LOCUS BC077721 4983 bp mRNA linear HUM 20-JUL-2004
DEFINITION Homo sapiens rho/rac guanine nucleotide exchange factor (GEF) 18,
mRNA (cDNA clone MGC:78478 IMAGE:5921159), complete cds.
ACCESSION BC077721
VERSION BC077721.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4983)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4983)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Lou Staudt
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: g Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14670376.
FEATURES Location/Qualifiers
source 1..4983
/db_xref="H-InvDB:HIT000265686"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:78478 IMAGE:5921159"
/tissue_type="Lymph, lymphoma"
/clone_lib="NIH_MGC_99"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene 1..4983
/gene="ARHGEF18"
/gene_synonym="KIAA0521"
/gene_synonym="MGC15913"
/db_xref="GeneID:23370"
CDS 275..3367
/gene="ARHGEF18"
/gene_synonym="KIAA0521"
/gene_synonym="MGC15913"
/codon_start=1
/product="ARHGEF18 protein"
/protein_id="AAH77721.1"
/db_xref="GeneID:23370"
/translation="MTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTAD
DSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAESWSLAVDAAYAKKQKREVVKR
QDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAIGRLFPCADDLLETHSHFL
ARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSGHNEAVSHY
KLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTE
DYEDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDM
LQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQK
LIVREVANEEKAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESCPDEEEGPF
SLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLEDLPQPR
GLFRGGDPSETLQGELILKSAMSEIEGIQSLICRRLGSANGQAEDGGSSTGPPRRAET
FAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVE
APGTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFR
LQSTRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGA
RLQEREGEARQLRERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLK
KQNTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDS
APTESRLAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGSQRWE
SSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADS
PSEGFSLKAGGTALLPGPPAPSPLPARWRRQHLSPESGRIHFPNRAPRRFTMNLRVRE
"
BASE COUNT 1111 a 1485 c 1535 g 852 t
ORIGIN
1 taccagtgtg aagcgcaggc tgagctgcct ccgcagtcga gtgaccaggc agaaggagaa
61 ggggaagagc ccagcacatc tgaaggacaa gggccaggat gcacgagaga ggcgggagtg
121 tgtcaatggg caccagctgt tgcaagggac cttctccggc ccctccagct gccccctgtg
181 tggcaaacct ttcttgagct cagcctcgct caaggagcac ccccggggca ccctcctgtc
241 cgatggcagc ccggccctgt ccaggaatgt cggtatgacg gtctctcaga aagggggtcc
301 ccagccaaca ccgagcccgg ctggccctgg gacgcaactc ggaccaatca caggagagat
361 ggatgaagcc gattctgcgt ttttaaaatt taagcagaca gctgatgact ctctgtccct
421 tacatctcca aacaccgagt ccatttttgt agaagatccc tacaccgcct cgctgaggag
481 tgagattgag tcagacggcc acgagtttga agctgagtcc tggagcctcg ccgtggatgc
541 agcctacgcc aagaagcaaa agagggaggt ggtgaaaaga caagatgtcc tttatgagct
601 gatgcagaca gaggtgcacc acgtgcggac gctcaagatc atgctgaagg tgtactccag
661 ggccctgcag gaggagctgc agttcagcag caaggccatt ggccgcctct tcccatgcgc
721 tgacgacctg ctggagacgc acagccactt cctcgctcgg ctcaaggagc gccgccagga
781 gtccctggag gagggcagtg accggaatta tgtcatccag aaaatcggcg acctcctggt
841 tcagcagttt tcaggtgaaa atggggagag aatgaaagaa aagtacggtg tgttttgtag
901 tggccacaat gaagctgtta gtcattacaa gttgctgctt cagcaaaaca agaaatttca
961 aaacttgatc aagaaaattg gcaacttctc catcgtgcgg cggcttggcg tgcaggagtg
1021 cattctcctg gttacacaac gcataaccaa atacccagtg ctggtggagc gcatcatcca
1081 gaacacggaa gctggcactg aggactatga agacctgacc caggccttga acctcatcaa
1141 agatatcatc tcacaagtgg acgccaaggt cagtgagtgt gagaagggcc agcgcctcag
1201 ggagatcgca gggaagatgg acctgaagtc ttccagcaaa ctcaagaacg ggctcacctt
1261 ccgcaaggaa gacatgcttc agcggcagct ccacctggag ggcatgctat gctggaagac
1321 cacatcaggg cgcttgaaag atatcctggc tatcctgctg accgacgtac ttttgctgct
1381 acaagaaaaa gatcagaaat acgtctttgc ttctgtggac tcaaagccac ccgtcatctc
1441 gttacaaaag ctcatcgtga gggaagtggc caacgaggag aaagcgatgt ttctgatcag
1501 cgcctccttg caagggccgg agatgtatga aatctacacg agctccaaag aggacaggaa
1561 cgcctggatg gcccacatcc aaagggctgt ggagagctgc cctgacgagg aggaggggcc
1621 cttcagcctg cccgaagagg aaaggaaggt ggtcgaggcc cgcgccacga gactccggga
1681 ctttcaagag cggttgagca tgaaagacca gctgatcgca cagagcctcc tagagaaaca
1741 gcagatctac ctggagatgg ccgagatggg cggcctcgaa gacctgcccc agccccgagg
1801 cctattccgt ggaggggacc catccgagac cctgcagggg gagctaattc tcaagtcggc
1861 catgagcgag atcgagggca tccagagcct gatctgcagg cggctgggca gcgccaacgg
1921 ccaggcggaa gacggaggca gctccacagg cccgcccagg agggctgaga ccttcgcggg
1981 ctacgactgc acaaacagcc ccaccaagaa tggcagtttc aagaagaaag tcagcagcac
2041 tgaccccagg ccccgagact ggcgaggccc cccaaacagc ccggacttga agctcagtga
2101 cagtgacatt cctgggagct ctgaggaatc gccgcaggtg gtggaggcgc caggcacgga
2161 atccgatccc cgtctgccca ccgtcctgga gtcggagctt gtccagcgga tccagacact
2221 gtcccagctg ctcctgaacc ttcaggcggt aatcgcccac caggacagct atgtggagac
2281 gcagcgggct gccatccagg agcgggagaa gcagttccgg ctgcagtcga cgcgtgggaa
2341 cctgctgctg gagcaggagc ggcaacgcaa cttcgagaag cagcgggagg agcgcgcggc
2401 cctggagaag ctgcagagcc agctgcggca cgagcagcag cgctgggagc gcgagcgcca
2461 gtggcagcac caggagctgg agcgtgcggg cgcgcggctg caggagcgcg agggcgaggc
2521 gcggcagcta cgcgagcggc tggagcagga gcgggccgag ctggagcgcc agcgccaggc
2581 ctaccagcac gacctggagc ggctgcgcga ggcccagcgt gccgtggagc gcgagcggga
2641 gcgcctggag ctgctgcgcc gcctcaagaa gcagaacacc gcgccaggcg cgctgccgcc
2701 cgacacactg gccgaggccc agcccccaag ccaccctccc agcttcaacg gggaagggct
2761 ggagggccct cgggtgagca tgctgccatc cggcgtgggg ccagagtacg cagagcgccc
2821 cgaggtggct cgccgggaca gcgcccccac cgagagccgg ctggccaaga gcgatgtgcc
2881 catccagctg ctcagcgcca ccaaccagtt ccagaggcag gcggccgtgc agcagcagat
2941 ccccaccaag ctggcggcct ccaccaaggg tggcaaggac aagggcggca agagcagggg
3001 ctctcagcgc tgggagagct cagcgtcctt cgacctgaag cagcagctgc tgctcaacaa
3061 gctcatgggg aaagatgaga gcacctcacg gaaccgccgc tcgctgagcc ctatcctgcc
3121 cggcagacac agtcctgcgc ccccaccaga ccctggcttc cccgccccga gcccaccgcc
3181 agctgacagc ccctccgagg gcttctctct caaggccggg ggcacagccc tcctgcccgg
3241 gcccccagct ccctcgccac tgccggccag gtggcgtcgg cagcatctgt ccccagaatc
3301 aggcagaatc cacttcccaa acagagcccc acgcaggttc accatgaacc tcagggtcag
3361 ggaatgagcc aggcacgggg gcatgggcag agagggccac ggggcagggc ccactgaggg
3421 aacatcagtg gccctccagt caggttctgt gggtttggaa gcccatcgtg aaaggggctg
3481 acctttgccc ctttttactt ggcattggtt ttgaaaccag ctgtttccca aactctgctt
3541 cccaagggca accgttgctg ttcacacgct cagcctgtct gggggagcgg gcctctagct
3601 tcagccaggg cgggtacaca ccctgggcac agggtcctca gcccccggga aatgagctcc
3661 cagggctggc gtcccacctt ccaggtgggg gctggcacat cacagactgt cgagagcgcc
3721 atgtcccagg gcatgcagag gatgcaccta gagacgttgc agcaagtgga caagtggccg
3781 ctgtgcgggc ccctcgcttg tagtgagctg ttgcagctta cggtccgttc cctggagggg
3841 tggaggaagg aggtgttggg cagcatcaaa ggtgctggga catcccaggg tggtgagatc
3901 catccacgat ccagctccgg tggagaaagg gcccatgtca agccttgttc tgcaccccaa
3961 gcattggtgg taggactggg tcctggctga tcgtccttgt tcccagtggg gtacatgtga
4021 gcccctgcca gggccaagtc cttctcccga acccagggtc ctgggaactg cagatcccgg
4081 ggggattcag cccttctccc actgtgctgg cagaggcact cctgtgacgc tgaatacagt
4141 gaacagggac attcccgcca ctcggggaca gatgggcaca agggagggga aactccatca
4201 ggaagtgctc ccctgggcag aggcgcccac tgggtgctgt gggctcagga gggggcgggg
4261 caggagctgg tgccaaccgg gaaccagagc cccacagcca tacagcccat tggtgacaag
4321 gtcctgagaa cacagtggcc aggtgtcccc aggctcctgg cccctccgac gacctcaact
4381 ctgcccagcc cggtccctgg ccatcagcga cgctgtccgc cccccgtcag atcccatgtg
4441 tgccatgttt atcatcagtg ttttgtattt ttgtactgag tatcggagca ctttacagaa
4501 gctgactgta cattcctgtt ctgttgtgaa gagaacattc ccagaccctg gcaccctcct
4561 gagccggcgt gtgccggtcc agccctccga gatgccacaa ttccttggat gggggagaag
4621 ttcaaggaat ttctgctcgg ccacgcggtg ggaaccccgc gtccccgcca tgtggcagag
4681 gggtctcagt cgtgctaggc atcgggcggc agcgccgaca gcccttccct cgccagtgcc
4741 cctcggccac tcctgggttg gagcccgatt ttatttgtaa agttgacagt cgagcaaatg
4801 ttcctatttt cgtgggatct gcacacgtct ttgtcagttg tggtcatgat cttagtcacc
4861 tgctaattat ttttacaatg attacaacat ttcctcactg cgggatattt ctgacccgct
4921 ttagaactta agacctgatt ctagcaataa acgtgtccga gatgaaaaaa aaaaaaaaaa
4981 aaa
//