="AAH95453.1"
/db_xref="GeneID:7453"
/db_xref="MIM:191050"
/translation="MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLV
SLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDK
LIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYT
GRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVEN
AKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIG
KISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCVIDQDPYFRMTRDVAPRIGYPKPAL
LHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGG
NCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLLPLIAEHQARRK
EVTDEIVKEFMTPRKLSFDFQ"
BASE COUNT 580 a 535 c 487 g 507 t
ORIGIN
1 actctcagca gctcaactgc ccagcgtgac cagtggccac ctctgcagtg tcttccacaa
61 cctggagtgt actatttgtt aaacaggtga atctacttct cttaacaaag tctcaatgtc
121 ctatttgcaa tttatgtggt aaacactgaa gacaatggtc cttaaccttt tggcatctca
181 gcctcctttc gaaagtcttg actcgtctgc tgaacaaatc ctctgacctc aggccggctg
241 tgaacgtagt tcctgagaga tagcaaacat gcccaacagt gagcccgcat ctctgctgga
301 gctgttcaac agcatcgcca cacaagggga gctcgtaagg tccctcaaag cgggaaatgc
361 gtcaaaggat gaaattgatt ctgcagtaaa gatgttggtg tcattaaaaa tgagctacaa
421 agctgccgcg ggggaggatt acaaggctga ctgtcctcca gggaacccag cacctaccag
481 taatcatggc ccagatgcca cagaagctga agaggatttt gtggacccat ggacagtaca
541 gacaagcagt gcaaaaggca tagactacga taagctcatt gttcggtttg gaagtagtaa
601 aattgacaaa gagctaataa accgaataga gagagccacc ggccaaagac cacaccactt
661 cctgcgcaga ggcatcttct tctcacacag agatatgaat caggttcttg atgcctatga
721 aaataagaag ccattttatc tgtacacggg ccggggcccc tcttctgaag caatgcatgt
781 aggtcacctc attccattta ttttcacaaa gtggctccag gatgtattta acgtgccctt
841 ggtcatccag atgacggatg acgagaagta tctgtggaag gacctgaccc tggaccaggc
901 ctatagctat gctgtggaga atgccaagga catcatcgcc tgtggctttg acatcaacaa
961 gactttcata ttctctgacc tggactacat ggggatgagc tcaggtttct acaaaaatgt
1021 ggtgaagatt caaaagcatg ttaccttcaa ccaagtgaaa ggcattttcg gcttcactga
1081 cagcgactgc attgggaaga tcagttttcc tgccatccag gctgctccct ccttcagcaa
1141 ctcattccca cagatcttcc gagacaggac ggatatccag tgccttatcc catgtgtcat
1201 tgaccaggat ccttacttta gaatgacaag ggacgtcgcc cccaggatcg gctatcctaa
1261 accagccctg ctgcactcca ccttcttccc agccctgcag ggcgcccaga ccaaaatgag
1321 tgccagcgac cccaactcct ccatcttcct caccgacacg gccaagcaga tcaaaaccaa
1381 ggtcaataag catgcgtttt ctggagggag agacaccatc gaggagcaca ggcagtttgg
1441 gggcaactgt gatgtggacg tgtctttcat gtacctgacc ttcttcctcg aggacgacga
1501 caagctcgag cagatcagga aggattacac cagcggagcc atgctcaccg gtgagctcaa
1561 gaaggcactc atagaggttc tgctgccctt gatcgcagag caccaggccc ggcgcaagga
1621 ggtcacggat gagatagtga aagagttcat gactccccgg aagctgtcct tcgactttca
1681 gtagcactcg ttttacatat gcttataaaa gaagtgatgt atcagtaatg tatcaataat
1741 cccagcccag tcaaagcacc gccacctgta ggcttctgtc tcatggtaat tactgggcct
1801 ggcctctgta agcctgtgta tgttatcaat actgtttctt cctgtgagtt ccattatttc
1861 tatctcttat gggcaaagca ttgtgggtaa ttggtgctgg ctaacattgc atggtcggat
1921 agagaagtcc agctgtgagt ctctccccaa agcagcccca cagtggagcc tttggctgga
1981 agtccatggg ccaccctgtt cttgtccatg gaggactccg agggttccaa gtatactctt
2041 aagacccact ctgtttaaaa atatatattc tatgtatgcg aaaaaaaaaa aaaaaaaaaa
2101 aaaaaaaaa
//