LOCUS BC077725 1833 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens platelet-derived growth factor beta polypeptide
(simian sarcoma viral (v-sis) oncogene homolog), mRNA (cDNA clone
MGC:78575 IMAGE:6379783), complete cds.
ACCESSION BC077725
VERSION BC077725.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1833)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1833)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: n Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505680.
FEATURES Location/Qualifiers
source 1..1833
/db_xref="H-InvDB:HIT000265689"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:78575 IMAGE:6379783"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_42"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1833
/gene="PDGFB"
/gene_synonym="c-sis"
/gene_synonym="PDGF2"
/gene_synonym="SSV"
/db_xref="GeneID:5155"
/db_xref="HGNC:HGNC:8800"
/db_xref="MIM:190040"
CDS 825..1550
/gene="PDGFB"
/gene_synonym="c-sis"
/gene_synonym="PDGF2"
/gene_synonym="SSV"
/codon_start=1
/product="platelet-derived growth factor beta polypeptide
(simian sarcoma viral (v-sis) oncogene homolog)"
/protein_id="AAH77725.1"
/db_xref="GeneID:5155"
/db_xref="HGNC:HGNC:8800"
/db_xref="MIM:190040"
/translation="MNRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDL
QRLLHGDPGEEDGAELDLNMTRSHSGGELESLARGRRSLGSLTIAEPAMIAECKTRTE
VFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVR
KKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRP
PKGKHRKFKHTHDKTALKETLGA"
BASE COUNT 363 a 610 c 577 g 283 t
ORIGIN
1 ctcgctgcaa agagaaaacc ggagcagccg cagctcgcag ctcgcagctc gcagcccgca
61 gcccgcagag gacgcccaga gcggcgagca ggcgggcaga cggaccgacg gactcgcgcc
121 gcgtccacct gtcggccggg cccagccgag cgcgcagcgg gcacgccgcg cgcgcggagc
181 agccgtgccc gccgcccggg ccccgcgcca gggcgcacac gctcccgccc ccctacccgg
241 cccgggcggg agtttgcacc tctccctgcc cgggtgctcg agctgccgtt gcaaagccaa
301 ctttggaaaa agttttttgg gggagacttg ggccttgagg tgcccagctc cgcgctttcc
361 gattttgggg gcctttccag aaaatgttgc aaaaaagcta agccggcggg cagaggaaaa
421 cgcctgtagc cggcgagtga agacgaacca tcgactgccg tgttcctttt cctcttggag
481 gttggagtcc cctgggcgcc cccacacggc tagacgcctc ggctggttcg cgacgcagcc
541 ccccggccgt ggatgctcac tcgggctcgg gatccgccca ggtagcggcc tcggacccag
601 gtcctgcgcc caggtcctcc cctgcccccc agcgacggag ccggggccgg gggcggcggc
661 gcccgggggc catgcgggtg agccgcggct gcagaggcct gagcgcctga tcgccgcgga
721 cccgagccga gcccaccccc ctccccagcc ccccaccctg gccgcggggg cggcgcgctc
781 gatctacgcg tccggggccc cgcggggccg ggcccggagt cggcatgaat cgctgctggg
841 cgctcttcct gtctctctgc tgctacctgc gtctggtcag cgccgagggg gaccccattc
901 ccgaggagct ttatgagatg ctgagtgacc actcgatccg ctcctttgat gatctccaac
961 gcctgctgca cggagacccc ggagaggaag atggggccga gttggacctg aacatgaccc
1021 gctcccactc tggaggcgag ctggagagct tggctcgtgg aagaaggagc ctgggttccc
1081 tgaccattgc tgagccggcc atgatcgccg agtgcaagac gcgcaccgag gtgttcgaga
1141 tctcccggcg cctcatagac cgcaccaacg ccaacttcct ggtgtggccg ccctgtgtgg
1201 aggtgcagcg ctgctccggc tgctgcaaca accgcaacgt gcagtgccgc cccacccagg
1261 tgcagctgcg acctgtccag gtgagaaaga tcgagattgt gcggaagaag ccaatcttta
1321 agaaggccac ggtgacgctg gaagaccacc tggcatgcaa gtgtgagaca gtggcagctg
1381 cacggcctgt gacccgaagc ccggggggtt cccaggagca gcgagccaaa acgccccaaa
1441 ctcgggtgac cattcggacg gtgcgagtcc gccggccccc caagggcaag caccggaaat
1501 tcaagcacac gcatgacaag acggcactga aggagaccct tggagcctag gggcatcggc
1561 aggagagtgt gtgggcaggg ttatttaata tggtatttgc tgtattgccc ccatggggtc
1621 cttggagtga taatattgtt tccctcgtcc gtctgtctcg atgcctgatt cggacggcca
1681 atggtgcttc ccccacccct ccacgtgtcc gtccaccctt ccatcagcgg gtctcctccc
1741 agcggcctcc ggcgtcttgc ccagcagctc aagaaaaaaa aaaaaaaaaa aaaaaaaaaa
1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//