LOCUS BC094767 1879 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:104654
IMAGE:5296639), complete cds.
ACCESSION BC094767
VERSION BC094767.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1879)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1879)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-MAY-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 198 Row: e Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33286417.
FEATURES Location/Qualifiers
source 1..1879
/db_xref="H-InvDB:HIT000335068"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:104654 IMAGE:5296639"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..1879
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="MIM:179050"
CDS 145..1245
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="PKM2 protein"
/protein_id="AAH94767.1"
/db_xref="GeneID:5315"
/db_xref="MIM:179050"
/translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPSKKGV
NLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKI
ISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVI
CATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAR
EAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQV
ARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKA
RGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP"
BASE COUNT 418 a 544 c 514 g 403 t
ORIGIN
1 gcccacttcg cagtccctag ttctccctac tgccgctcca gtgccagagc ccctccgaag
61 gcggccagga cctccaacca cgcacaagtc tgcagctctc cccaactttc cgttcagctc
121 agtctccgag ggacctcagc agccatgtcg aagccccata gtgaagccgg gactgccttc
181 attcagaccc agcagctgca cgcagccatg gctgacacat tcctggagca catgtgccgc
241 ctggacattg attcaccacc cagcaagaag ggtgtgaacc ttcctggggc tgctgtggac
301 ttgcctgctg tgtcggagaa ggacatccag gatctgaagt ttggggtcga gcaggatgtt
361 gatatggtgt ttgcgtcatt catccgcaag gcatctgatg tccatgaagt taggaaggtc
421 ctgggagaga agggaaagaa catcaagatt atcagcaaaa tcgagaatca tgagggggtt
481 cggaggtttg atgaaatcct ggaggccagt gatgggatca tggtggctcg tggtgatcta
541 ggcattgaga ttcctgcaga gaaggtcttc cttgctcaga agatgatgat tggacggtgc
601 aaccgagctg ggaagcctgt catctgtgct actcagatgc tggagagcat gatcaagaag
661 ccccgcccca ctcgggctga aggcagtgat gtggccaatg cagtcctgga tggagccgac
721 tgcatcatgc tgtctggaga aacagccaaa ggggactatc ctctggaggc tgtgcgcatg
781 cagcacctga tagctcgtga ggctgaggca gccatgttcc accgcaagct gtttgaagaa
841 cttgtgcgag cctcaagtca ctccacagac ctcatggaag ccatggccat gggcagcgtg
901 gaggcttctt ataagtgttt agcagcagct ttgatagttc tgacggagtc tggcaggtct
961 gctcaccagg tggccagata ccgcccacgt gcccccatca ttgctgtgac ccggaatccc
1021 cagacagctc gtcaggccca cctgtaccgt ggcatcttcc ctgtgctgtg caaggaccca
1081 gtccaggagg cctgggctga ggacgtggac ctccgggtga actttgccat gaatgttggc
1141 aaggcccgag gcttcttcaa gaagggagat gtggtcattg tgctgaccgg atggcgccct
1201 ggctccggct tcaccaacac catgcgtgtt gttcctgtgc cgtgatggac cccagagccc
1261 ctcctccagc ccctgtccca cccccttccc ccagcccatc cattaggcca gcaacgcttg
1321 tagaactcac tctgggctgt aacgtggcac tggtaggttg ggacaccagg gaagaagatc
1381 aacgcctcac tgaaacatgg ctgtgtttgc agcctgctct agtgggacag cccagagcct
1441 ggctgcccat catgtggccc cacccaatca agggaagaag gaggaatgct ggactggagg
1501 cccctggagc cagatggcaa gagggtgaca gcttcctttc ctgtgtgtac tctgtccagt
1561 tcctttagaa aaaatggatg cccagaggac tcccaaccct ggcttggggt caagaaacag
1621 ctagcaagag ttaggggcct tagggcactg ggctgttgtt ccattgaagc cgactctggc
1681 cctggccctt acttgcttct ctagctctct aggcctctcc agtttgcacc tgtccccacc
1741 ctccactcag ctgtcctgca gcaaacactc caccctccac cttccatttt cccccactac
1801 tgcagcacct ccaggcctgt tgctatagag cctacctgta tgtcaataaa caacagctga
1861 agcaaaaaaa aaaaaaaaa
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