LOCUS BC080631 1392 bp mRNA linear HUM 18-MAR-2009
DEFINITION Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate
methyltransferase, mRNA (cDNA clone MGC:99627 IMAGE:6536541),
complete cds.
ACCESSION BC080631
VERSION BC080631.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1392)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1392)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-AUG-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 59 Row: m Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56699398
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1392
/db_xref="H-InvDB:HIT000265895"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:99627 IMAGE:6536541"
/tissue_type="Lung, epidermoid carcinoma"
/clone_lib="NIH_MGC_101"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene 1..1392
/gene="TRMU"
/gene_synonym="MTO2"
/gene_synonym="MTU1"
/gene_synonym="TRMT1"
/gene_synonym="TRNT1"
/db_xref="GeneID:55687"
/db_xref="HGNC:HGNC:25481"
/db_xref="MIM:610230"
CDS 323..991
/gene="TRMU"
/gene_synonym="MTO2"
/gene_synonym="MTU1"
/gene_synonym="TRMT1"
/gene_synonym="TRNT1"
/codon_start=1
/product="TRMU protein"
/protein_id="AAH80631.1"
/db_xref="GeneID:55687"
/db_xref="HGNC:HGNC:25481"
/db_xref="MIM:610230"
/translation="MKKSLSRSTLRSPNGFSEIGLKLEMVSQDALRRTIFPLGGLTKE
FVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTH
KGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIA
EEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQS
WDGH"
BASE COUNT 333 a 338 c 444 g 277 t
ORIGIN
1 agctggcgaa gttgggcgac tggcggatgc aggccttgcg gcacgtcgtg tgctccctgt
61 ccggcggcgt ggacagcgcc gtggccgcgc tgctgctgag gcggagaggt taccaggtga
121 caggggtgtt tatgaagaac tgggactcac tggatgaaca tggggtctgt actgccgaca
181 aagactgtga agatgcttac agagtttgcc agatcttaga catccctttc catcaagtgt
241 cctacgtaaa ggagtattgg aatgatgtgt tcaggggcag atgccattgc cacaggtcac
301 tatgcaagaa cttccctgga agatgaagaa gtctttgagc agaagcacgt taagaagccc
361 gaatggcttt tcagaaatcg gtttgaagtt agaaatggtt tcccaggatg ccctgaggag
421 aaccatcttc cctctggggg gattaacgaa agagtttgta aagaaaatcg ctgctgagaa
481 tagacttcat catgtgcttc agaagaaaga gagcatgggc atgtgtttca tcgggaagag
541 gaattttgaa catttccttc ttcagtatct gcagcctcga cctggtcact ttatttccat
601 agaagacaat aaggttctgg gaacacataa aggttggttc ctgtatacct tgggccagag
661 agcaaacata ggtggcctga gagagccctg gtacgtggtg gagaaggaca gcgtcaaggg
721 tgacgtgttt gtggcccccc ggacagacca cccagccctg tacagggacc tgctgaggac
781 cagccgcgtg cactggattg cggaggagcc tcccgcagca ctggtccggg acaagatgat
841 ggagtgccac ttccgattcc gccaccagat ggcactagtt tgctgtgttc tacaaggggg
901 acgagtgcct gggcagcggg aagatcctgc ggctggggcc gtctgcctac acgctccaga
961 agggccagcg cagagctggg atggccactg agagccccag tgacagccca gaagatggtc
1021 caggcctgag tcccttgctc tgacagagat ggatctgcta gaaggaacct ggagagcagg
1081 acccatggct gggcggctgg tgagcagtcc aggtgcccaa gggccagctt gctgctgccc
1141 aaagcagagg aagccgggct ggctgagggt ccgaaaagcc tgcaggggcc cggcgagccc
1201 caggaagagc ctcagctcca ggctggggct ctggctgctg gagcatctgc tggctggtgg
1261 ggtggcccga gttccccttc accgccccca gggagggttt cccacctcag agtacaccaa
1321 ggggacctgc agagggggct gtcgggacag cgtggaataa acattatttc aaggaaaaaa
1381 aaaaaaaaaa aa
//