LOCUS BC093069 999 bp mRNA linear HUM 19-APR-2005
DEFINITION Homo sapiens proteasome (prosome, macropain) subunit, alpha type,
4, mRNA (cDNA clone MGC:111191 IMAGE:30414795), complete cds.
ACCESSION BC093069
VERSION BC093069.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 999)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 999)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-APR-2005) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Narayan Bhat
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 68 Row: c Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23110940.
FEATURES Location/Qualifiers
source 1..999
/db_xref="H-InvDB:HIT000334560"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:111191 IMAGE:30414795"
/tissue_type="Blood, peripheral blood mononuclear cells
(PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
6 hr after stimulation with PMA and Ionomycin. These
reagents are used to activate T- cells that results in
lymphokine and cytokine production."
/clone_lib="NIH_MGC_191"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..999
/gene="PSMA4"
/gene_synonym="HC9"
/gene_synonym="HsT17706"
/gene_synonym="MGC12467"
/gene_synonym="MGC24813"
/db_xref="GeneID:5685"
CDS 141..713
/gene="PSMA4"
/gene_synonym="HC9"
/gene_synonym="HsT17706"
/gene_synonym="MGC12467"
/gene_synonym="MGC24813"
/codon_start=1
/product="PSMA4 protein"
/protein_id="AAH93069.1"
/db_xref="GeneID:5685"
/translation="MACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALC
DIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVS
MLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQK
EVEQLIKKHEEEEAKAEREKKEKEQKEKDK"
BASE COUNT 347 a 169 c 220 g 263 t
ORIGIN
1 ggtggtttat tcttccgtgg agttaagggc tccgtggaca tctcaggtct tcagggtctt
61 ccatctggaa ctatataaag ttcagaaaac atgtctcgaa gatatgactc caggaccact
121 atattttctc cagaagggac atggcttgca gtgtggcagg cataacttct gatgctaatg
181 ttctgactaa tgaactaagg ctcattgctc aaaggtattt attacagtat caggagccaa
241 taccttgtga gcagttggtt acagcgctgt gtgatatcaa acaagcttat acacaatttg
301 gaggaaaacg tccctttggt gtttcattgc tgtacattgg ctgggataag cactatggct
361 ttcagctcta tcagagtgac cctagtggaa attacggggg atggaaggcc acatgcattg
421 gaaataatag cgctgcagct gtgtcaatgt tgaaacaaga ctataaagaa ggagaaatga
481 ccttgaagtc agcacttgct ttagctatca aagtactaaa taagaccatg gatgttagta
541 aactctctgc tgaaaaagtg gaaattgcaa cactaacaag agagaatgga aagacagtaa
601 tcagagttct caaacaaaaa gaagtggagc agttgatcaa aaaacacgag gaagaagaag
661 ccaaagctga gcgtgagaag aaagaaaaag aacagaaaga aaaggataaa tagaatcaga
721 gattttatta ctcatttggg gcaccatttc agtgtaaaag cagtcctact cttccacact
781 aggaaggctt tacttttttt aactggtgca gtgggaaaat aggacattac atactgaatt
841 gggtccttgt catttctgtc caattgaata ctttattgta acgatgatgg ttacccttca
901 tggacgtctt aatcttccac acacatcccc tttttttgga ataaaatttg gaaaatggaa
961 atgaaggagt aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//