LOCUS BC093731 3759 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens epidermal growth factor (beta-urogastrone), mRNA (cDNA
clone MGC:120766 IMAGE:7939576), complete cds.
ACCESSION BC093731
VERSION BC093731.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3759)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3759)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-APR-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Mike Brownstein, NIMH
cDNA Library Preparation: British Columbia Cancer Research Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRCB Plate: 1 Row: e Column: 14.
FEATURES Location/Qualifiers
source 1..3759
/db_xref="H-InvDB:HIT000334706"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:120766 IMAGE:7939576"
/tissue_type="Colon, PCR rescued clones"
/clone_lib="NIH_MGC_295"
/note="Vector: pCR4-TOPO with reversed insert; Clone
identification sequence tag: TCGATGCA"
gene 1..3759
/gene="EGF"
/gene_synonym="URG"
/db_xref="GeneID:1950"
/db_xref="HGNC:HGNC:3229"
/db_xref="MIM:131530"
CDS 96..3719
/gene="EGF"
/gene_synonym="URG"
/codon_start=1
/product="epidermal growth factor (beta-urogastrone)"
/protein_id="AAH93731.1"
/db_xref="GeneID:1950"
/db_xref="HGNC:HGNC:3229"
/db_xref="MIM:131530"
/translation="MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGP
APFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVF
LNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYP
ANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQ
YNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHT
GKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDL
QSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDG
KRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQL
CVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLS
QQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIG
RRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIE
SSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQN
DVGHPFAVAVFEDYVWFSDWAMPSVIRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKP
GADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKN
QVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQ
CLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDID
ECQLGVHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNS
DSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQ
QKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTED
GMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGC
WIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQME
LTQ"
BASE COUNT 1035 a 768 c 972 g 984 t
ORIGIN
1 cagccgcatc tggggtcaat catactcacc ttgcccgggc catgctccag caaaatcaag
61 ctgttttctt ttgaaagttc aaactcatca agattatgct gctcactctt atcattctgt
121 tgccagtagt ttcaaaattt agttttgtta gtctctcagc accgcagcac tggagctgtc
181 ctgaaggtac tctcgcagga aatgggaatt ctacttgtgt gggtcctgca cccttcttaa
241 ttttctccca tggaaatagt atctttagga ttgacacaga aggaaccaat tatgagcaat
301 tggtggtgga tgctggtgtc tcagtgatca tggattttca ttataatgag aaaagaatct
361 attgggtgga tttagaaaga caacttttgc aaagagtttt tctgaatggg tcaaggcaag
421 agagagtatg taatatagag aaaaatgttt ctggaatggc aataaattgg ataaatgaag
481 aagttatttg gtcaaatcaa caggaaggaa tcattacagt aacagatatg aaaggaaata
541 attcccacat tcttttaagt gctttaaaat atcctgcaaa tgtagcagtt gatccagtag
601 aaaggtttat attttggtct tcagaggtgg ctggaagcct ttatagagca gatctcgatg
661 gtgtgggagt gaaggctctg ttggagacat cagagaaaat aacagctgtg tcattggatg
721 tgcttgataa gcggctgttt tggattcagt acaacagaga aggaagcaat tctcttattt
781 gctcctgtga ttatgatgga ggttctgtcc acattagtaa acatccaaca cagcataatt
841 tgtttgcaat gtcccttttt ggtgaccgta tcttctattc aacatggaaa atgaagacaa
901 tttggatagc caacaaacac actggaaagg acatggttag aattaacctc cattcatcat
961 ttgtaccact tggtgaactg aaagtagtgc atccacttgc acaacccaag gcagaagatg
1021 acacttggga gcctgagcag aaactttgca aattgaggaa aggaaactgc agcagcactg
1081 tgtgtgggca agacctccag tcacacttgt gcatgtgtgc agagggatac gccctaagtc
1141 gagaccggaa gtactgtgaa gatgttaatg aatgtgcttt ttggaatcat ggctgtactc
1201 ttgggtgtaa aaacacccct ggatcctatt actgcacgtg ccctgtagga tttgttctgc
1261 ttcctgatgg gaaacgatgt catcaacttg tttcctgtcc acgcaatgtg tctgaatgca
1321 gccatgactg tgttctgaca tcagaaggtc ccttatgttt ctgtcctgaa ggctcagtgc
1381 ttgagagaga tgggaaaaca tgtagcggtt gttcctcacc cgataatggt ggatgtagcc
1441 agctctgcgt tcctcttagc ccagtatcct gggaatgtga ttgctttcct gggtatgacc
1501 tacaactgga tgaaaaaagc tgtgcagctt caggaccaca accatttttg ctgtttgcca
1561 attctcaaga tattcgacac atgcattttg atggaacaga ctatggaact ctgctcagcc
1621 agcagatggg aatggtttat gccctagatc atgaccctgt ggaaaataag atatactttg
1681 cccatacagc cctgaagtgg atagagagag ctaatatgga tggttcccag cgagaaaggc
1741 ttattgagga aggagtagat gtgccagaag gtcttgctgt ggactggatt ggccgtagat
1801 tctattggac agacagaggg aaatctctga ttggaaggag tgatttaaat gggaaacgtt
1861 ccaaaataat cactaaggag aacatctctc aaccacgagg aattgctgtt catccaatgg
1921 ccaagagatt attctggact gatacaggga ttaatccacg aattgaaagt tcttccctcc
1981 aaggccttgg ccgtctggtt atagccagct ctgatctaat ctggcccagt ggaataacga
2041 ttgacttctt aactgacaag ttgtactggt gcgatgccaa gcagtctgtg attgaaatgg
2101 ccaatctgga tggttcaaaa cgccgaagac ttacccagaa tgatgtaggt cacccatttg
2161 ctgtagcagt gtttgaggat tatgtgtggt tctcagattg ggctatgcca tcagtaataa
2221 gagtaaacaa gaggactggc aaagatagag tacgtctcca aggcagcatg ctgaagccct
2281 catcactggt tgtggttcat ccattggcaa aaccaggagc agatccctgc ttatatcaaa
2341 acggaggctg tgaacatatt tgcaaaaaga ggcttggaac tgcttggtgt tcgtgtcgtg
2401 aaggttttat gaaagcctca gatgggaaaa cgtgtctggc tctggatggt catcagctgt
2461 tggcaggtgg tgaagttgat ctaaagaacc aagtaacacc attggacatc ttgtccaaga
2521 ctagagtgtc agaagataac attacagaat ctcaacacat gctagtggct gaaatcatgg
2581 tgtcagatca agatgactgt gctcctgtgg gatgcagcat gtatgctcgg tgtatttcag
2641 agggagagga tgccacatgt cagtgtttga aaggatttgc tggggatgga aaactatgtt
2701 ctgatataga tgaatgtgag atgggtgtcc cagtgtgccc ccctgcctcc tccaagtgca
2761 tcaacaccga aggtggttat gtctgccggt gctcagaagg ctaccaagga gatgggattc
2821 actgtcttga tattgatgag tgccaactgg gggtgcacag ctgtggagag aatgccagct
2881 gcacaaatac agagggaggc tatacctgca tgtgtgctgg acgcctgtct gaaccaggac
2941 tgatttgccc tgactctact ccaccccctc acctcaggga agatgaccac cactattccg
3001 taagaaatag tgactctgaa tgtcccctgt cccacgatgg gtactgcctc catgatggtg
3061 tgtgcatgta tattgaagca ttggacaagt atgcatgcaa ctgtgttgtt ggctacatcg
3121 gggagcgatg tcagtaccga gacctgaagt ggtgggaact gcgccacgct ggccacgggc
3181 agcagcagaa ggtcatcgtg gtggctgtct gcgtggtggt gcttgtcatg ctgctcctcc
3241 tgagcctgtg gggggcccac tactacagga ctcagaagct gctatcgaaa aacccaaaga
3301 atccttatga ggagtcgagc agagatgtga ggagtcgcag gcctgctgac actgaggatg
3361 ggatgtcctc ttgccctcaa ccttggtttg tggttataaa agaacaccaa gacctcaaga
3421 atgggggtca accagtggct ggtgaggatg gccaggcagc agatgggtca atgcaaccaa
3481 cttcatggag gcaggagccc cagttatgtg gaatgggcac agagcaaggc tgctggattc
3541 cagtatccag tgataagggc tcctgtcccc aggtaatgga gcgaagcttt catatgccct
3601 cctatgggac acagaccctt gaagggggtg tcgagaagcc ccattctctc ctatcagcta
3661 acccattatg gcaacaaagg gccctggacc caccacacca aatggagctg actcagtgaa
3721 aactggaatt aaaaggaaag tcaagaagaa tgaactatg
//