LOCUS BC075822 3836 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphodiesterase 8A, mRNA (cDNA clone MGC:88492
IMAGE:6296489), complete cds.
ACCESSION BC075822
VERSION BC075822.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3836)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3836)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUL-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CGAP (Stanford)
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 58 Row: l Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47132535.
FEATURES Location/Qualifiers
source 1..3836
/db_xref="H-InvDB:HIT000265637"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:88492 IMAGE:6296489"
/tissue_type="Liver, hepatocellular carcinoma"
/clone_lib="NIH_MGC_100"
/lab_host="DH10B"
/note="Vector: pOTB7"
gene 1..3836
/gene="PDE8A"
/gene_synonym="HsT19550"
/db_xref="GeneID:5151"
/db_xref="HGNC:HGNC:8793"
/db_xref="MIM:602972"
CDS 81..2570
/gene="PDE8A"
/gene_synonym="HsT19550"
/codon_start=1
/product="phosphodiesterase 8A"
/protein_id="AAH75822.1"
/db_xref="GeneID:5151"
/db_xref="HGNC:HGNC:8793"
/db_xref="MIM:602972"
/translation="MGCAPSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQKTAALPR
TRGAGLLESELRDGSGKKVAVADVQFGPMRFHQDQLQVLLVFTKEDNQCNGFCRACEK
AGFKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQLDAEALCRSIRSSKLSENTVIVG
VVRRVDREELSVMPFISAGFTRRYVENPNIMACYNELLQLEFGEVRSQLKLRACNSVF
TALENSEDAIEITSEDRFIQYANPAFETTMGYQSGELIGKELGEVPINEKKADLLDTI
NSCIRIGKEWQGIYYAKKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKAEK
ISECVQSDTHTDNQTGKHKDRRKGSLDVKAVASRATEVSSQRRHSSMARIHSMTIEAP
ITKVINIINAAQESSPMPVTEALDRVLEILRTTELYSPQFGAKDDDPHANDLVGGLMS
DGLRRLSGNEYVLSTKNTQMVSSNIITPISLDDVPPRIARAMENEEYWDFDIFELEAA
THNRPLIYLGLKMFARFGICEFLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLH
ATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAV
LESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVN
SINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEW
AARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVD
LPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE"
BASE COUNT 1171 a 850 c 821 g 994 t
ORIGIN
1 gcggcccgga ggcgccgggt gggccgtttg ctgaccggat cgcggctacc cgccagcgtg
61 tccgcggcgc cgccgccagc atgggctgtg ccccgagcat ccacatttcc gagcgcctgg
121 tggccgagga cgcgcctagc cccgcggcac cgccgctgtc gtccggcggg ccgcgcctcc
181 cgcagggcca gaagacggcc gccttgcccc ggacccgcgg cgccggcctc ttggagtcgg
241 agcttcgcga cggcagcggc aagaaggtag cagtagctga tgtgcagttt ggccccatga
301 gatttcatca agatcaactt caggtacttt tagtgtttac caaagaagat aaccaatgta
361 atggattctg cagggcatgt gaaaaagcag ggtttaagtg tacagttacc aaggaggctc
421 aggctgtcct tgcctgtttc ctggacaaac atcatgacat tatcatcata gaccacagaa
481 atcctcgaca gctggatgca gaggcactgt gcaggtctat cagatcatca aaactctcag
541 aaaacacagt tattgttggt gtagtacgca gggtggatag agaagagttg tccgtaatgc
601 ctttcatttc tgctggattt acaaggaggt atgtagaaaa ccccaacatc atggcctgct
661 acaatgaact gctccagctg gagtttggag aggtgcgatc acaactgaaa ctcagggctt
721 gtaactcagt attcactgca ttagaaaaca gtgaagatgc aattgaaatt acaagcgaag
781 accgttttat acagtatgca aatcctgcat ttgaaacaac aatgggctat cagtcaggtg
841 aattaatagg gaaggagtta ggagaagtgc ctataaatga aaaaaaggct gacttgctcg
901 atactataaa ttcatgcatc aggataggca aggagtggca aggaatttac tatgccaaaa
961 agaaaaacgg agataatata caacaaaatg tgaagataat acctgtcatt ggacagggag
1021 gaaaaattag acactatgtg tccattatca gagtgtgcaa tggcaacaat aaggctgaga
1081 aaatatccga atgtgttcag tctgacactc atacagataa tcagacaggc aaacataaag
1141 acaggagaaa aggctcacta gacgtcaaag ctgttgcctc ccgtgcaact gaagtttcca
1201 gccagagacg acactcttcc atggcccgga tacattccat gacaattgag gcgcccatca
1261 ccaaggtaat caatattatc aatgctgccc aggaaagtag tcccatgcct gtgacagaag
1321 ccctagaccg tgtgctggaa attctaagaa ccactgagtt atattcacca cagtttggtg
1381 ctaaagatga tgatccccat gccaatgacc ttgttggggg cttaatgtct gatggtttgc
1441 gaagactatc agggaatgaa tatgttcttt caacaaaaaa cactcaaatg gtttcaagca
1501 atataatcac tcccatctcc cttgatgatg tcccaccacg gatagctcgg gccatggaaa
1561 atgaggaata ctgggacttt gatatttttg aactggaggc tgccacccac aataggcctt
1621 tgatttatct tggtctcaaa atgtttgctc gctttggaat ctgtgaattc ttacactgct
1681 ccgagtcaac gctaagatca tggttacaaa ttatcgaagc caattatcat tcctccaatc
1741 cctaccacaa ttctacacat tctgctgatg tgcttcatgc cactgcctat tttctctcca
1801 aggagaggat aaaggaaact ttagatccaa ttgatgaggt cgctgcactc atcgcagcca
1861 ccattcatga tgtggatcac cctgggagaa ccaactcctt cctgtgtaat gctggaagtg
1921 agctggccat tttgtacaat gacactgctg tgctggagag ccaccatgcg gccttggcct
1981 tccagctgac cactggagat gataaatgca atatatttaa aaacatggag aggaatgatt
2041 atcggacact gcgccagggg attatcgaca tggtcttagc cacagaaatg acaaagcact
2101 ttgagcatgt caacaaattt gtcaacagca tcaacaaacc cttggcaaca ctagaagaaa
2161 atggggaaac tgataaaaac caggaagtga taaacactat gcttaggact ccagagaacc
2221 ggaccctaat caaacgaatg ctgattaaat gtgctgatgt gtccaatccc tgccgacccc
2281 tgcagtactg catcgagtgg gctgcacgca tttcggaaga atatttttct cagactgatg
2341 aagagaagca gcagggctta cctgtggtga tgccagtgtt tgacagaaat acctgcagca
2401 tccccaaatc ccaaatctct ttcattgatt acttcatcac agacatgttt gatgcttggg
2461 atgcctttgt agacctgcct gatttaatgc agcatcttga caacaacttt aaatactgga
2521 aaggactgga cgaaatgaag ctgcggaacc tccgaccacc tcctgaatag tgggagacac
2581 cacccagagc cctgaagctt tgttccttcg gtcatttgga attcctgagg gcagccagag
2641 ctccttggtc ctttcagtac taggcagaac agcccccgat ctgcatagcc tgtgaaagcc
2701 cacggggaca tcagtaacct tctgcagcca ccatccaatg ccattactgt caagtgagac
2761 ttggccactg tagcctgggc ctgctgcagg agctcttcag aaaggcacat gaggaccacg
2821 gtttgcctca gtttctggta aaacacaagg tctggagtgc ccctgcaaag ggtattgatg
2881 gacttcctgc cagtgacaga gcatgtctat tgcaaacaat tctctcagtt acgttcagca
2941 cttaagaacg gctaatggca ataggatctt tagcaacttt ttcacatcat agaaggtgca
3001 atcgctcact tgggaacact actgagagtg acttctcttt taaaattgag tagcagatga
3061 aaaattaaaa tttgaacttg attattaata tcaattaaaa tgttttattt attttattaa
3121 aagctcaata ttttctatga attcaaaaat acttcagagc caaagccaac ttcaaatacc
3181 gtgaccaaat ttacatgatt catattcatt atgcattact tggtatacag acttattttc
3241 ataatgcaaa ttaataaaat gacactttta ctgcactata gaaatattca tgtatgttaa
3301 acttttctga ttgaggctaa ctggaaaaag ctggggtcgt attctaagtg ctaaagaagg
3361 ctgcttctac tgtatagaac ccagggctct gaaacagctc tagccgccta atgcacttca
3421 caggtaactc cccaaggtaa aactagactc tcttgttggt tcgcaaagaa aagttaggac
3481 ttaacacttt tttctaaaat tttataattc aatttccaaa agtctactct attttatact
3541 gtttctacaa aatattcctt ataaaaacaa agaacaaaaa ttgaatattt aatgaattga
3601 cattttataa ccaacctgtt tttatctacg gtgggaatct ttgatgccag aaatttataa
3661 agaggttctg tatcttcaca ccttgaataa gcataatacc ataaaaaatg acacttgaca
3721 tgtcaatgta tttgtcattt cattttaaac tcgtatttgt ggtttttttc ccagataaaa
3781 atgaaattaa accatttctt tttaagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//