LOCUS BC073961 3359 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol binding clathrin assembly
protein, mRNA (cDNA clone MGC:87981 IMAGE:6183858), complete cds.
ACCESSION BC073961
VERSION BC073961.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3359)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3359)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-JUN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. James R. Lupski
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 174 Row: m Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56788365.
FEATURES Location/Qualifiers
source 1..3359
/db_xref="H-InvDB:HIT000265183"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87981 IMAGE:6183858"
/tissue_type="Peripheral Nervous System, dorsal root
ganglion"
/clone_lib="Lupski_dorsal_root_ganglion"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3359
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
CDS 154..1986
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/codon_start=1
/product="phosphatidylinositol binding clathrin assembly
protein"
/protein_id="AAH73961.1"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
/translation="MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDY
LIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNT
LFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMN
TEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINL
LEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLD
ALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQAR
LKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPND
LLDLQQPTFHPSVHPMSTASQVASTWGGFTPSPVAQPHPSAGLNVDFESVFGNKSTNV
IVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTT
KNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMNGMHFPQYAPPVMAYPATTPTGM
IGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM"
BASE COUNT 1035 a 669 c 704 g 951 t
ORIGIN
1 cagcgcgcgg ccccgaaccg cgccccaggc cggcaggcgg ggaaggagcc ggtgggggta
61 gggggtgcgg tggggggtgg ggaccctccg gctcttgggg gtcccagtcc ccgccggctg
121 ctgagcgggt ggggtggtgg aggagctgca gagatgtccg gccagagcct gacggaccga
181 atcactgccg cccagcacag tgtcaccggc tctgccgtat ccaagacagt atgcaaggcc
241 acgacccacg agatcatggg gcccaagaaa aagcacctgg actacttaat tcagtgcaca
301 aatgagatga atgtgaacat cccacagttg gcagacagtt tatttgaaag aactactaat
361 agtagttggg tggtggtctt caaatctctc attacaactc atcatttgat ggtgtatgga
421 aatgagcgtt ttattcagta tttggcttca agaaacacgt tgtttaactt aagcaatttt
481 ttggataaaa gtggattgca aggatatgac atgtctacat ttattaggcg gtatagtaga
541 tatttaaatg agaaagcagt ttcatacaga caagttgcat ttgatttcac aaaagtgaag
601 agaggggctg atggagttat gagaacaatg aacacagaaa aactcctaaa aactgtacca
661 attattcaga atcagatgga tgcacttctt gattttaatg ttaatagcaa tgaacttaca
721 aatggggtaa taaatgctgc cttcatgctc ctgttcaaag atgccattag actgtttgca
781 gcatacaatg aaggaattat taatttgttg gaaaaatatt ttgatatgaa aaagaaccaa
841 tgcaaagaag gtcttgacat ctataagaag ttcctaacta ggatgacaag aatctcagag
901 ttcctcaaag ttgcagagca agttggaatt gacagaggtg atataccaga cctttcacag
961 gcccctagca gtcttcttga tgctttggaa caacatttag cttccttgga aggaaagaaa
1021 atcaaagatt ctacagctgc aagcagggca actacacttt ccaatgcagt gtcttccctg
1081 gcaagcactg gtctatctct gaccaaagtg gatgaaaggg aaaagcaggc agcattagag
1141 gaagaacagg cacgtttgaa agctttaaag gaacagcgcc taaaagaact tgcaaagaaa
1201 cctcatacct ctttaacaac tgcagcctct cctgtatcca cctcagcagg agggataatg
1261 actgcaccag ccattgacat attttctacc cctagttctt ctaacagcac atcaaagctg
1321 cccaatgatc tgcttgattt gcagcagcca acttttcacc catctgtaca tcctatgtca
1381 actgcttctc aggtagcaag tacatgggga ggattcactc cttctccagt tgcacagcca
1441 cacccttcag ctggccttaa tgttgacttt gaatctgtgt ttggaaataa atctacaaat
1501 gttattgtag attctggggg ctttgatgaa ctaggtggac ttctcaaacc aacagtggcc
1561 tctcagaacc agaaccttcc tgttgccaaa ctcccaccta gcaagttagt atctgatgac
1621 ttggattcat ctttagccaa ccttgtgggc aatcttggca tcggaaatgg aaccactaag
1681 aatgatgtaa attggagtca accaggtgaa aagaagttaa ctgggggatc taactggcaa
1741 ccaaaggttg caccaacaac cgcttggaat gctgcaacaa tgaatggcat gcattttcca
1801 caatacgcac cccctgtaat ggcctatcct gctactacac caacaggcat gataggatat
1861 ggaattcctc cacaaatggg aagtgttcct gtaatgacgc aaccaacctt aatatacagc
1921 cagcctgtca tgagacctcc aaaccccttt ggccctgtat caggagcaca gatacagttt
1981 atgtaacttg atggaagaaa atggaattac tccaaaaaga caagtgctca agcagcaaaa
2041 tccttacttc cagcaaaatc caaactgctg tctcttaaat ctcttaaact ctcttcttcc
2101 attagaatgc tacaagtaac tcagtgaagg cccatgaagg aaattgggac tagtttatag
2161 gagaacgtat caatacagtt tataaagcca agaattgcta tgatttaaga ctaagatctg
2221 tctttttggt gactaaccct tcaattcttt caactcctgt taatacccat aatcagtaac
2281 ctatcaagaa aagcccttat ttggaaagtg tgaaatttgt atttggaaaa gctgcctgga
2341 gagaagaact gtgtccttta ctgtatttca acaggactct tttgggggat caaaattaaa
2401 attcctaatt atgcattatc tttcttttct ccagtcctca caaatacaga aacaataact
2461 gaaattaact tttctttttt taaaaaaaat tatattcagt ttgcagtaga cattccttaa
2521 gtatttgtat ttatttatga ttatcaattt tacataacat taatattgta tcagacctcc
2581 ttatgaaaat gagtatggat gtgcacagta tgtttgattt ttatccacaa gaatgaatct
2641 gattcagaat gcttttctca gctgacatac agagcactaa atattttaag gcaagtccat
2701 aggtctgaat ctcttaagaa ttctcggcct ctgtgggatt tagggaagca ttataaatgc
2761 attaatcctt atagtcaatt ctgtgcctag gattttgcca gggaacagtt cactgactag
2821 gaaaagcact acattttaaa ttcagcatta gtgcattggg aaggatcttt actgctttgt
2881 gcttggcatg tcattatttt ccatttgaca ttagggcctt tccaaaatga atgtgaggaa
2941 ttgctttcac ttcaagactt tccttctttt cactaaaact ctagaaggtg ttacaagggg
3001 gagggaaggg gggcaaagtc cttgaacatt ttctttggct cgtgccatgt tatgatcata
3061 taccttttaa ataaggggaa atagtatctt taaagttaat gtctagccaa gagtttagta
3121 aacgaagaat taaactgcac tgttgatcgg tgctttgtgt aaatacatct ttaacatttg
3181 ggtggagagg ggccttaaga aggacagttc attgtaggaa agcaattctg tacatgagtt
3241 taagcattct tgttgcattg tctctgcaga ttctattttt gtttacaata ttaaaatgta
3301 tgttagcaaa atgggtggat tttcaaataa aatgcagctt ccacaaaaaa aaaaaaaaa
//