LOCUS BC069823 2126 bp mRNA linear HUM 08-MAR-2005
DEFINITION Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic
subunit, mRNA (cDNA clone MGC:97312 IMAGE:7262561), complete cds.
ACCESSION BC069823
VERSION BC069823.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2126)
AUTHORS Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD,
Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg
B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T,
Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin
GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL,
Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P,
Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy
SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH,
Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW,
Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J,
Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M,
Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW,
Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J,
Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U,
Smailus DE, Schnerch A, Schein JE, Jones SJ and Marra MA.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2126)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (29-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRBR Plate: 5 Row: h Column: 3.
FEATURES Location/Qualifiers
source 1..2126
/db_xref="H-InvDB:HIT000263973"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:97312 IMAGE:7262561"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_244"
/note="Vector: pPCR-Script Amp SK(+)"
gene 1..2126
/gene="PRKAA2"
/gene_synonym="AMPK"
/gene_synonym="AMPK2"
/gene_synonym="PRKAA"
/db_xref="GeneID:5563"
/db_xref="MIM:600497"
CDS 45..1703
/gene="PRKAA2"
/gene_synonym="AMPK"
/gene_synonym="AMPK2"
/gene_synonym="PRKAA"
/codon_start=1
/product="AMP-activated protein kinase alpha 2 catalytic
subunit"
/protein_id="AAH69823.1"
/db_xref="GeneID:5563"
/db_xref="MIM:600497"
/translation="MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAV
KILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELF
DYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGL
SNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEH
VPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYL
FPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIM
NQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKP
KSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNY
VKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAE
SHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR"
BASE COUNT 614 a 426 c 464 g 622 t
ORIGIN
1 ttagcggagc ggcaggcggt ggagcgaggc cgcgcgcgcc gaagatggct gagaagcaga
61 agcacgacgg gcgggtgaag atcggacact acgtgctggg cgacacgctg ggcgtcggca
121 ccttcggcaa agtgaagatt ggagaacatc aattaacagg ccataaagtg gcagttaaaa
181 tcttaaatag acagaagatt cgcagtttag atgttgttgg aaaaataaaa cgagaaattc
241 aaaatctaaa actctttcgt catcctcata ttatcaaact ataccaggtg atcagcactc
301 caacagattt ttttatggta atggaatatg tgtctggagg tgaattattt gactacatct
361 gtaagcatgg acgggttgaa gagatggaag ccaggcggct ctttcagcag attctgtctg
421 ctgtggatta ctgtcatagg catatggttg ttcatcgaga cctgaaacca gagaatgtcc
481 tgttggatgc acacatgaat gccaagatag ccgatttcgg attatctaat atgatgtcag
541 atggtgaatt tctgagaact agttgcggat ctccaaatta tgcagcacct gaagtcatct
601 caggcagatt gtatgcaggt cctgaagttg atatctggag ctgtggtgtt atcttgtatg
661 ctcttctttg tggcaccctc ccatttgatg atgagcatgt acctacgtta tttaagaaga
721 tccgaggggg tgtcttttat atcccagaat atctcaatcg ttctgtcgcc actctcctga
781 tgcatatgct gcaggttgac ccactgaaac gagcaactat caaagacata agagagcatg
841 aatggtttaa acaagatttg cccagttact tatttcctga agacccttcc tatgatgcta
901 acgtcattga tgatgaggct gtgaaagaag tgtgtgaaaa atttgaatgt acagaatcag
961 aagtaatgaa cagtttatat agtggtgacc ctcaagacca gcttgcagtg gcttatcatc
1021 ttatcattga caatcggaga ataatgaacc aagccagtga gttctacctc gcctctagtc
1081 ctccatctgg ttcttttatg gatgatagtg ccatgcatat tcccccaggc ctgaaacctc
1141 atccagaaag gatgccacct cttatagcag acagccccaa agcaagatgt ccattggatg
1201 cactgaatac gactaagccc aaatctttag ctgtgaaaaa agccaagtgg catcttggaa
1261 tccgaagtca gagcaaaccg tatgacatta tggctgaagt ttaccgagct atgaagcagc
1321 tggattttga atggaaggta gtgaatgcat accatcttcg tgtaagaaga aaaaatccag
1381 tgactggcaa ttacgtgaaa atgagcttac aactttacct ggttgataac aggagctatc
1441 ttttggactt taaaagcatt gatgatgaag tagtggagca gagatctggt tcctcaacac
1501 ctcagcgttc ctgttctgct gctggcttac acagaccaag atcaagtttt gattccacaa
1561 ctgcagagag ccattcactt tctggctctc tcactggctc tttgaccgga agcacattgt
1621 cttcagtttc acctcgcctg ggcagtcaca ccatggattt ttttgaaatg tgtgccagtc
1681 tgattactac tttagcccgt tgatctgtct ctagtttctt tctgttattg cactatgaaa
1741 atcagttata ttctttaaat ttttatctta cttttggata atatccactg caatactaat
1801 tgagaaacat gaattatttc caggggcaca caatgctatt gaaattactg aaaacaaaat
1861 atctgacatc ttatttactt gtagaaatct gtaattctat tgtgcctatg ataaattcac
1921 ataggcaata tctttaatag gttaatatca atgaagattt ttaattacaa taatgagttc
1981 actacagacg attaacacac cacactggcg aaccatctca atgtaagggt ggtttggcaa
2041 cacctccttg ctttgctgtt tggtgtaggt aaatctagtt tacttcctaa atttcagtag
2101 gctttatgct gtgtttatcg cccaat
//