LOCUS BC070500 573 bp mRNA linear HUM 06-JUN-2006
DEFINITION Homo sapiens eukaryotic translation elongation factor 1 alpha 1,
mRNA (cDNA clone IMAGE:4809394), complete cds.
ACCESSION BC070500
VERSION BC070500.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 573)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 573)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 48 Row: g Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 25453469
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..573
/db_xref="H-InvDB:HIT000264373"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4809394"
/tissue_type="Lung, mucoepidermoid carcinoma"
/clone_lib="NIH_MGC_59"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..573
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="eEF1A-1"
/gene_synonym="EF-Tu"
/gene_synonym="GRAF-1EF"
/gene_synonym="HNGC:16303"
/gene_synonym="MGC16224"
/gene_synonym="PTI1"
/db_xref="GeneID:1915"
/db_xref="HGNC:HGNC:3189"
/db_xref="MIM:130590"
CDS 68..271
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="eEF1A-1"
/gene_synonym="EF-Tu"
/gene_synonym="GRAF-1EF"
/gene_synonym="HNGC:16303"
/gene_synonym="MGC16224"
/gene_synonym="PTI1"
/codon_start=1
/product="EEF1A1 protein"
/protein_id="AAH70500.1"
/db_xref="GeneID:1915"
/db_xref="HGNC:HGNC:3189"
/db_xref="MIM:130590"
/translation="MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK
EAAEMGRSPSLPRKLRRLNEYYP"
BASE COUNT 203 a 102 c 119 g 149 t
ORIGIN
1 ggggcttttt cgcaacgggt ttgccgccag aacacaggtg tcgtgaaaac tacccctaaa
61 agccaaaatg ggaaaggaaa agactcatat caacattgtc gtcattggac acgtagattc
121 gggcaagtcc accactactg gccatctgat ctataaatgc ggtggcatcg acaaaagaac
181 cattgaaaaa tttgagaagg aggctgctga gatgggaagg tcaccaagtc tgcccagaaa
241 gctcagaagg ctaaatgaat attatcccta atacctgcca ccccactctt aatcagtggt
301 ggaagaacgg tctcagaact gtttgtttca attggccatt taagtttagt agtaaaagac
361 tggttaatga taacaatgca tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac
421 cactttggtt ttcttttttg cgtgtggcag ttttaagtta ttagttttta aaatcagtac
481 tttttaatgg aaacaacttg accaaaaatt gtcacagaat tttgagaccc attaaaaaag
541 ttaaatgaca aaaaaaaaaa aaaaaaaaaa aaa
//