LOCUS BC078149 2612 bp mRNA linear HUM 03-AUG-2004
DEFINITION Homo sapiens calpastatin, mRNA (cDNA clone IMAGE:6070772), partial
cds.
ACCESSION BC078149
VERSION BC078149.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2612)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2612)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: h Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 27765088.
FEATURES Location/Qualifiers
source 1..2612
/db_xref="H-InvDB:HIT000265708"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6070772"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2612
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/db_xref="GeneID:831"
/db_xref="MIM:114090"
CDS <1..1781
/gene="CAST"
/gene_synonym="BS-17"
/gene_synonym="MGC9402"
/codon_start=3
/product="CAST protein"
/protein_id="AAH78149.1"
/db_xref="GeneID:831"
/db_xref="MIM:114090"
/translation="DAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAI
SGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYT
GPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPKAQSAGTV
RSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLE
KCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEK
PSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLG
KKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESK
EQLPPILSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKD
LDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDD
NGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASS
SKAPKNGGKAKDSAKTTEETSKPKDD"
BASE COUNT 929 a 520 c 563 g 600 t
ORIGIN
1 acgatgctca caataaaaaa gcagtttcca gatcagctga acagcagcca tcagagaaat
61 caacagaacc aaagactaaa ccacaagaca tgatttctgc tggtggagag agtgttgctg
121 gtatcactgc aatatctggc aagccgggtg acaagaaaaa agaaaagaaa tcattaaccc
181 cagctgtgcc agttgaatct aaaccggata aaccatcggg aaagtcaggc atggatgctg
241 ctttggatga cttaatagat actttaggag gacctgaaga aactgaagaa gaaaatacaa
301 cgtatactgg accagaagtt tcagatccaa tgagttccac ctacatagag gaattgggta
361 aaagagaagt cacaattcct ccaaaatata gggaactatt ggctaaaaag gaagggatca
421 cagggcctcc tgcagactct tcgaaaccca tagggccaaa agctcagtca gcagggacag
481 tcagaagtgc tgctccaccc caagagaaga aaagaaaggt ggagaaggat acaatgagtg
541 atcaagcact cgaggctctg tcggcttcac tgggcacccg gcaagcagaa cctgagctcg
601 acctccgctc aattaaggaa gtcgatgagg caaaagctaa agaagaaaaa ctagagaagt
661 gtggtgagga tgatgaaaca atcccatctg agtacagatt aaaaccagcc acggataaag
721 atggaaaacc actattgcca gagcctgaag aaaaacccaa gcctcggagt gaatcagaac
781 tcattgatga actttcagaa gattttgacc ggtctgaatg taaagagaaa ccatctaagc
841 caactgaaaa gacagaagaa tctaaggccg ctgctccagc tcctgtgtcg gaggctgtgt
901 gtcggacctc catgtgtagt atacagtcag caccccctga gccggctacc ttgaagggca
961 cagtgccaga tgatgctgta gaagccttgg ctgatagcct ggggaaaaag gaagcagatc
1021 cagaagatgg aaaacctgtg atggataaag tcaaggagaa ggccaaagaa gaagaccgtg
1081 aaaagcttgg tgaaaaagaa gaaacaattc ctcctgatta tagattagaa gaggtcaagg
1141 ataaagatgg aaagccactc ctgccaaaag agtctaagga acagcttcca cccattttgt
1201 ctgaggactt ctctggtcca caaaatgctt catctcttaa atttgaagat gctaaacttg
1261 ctgctgccat ctctgaagtg gtttcccaaa ccccagcttc aacgacccaa gctggagccc
1321 caccccgtga tacctcgcag agtgacaaag acctcgatga tgccttggat aaactctctg
1381 acagtctagg acaaaggcag cctgacccag atgagaacaa accaatggaa gataaagtaa
1441 aggaaaaagc taaagctgaa catagagaca agcttggaga aagagatgac actatcccac
1501 ctgaatacag acatctcctg gatgataatg gacaggacaa accagtgaag ccacctacaa
1561 agaaatcaga ggattcaaag aaacctgcag atgaccaaga ccccattgat gctctctcag
1621 gagatctgga cagctgtccc tccactacag aaacctcaca gaacacagca aaggataagt
1681 gcaagaaggc tgcttccagc tccaaagcac ctaagaatgg aggtaaagcg aaggattcag
1741 caaagacaac agaggaaact tccaagccaa aagatgacta aagaaataca agttaaggta
1801 tctggtatct gcatgtaaaa tcttcagctg gtggatggtg acttttgaag aacaaaaggc
1861 tttggcaaca gaaaacaatt gttctgggtg atttctagaa tggtttttgt tgagtctctg
1921 aacatcctaa atattggttt gttattcttt tccagaaaga aaatgaattt gactggttca
1981 cctgtgtact gagtattgat aaactttgaa tttttttaat tgccttcaat tgggagagaa
2041 agctttatat ttgtaagaaa tatatttgat aaagtttctt aaagcaacac caaaaaaaca
2101 aaagaaaagc taagtgaatt tttgcacatt ctacacacag tgcctgtaaa tctcatttgt
2161 attttcagtt tgcccttaat tttttttgtt agtgtttaga aaacaatgtt ttaaacattc
2221 ttcagtgttc tgatttctta ttaccccctt tcctcttggg cttttgaact gtatttgatg
2281 ttgctttggg ataatgttta taagtcaaac ataagatatt gtacattggg cacatatctc
2341 ctcttgggct gctaataata aattaataac aggtaacctg gacaaaccag gaagcaccaa
2401 accccttttc agtttgaact cttctttgcc aggtgtgagg acttctgcat cttacagtca
2461 gcacagaaca cactgagact tgaatcaagt cagcaacaga gcaaaataaa ggttagataa
2521 gtccttgtgt agcaaatttc gagcataaga aataaaatct aattaattct tagggtaaaa
2581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
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