LOCUS BC086957 4311 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens fragile X mental retardation 1, mRNA (cDNA clone
MGC:87458 IMAGE:30347992), complete cds.
ACCESSION BC086957
VERSION BC086957.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4311)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4311)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Stefan Hansson
cDNA Library Preparation: Michael Brownstein / Ted Usdin
Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 168 Row: p Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 50053960.
FEATURES Location/Qualifiers
source 1..4311
/db_xref="H-InvDB:HIT000266151"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87458 IMAGE:30347992"
/tissue_type="Placenta, normal"
/clone_lib="NIH_MGC_147"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4311
/gene="FMR1"
/gene_synonym="FMRP"
/gene_synonym="FRAXA"
/db_xref="GeneID:2332"
/db_xref="MIM:309550"
CDS 260..2020
/gene="FMR1"
/gene_synonym="FMRP"
/gene_synonym="FRAXA"
/codon_start=1
/product="FMR1 protein"
/protein_id="AAH86957.1"
/db_xref="GeneID:2332"
/db_xref="MIM:309550"
/translation="MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPF
HDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDAT
YNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVT
YDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASR
FHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKAR
SFLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEAENEKNVPQEEEIMP
PNSLPSNNSRVGPNAPEEKKHLDIKENSTHFSQPNSTKVQRGMVPFVFVGTKDSIANA
TVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKSYVTDDGQGMGR
GSRPYRNRGHGRRGPGYTSAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGND
DHSRTDNRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRN
QKKEKPDSVDGQQPLVNGVP"
BASE COUNT 1335 a 706 c 983 g 1287 t
ORIGIN
1 acttccggtg gagggccgcc tctgagcggg cggcgggccg acggcgagcg cgggcggcgg
61 cggtgacgga ggcgccgctg ccagggggcg tgcggcagcg cggcggcggc ggcggcggcg
121 gcggcggagg cggcggcggc ggcggcggcg gcggcggcgg aggcggcggc ggcggcggcg
181 gcggcggcgg ctgggcctcg agcgcccgca gcccacctct cgggggcggg ctcccggcgc
241 tagcagggct gaagagaaga tggaggagct ggtggtggaa gtgcggggct ccaatggcgc
301 tttctacaag gcatttgtaa aggatgttca tgaagattca ataacagttg catttgaaaa
361 caactggcag cctgataggc agattccatt tcatgatgtc agattcccac ctcctgtagg
421 ttataataaa gatataaatg aaagtgatga agttgaggtg tattccagag caaatgaaaa
481 agagccttgc tgttggtggt tagctaaagt gaggatgata aagggtgagt tttatgtgat
541 agaatatgca gcatgtgatg caacttacaa tgaaattgtc acaattgaac gtctaagatc
601 tgttaatccc aacaaacctg ccacaaaaga tactttccat aagatcaagc tggatgtgcc
661 agaagactta cggcaaatgt gtgccaaaga ggcggcacat aaggatttta aaaaggcagt
721 tggtgccttt tctgtaactt atgatccaga aaattatcag cttgtcattt tgtccatcaa
781 tgaagtcacc tcaaagcgag cacatatgct gattgacatg cactttcgga gtctgcgcac
841 taagttgtct ctgataatga gaaatgaaga agctagtaag cagctggaga gttcaaggca
901 gcttgcctcg agatttcatg aacagtttat cgtaagagaa gatctgatgg gtctagctat
961 tggtactcat ggtgctaata ttcagcaagc tagaaaagta cctggggtca ctgctattga
1021 tctagatgaa gatacctgca catttcatat ttatggagag gatcaggatg cagtgaaaaa
1081 agctagaagc tttctcgaat ttgctgaaga tgtaatacaa gttccaagga acttagtagg
1141 caaagtaata ggaaaaaatg gaaagctgat tcaggagatt gtggacaagt caggagttgt
1201 gagggtgagg attgaggctg aaaatgagaa aaatgttcca caagaagagg aaattatgcc
1261 accaaattcc cttccttcca ataattcaag ggttggacct aatgccccag aagaaaaaaa
1321 acatttagat ataaaggaaa acagcaccca tttttctcaa cctaacagta caaaagtcca
1381 gaggggtatg gtaccatttg tttttgtggg aacaaaggac agcatcgcta atgccactgt
1441 tcttttggat tatcacctga actatttaaa ggaagtagac cagttgcgtt tggagagatt
1501 acaaattgat gagcagttgc gacagattgg agctagttct agaccaccac caaatcgtac
1561 agataaggaa aaaagctatg tgactgatga tggtcaagga atgggtcgag gtagtagacc
1621 ttacagaaat agggggcacg gcagacgcgg tcctggatat acttcagctc caacagagga
1681 agagagggag agcttcctgc gcagaggaga cggacggcgg cgtggagggg gaggaagagg
1741 acaaggagga agaggacgtg gaggaggctt caaaggaaac gacgatcact cccgaacaga
1801 taatcgtcca cgtaatccaa gagaggctaa aggaagaaca acagatggat cccttcagat
1861 cagagttgac tgcaataatg aaaggagtgt ccacactaaa acattacaga atacctccag
1921 tgaaggtagt cggctgcgca cgggtaaaga tcgtaaccag aagaaagaga agccagacag
1981 cgtggatggt cagcaaccac tcgtgaatgg agtaccctaa actgcataat tctgaagtta
2041 tatttcctat accatttccg taattcttat tccatattag aaaactttgt taggccaaag
2101 acaaatagta ggcaagatgg cacagggcat gaaatgaaca caaattatgc taagaatttt
2161 ttattttttg gtattggcca taagcaacaa ttttcagatt tgcacaaaaa gataccttaa
2221 aatttgaaac attgctgtta aaactactta gcacttcagg gcagatttta gttttatttt
2281 ctaaagtact gagcagtgat attctttgtt aatttggacc attttcctgc attgggtgat
2341 cattcaccag tacattctca gtttttctta atatatagca tttatggtaa tcatattaga
2401 cttctgtttt caatctcgta tagaagtctt catgaaatgc tatgtcattt catgtcctgt
2461 gtcagtttat gttttggtcc acttttccag tattttagtg gaccctgaaa tgtgtgtgat
2521 gtgacatttg tcattttcat tagcaaaaaa agttgtatga tctgtgcctt ttttatatct
2581 tggcaggtag gaatattata tttggatgca gagttcaggg aagataagtt ggaaacacta
2641 aatgttaaag atgtagcaaa ccctgtcaaa cattagtact ttatagaaga atgcatgctt
2701 tccatatttt tttccttaca taaacatcag gttaggcagt ataaagaata ggacttgttt
2761 ttgtttttgt tttgttgcac tgaagtttga taaatagtgt tattgagaga gatgtgtaat
2821 ttttctgtat agacaggaga agaaagaact atcttcatct gagagaggct aaaatgtttt
2881 cagctaggaa caaatcttcc tggtcgaaag ttagtaggat atgcctgctc tttggcctga
2941 tgaccaattt taacttagag cttttttttt tttaattttg tctgccccaa gttttgtgaa
3001 atttttcata ttttaatttc aagcttattt tggagagata ggaaggtcat ttccatgtat
3061 gcataataat cctgcaaagt acaggtactt tgtctaagaa acattggaag caggttaaat
3121 gttttgtaaa ctttgaaata tatggtctaa tgtttaagca gaattggaaa agactaagat
3181 cggttaacaa ataataactt ttttttcttt ttttcttttg ttttttgaag tgttggggtt
3241 tggttttgtt ttttgagtct ttttttttta agtggaattt attgaggaaa aatatgtgaa
3301 ggaccttcac tctaagatgt tatatttttc ttaaaaagta actcctagta ggggtaccac
3361 tgaatctgta cagagccgta aaaactgaag ttctgcctct gatgtatttt gtgagtttgt
3421 ttctttgaat tttcatttta cagttacttt tccttgcata caaacaagca tataaaatgg
3481 caacaaactg cacatgattt cacaaatatt aaaaagtctt ttaaaaagta ttgccaaaca
3541 ttaatgttga tttctagtta tttattctgg gaatgtatag tatttgaaaa cagaaattgg
3601 taccttgcac acatcatctg taagctgttt ggttttaaaa tactgtagat aattaaccaa
3661 ggtagaatga ccttgtaatg taactgctct tgggcaatat tctctgtaca tattagcgac
3721 aacagattgg attttatgtt gacatttgtt tggttatagt gcaatatatt ttgtatgcaa
3781 gcagtttcaa taaagtttga tcttcctctg ctaaattgat gttgatgcaa tccttacaaa
3841 tgattgcttt taaaatttta agctaggaaa agaaatctat agaaagtgtt ctgttacaaa
3901 atgtaactgt taccattgga aatttcacgt cataggaagt tagcctttat ctaccaactt
3961 tcaagaactt gtttaataaa gcgaaaaact caaccaaatg gtacaaaacc acagtgtacc
4021 attaaaatat gcactaagtc tcttttttac aaaggctgta ttcagcaagg cgctaacttg
4081 cttaaatgtg aattactaac ttctaaaact gtactttgat tcacatgttt tcaaatggag
4141 ttggagttca ttcatattac aatatttgtg tgctaaacgt gtatgttttt cagttcaaag
4201 tcatgatgtt tttaaaatct tattaaagtt tcaaaaatct gaagattgtt tatctagatg
4261 taaattttta ttaaaaagtt acacttatga aaaaccaaaa aaaaaaaaaa a
//