LOCUS BC084579 5266 bp mRNA linear HUM 27-OCT-2006
DEFINITION Homo sapiens chromosome 18 open reading frame 25, mRNA (cDNA clone
MGC:87799 IMAGE:6141272), complete cds.
ACCESSION BC084579
VERSION BC084579.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5266)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5266)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-OCT-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: i Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56549655.
FEATURES Location/Qualifiers
source 1..5266
/db_xref="H-InvDB:HIT000266087"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87799 IMAGE:6141272"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5266
/gene="C18orf25"
/gene_synonym="ARKL1"
/gene_synonym="MGC12909"
/db_xref="GeneID:147339"
/db_xref="HGNC:HGNC:28172"
CDS 347..1378
/gene="C18orf25"
/gene_synonym="ARKL1"
/gene_synonym="MGC12909"
/codon_start=1
/product="chromosome 18 open reading frame 25"
/protein_id="AAH84579.1"
/db_xref="GeneID:147339"
/db_xref="HGNC:HGNC:28172"
/translation="MKMEEAVGKVEELIESEAPPKASEQETAKEEDGSVELESQVQKD
GVADSTVISSMPCLLMELRRDSSESQLASTESDKPTTGRVYESDSSNHCMLSPSSSGH
LADSDTLSSAEENEPSQAETAVEGDPSGVSGATVGRKSRRSRSESETSTMAAKKNRQS
SDKQNGRVAKVKGHRSQKHKERIRLLRQKREAAARKKYNLLQDSSTSDSDLTCDSSTS
SSDDDEEVSGSSKTITAEIPGHLDPGFLASDKTSAGNAPLNEEINIASSDSEVEIVGV
QEHARCVHPRGGVIQSVSSWKHGSGTQYVSTRQTQSWTAVTPQQTWASPAEVVDLTLD
EDSRRKYLL"
BASE COUNT 1418 a 1031 c 1117 g 1700 t
ORIGIN
1 gcctctggtg acattttctt gggggcggcg cggacgactg aagggacttg gtggtagagg
61 ctgcggaggc ggcgaggttc actttggtcc cggcaggggc tctgaccgcg acccactgct
121 cgctgccggg gcttgttccg aggaggagaa ggcggcggtg gccgtcgcgt cctgagtcac
181 cgcagcgggg aagagtcccg gccgggggtt cggtgcgtgc cccgacgggg cgcgcgcgaa
241 gcgagcgaga aggcacggag tcgccgcgga gcgcaggttg ctttcctgtt gcatctttga
301 tatcatttcc tagactctac tccatgattt aacttgaaat tctgaaatga agatggagga
361 ggcagtggga aaagttgaag aactcattga gtccgaagcc ccaccaaaag catctgaaca
421 agagacagcc aaggaggaag atggatctgt agaactggaa tctcaagttc agaaagatgg
481 tgtagcggat tctacagtta tttcttcaat gccctgcttg ttgatggaac tgagaaggga
541 ctcttctgag tctcagttag catccacaga gagtgacaag cctacaactg gccgagttta
601 tgagagtgac tcctctaatc actgcatgct ttccccttcc tctagtggtc acctggctga
661 ttcagatacg ttgtcttccg cagaagagaa tgaaccctct caggcagaaa cggcggtaga
721 aggagaccct tcaggagtgt ctggtgccac agttgggcgc aagtctaggc ggtcccgatc
781 tgaaagtgaa acttccacta tggctgccaa gaaaaaccgg caatccagtg ataaacagaa
841 tggccgagtc gccaaggtta aaggtcatcg gagccaaaag cacaaggaga ggatcaggct
901 actgaggcag aaacgggagg ctgctgcaag gaagaaatat aacctgctgc aggacagtag
961 taccagtgat agtgacctga cttgtgactc aagcacgagc tcatcagatg atgatgaaga
1021 ggtttcaggg agcagcaaga caatcactgc agagatacca ggtcatttgg atccaggatt
1081 cctagcaagt gacaaaacat ctgctggcaa tgcgccactc aatgaagaaa ttaacattgc
1141 gtcttcagat agtgaagtag agattgtggg agttcaggaa catgcaaggt gtgttcatcc
1201 tcgaggtggt gtgattcaga gtgtttcttc atggaagcat ggctcgggca cgcagtatgt
1261 tagcaccagg caaacacagt catggactgc tgtgactccc cagcagactt gggcttcacc
1321 agcagaagtt gttgacctta ccttggatga ggatagcagg cgtaaatacc tactgtaata
1381 caatgtcact gtgtttcctc tgcactgttc ccttccactt cctcatcctc tttgtgacat
1441 ggaagttcat tgtcatagct tcagcttcag aagctgtttg tggcatttgt aggattcaaa
1501 ctcatggaaa attccctcct cttcccccct ttttttaatt aaaagaagtc acttaggaaa
1561 ataacatcac agagaaaaga atttcttctt tccctcagta ggaatataga atgatgaatt
1621 ttattagaca actttatttt acttggtgac ttttaatctt aggaaactct gccttccttt
1681 tagaataata ttttaattgc caggcaaaat gggtttcagt ttttgaatct tgtatttatt
1741 tttctgtata atgaaattaa tatctagact tgaccactat ctgcccaccc cattggcact
1801 cccatcttag ggcattcgca cacatatcag tcaggtcctt attggcacct agtagggcat
1861 gtgcatttaa aaacttgtcc ttaaaaatag aagacagaag tattaaaatt atattttctt
1921 ttattttaag tcttctgcct tctttcttga gtttgctcct gttgacttcc cacagcacag
1981 ataattgaca tgtccctttg tgtttataaa tactactaag gtgacctagt tggtctcctt
2041 gttgcaataa ttaataactc tttgcaaaat gtctcctttg agacagaggc atttcagata
2101 gatacccacc atgaggtgtt ctgggcatct gaaggtatta agagttcagt gggaaaaatc
2161 tctctccctc gccttttttt ctggtttagt caaataacgg gtgaacatag aaacctgttg
2221 ggaagaatat gaaaagttat gtggcctctg aggctacagg cacttgcaaa tccgagagga
2281 aagtgccgtc tttcatagct ggtctctgta taaagagtat gtagcaacaa ctgtttaaca
2341 tccgagatga agtggacctg atgctgtatt tgggtatttt tctcctctac cttctaaaaa
2401 aaaataccat aagaagaatc agaatgtacc ataagaaatc agatgcaaag cacgttaatg
2461 tcattggtaa gttttacaga gtctaaaagg attagcaaag ggtagcagga gcttcaggga
2521 attcaggaaa tgccagcatt ggaattttgt ggcattagtt actaccttct ttcttataat
2581 atgttgtagt tactagtgaa ttcttttaaa agcaatacct agagttagaa cactatgtac
2641 tctgcacagg gggagtgaag taatattggc ttagaagact tcaccttttg tttttcatgg
2701 tttttgtttg ttttgttttt gtttttgttt ccattgttac cgttgctatg gccctttgcc
2761 tgtctctcca aatcccctgg tggctcttct tatcattaag agtatgagaa ttaccatttt
2821 cagttttcat tcagcaaaca aatttaccaa gtgcctgctc tgcataaggc actgtgaggg
2881 gtgggtgctc tgggggctgc tgagatgaat gacacacacc gcacatacac atgcatcagt
2941 gtctgcctgc ctccccctgc ccagcctgtt cttcttcttc ttcttcatct tcttcttttt
3001 tttttttttt ttgtaaagac aagtttattc cgttacgctt cttgttgcat tagttctcct
3061 gtatgttcct tcttccacag gaatgtattt gtgcatatgt tctttgatct tctctgatag
3121 catctcattt gatctggcct ctgtccttca tcagctttta tttcttttat ttttattttc
3181 tgcttttgtt agataaggct tttaagatca tgaacctcca gctttagcaa ctcatatatc
3241 agtctctcac atggccacaa ttctagacaa aaaccaaaga cccagaggcc agagcaaagt
3301 aggaccttct gaagattcac tactggtgta tccttggacc ttcacatttt actttaggat
3361 taatttattg aattttactg ttacacatag tttttgactt gaattaaaat actagacaaa
3421 gcagaaatgt acaatttctg gtctgtgttt tccaggaaat tcatagagaa gagaatagag
3481 tggatgatgt gacttgaagt ttttgcagct tattttttcc cccactgtca gggcagaggc
3541 ccattttaat gaatgttttt gcatgatctg ttcttatgtt aaccacatat tttttttccc
3601 aattccacac agcaggtgat ttcccgtgtg acatattttc ttcatagtcc ctaatatagt
3661 catcctctac agagttggcg tgctgtgtgc actgtggatt aggaacagag tggagctgta
3721 accagtctct tctctggccc tttgccttta tgtcagcttg cacttttgcc tcacttattc
3781 ctagtccagc tcccaactgc ctgacttaat ggaccagaac ggagatttgg atattaagat
3841 gcagaggggg aggggaaggg atggtagaga tagacagctc tcccctacta ggttgggtca
3901 tttcccctaa taactataga gagactgtgt ttcaactctc ttttcattgc tgtttccaga
3961 agagtggaga gtgtggtttc aggcaaggta ttaatgatac aattccgtgg tatacagctc
4021 ctgtgacatt tgaagtttct tttaaagggg tttttcttaa tcaaagtttt acatttggta
4081 gcataagtag ttctttatgc ttcacaggac attgtcttca gagtaatctc tctgctaaaa
4141 tggtacatgt gggtcacaga ttggtttatg ccactgactt ggttaagttt ccaccccctc
4201 caatttgtga aaaacagagc agtgacctag ctgttgcatc atcatgactg ccatgtaacc
4261 tgtaattgct gctctccagt aattgtcttt tcattttgat ggtaggggcc agactgaatg
4321 aataatgcat tcttttttat tcaatttatt tcattcagtt tattcttggc aatcaaacat
4381 cccataaact ttgcttttat tatcaattat gaatatgaat tgtctggttt actaatgtag
4441 tcatttgttc atctttactt ttagcagatt agcaccataa ctaacttaaa actaagtttg
4501 ttacctgctt tttatactgt cacaaaggtt tttgaaaaag ctggggacac tattttgtct
4561 tttagtttta aactttttta aggcaaagca agctccccaa tccctgttct ccaatatgtg
4621 tatgatgtga cttccatgta gatataaaaa tgattgtgcc ctaaccaaac ttcctcagat
4681 tgtgcactgt ttgtttaaaa taatcacgta ttttagtctg atgctgttgt attttttcat
4741 tttatttaaa acactactta cttcttattc tcttaacaaa ttttaaaagg tagtgatctt
4801 aaaaaaaaat cactggataa ctaggaaata tttttgttgt tgttttgttt cttaaagagt
4861 acagaggcca ttgaagcctt ttgctcctct taccgtgaaa atgaaatgtt agccattgta
4921 tggctaggaa ccaatgcact tggcagttaa tggatctgct gttgctggag tgtgagcgtg
4981 gacttgatgc agtgatgaca aatcattgct tagaattaat gttttcaaat gtgcaactct
5041 agtttttaaa acaaaatttg gttctttaca ttcattattt cgtttttgtt tccctttagt
5101 atttaatggt ctttggagaa aaaataataa aacagagtgt tatatatata ttttttggtg
5161 caagataaga ttttctgttt tttgaaataa gtctgtagca taaaggttaa actattttaa
5221 gacaaaataa aatagagtgt atttaaacaa aaaaaaaaaa aaaaaa
//