LOCUS BC069237 3244 bp mRNA linear HUM 27-APR-2004
DEFINITION Homo sapiens ubiquilin 2, mRNA (cDNA clone MGC:78469
IMAGE:4543266), complete cds.
ACCESSION BC069237
VERSION BC069237.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3244)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3244)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (26-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: c Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 16753206.
FEATURES Location/Qualifiers
source 1..3244
/db_xref="H-InvDB:HIT000263391"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:78469 IMAGE:4543266"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3244
/gene="UBQLN2"
/gene_synonym="CHAP1"
/gene_synonym="CHAP1/DSK2"
/gene_synonym="Dsk2"
/gene_synonym="HRIHFB2157"
/gene_synonym="LIC-2"
/gene_synonym="N4BP4"
/gene_synonym="PLIC-2"
/gene_synonym="PLIC2"
/db_xref="GeneID:29978"
/db_xref="MIM:300264"
CDS 153..2027
/gene="UBQLN2"
/gene_synonym="CHAP1"
/gene_synonym="CHAP1/DSK2"
/gene_synonym="Dsk2"
/gene_synonym="HRIHFB2157"
/gene_synonym="LIC-2"
/gene_synonym="N4BP4"
/gene_synonym="PLIC-2"
/gene_synonym="PLIC2"
/codon_start=1
/product="UBQLN2 protein"
/protein_id="AAH69237.1"
/db_xref="GeneID:29978"
/db_xref="MIM:300264"
/translation="MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKE
EFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVI
KSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLS
STNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRN
PEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQ
EPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTST
TTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYM
RSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPR
AMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFT
PIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQA
LAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS
"
misc_feature 285..461
/gene="UBQLN2"
/gene_synonym="CHAP1"
/gene_synonym="CHAP1/DSK2"
/gene_synonym="Dsk2"
/gene_synonym="HRIHFB2157"
/gene_synonym="LIC-2"
/gene_synonym="N4BP4"
/gene_synonym="PLIC-2"
/gene_synonym="PLIC2"
/note="ubiquitin; Region: Ubiquitin family. This family
contains a number of ubiquitin-like proteins: SUMO (smt3
homologue), Nedd8, Elongin B, Rub1"
/db_xref="CDD:pfam00240"
misc_feature 1899..2012
/gene="UBQLN2"
/gene_synonym="CHAP1"
/gene_synonym="CHAP1/DSK2"
/gene_synonym="Dsk2"
/gene_synonym="HRIHFB2157"
/gene_synonym="LIC-2"
/gene_synonym="N4BP4"
/gene_synonym="PLIC-2"
/gene_synonym="PLIC2"
/note="UBA; Region: Ubiquitin Associated domain. The UBA
domain is a commonly occurring sequence motif in some
members of the ubiquitination pathway, UV excision repair
proteins, and certain protein kinases. Although its
specific role is so far unknown, it has been suggested
that UBA domains are involved in conferring protein target
specificity. The domain, a compact three helix bundle, has
a conserved GFP-loop and the proline is thought to be
critical for binding. The UBA domain is distinct from the
conserved three helical domain seen in the N-terminus of
EF-TS and eukaryotic NAC proteins"
/db_xref="CDD:cd00194"
BASE COUNT 879 a 831 c 698 g 836 t
ORIGIN
1 agagttgctg ggagtgcgcg cggtcggatc acaaggcggc ggcggaggag gcccagcccg
61 ctgcggcggt gcctccttcc ttcctccttc cctcgcgctc tctctttcgc ccgcccgcgc
121 cttccctgcc cgcctgcgtc accgcggccg ccatggctga gaatggcgag agcagcggcc
181 ccccgcgccc ctcccgcggc cctgctgcgg cccaaggctc ggctgctgcc ccggctgagc
241 ctaaaatcat caaagtcacg gtgaagactc ccaaagagaa agaggagttc gcggtgcccg
301 agaacagctc ggttcagcag tttaaggaag cgatttcgaa acgcttcaaa tcccaaaccg
361 atcagctagt gctgattttt gccggaaaaa tcttaaaaga tcaagatacc ttgatccagc
421 atggcatcca tgatgggctg actgttcacc ttgtcatcaa aagccagaac cgacctcagg
481 gccagtccac gcagcctagc aatgccgcgg gaactaacac tacctcggcg tcgactccca
541 ggagtaactc cacacctatt tccacaaata gcaacccgtt tgggttgggg agcctgggag
601 gacttgcagg ccttagcagc ctgggcttga gctcgaccaa cttctctgag ctccagagcc
661 agatgcagca gcagcttatg gccagccctg agatgatgat ccaaataatg gaaaatccct
721 ttgttcagag catgctttcg aatcccgatc tgatgaggca gctcattatg gctaatccac
781 agatgcagca attgattcag agaaacccag aaatcagtca cctgctcaac aacccagaca
841 taatgaggca gacactcgaa attgccagga atccagccat gatgcaagag atgatgagaa
901 atcaagacct ggctcttagc aatctagaaa gcatcccagg tggctataat gctttacggc
961 gcatgtacac tgacattcaa gagccgatgc tgaatgccgc acaagagcag tttgggggta
1021 atccatttgc ctccgtgggg agtagttcct cctctgggga aggtacgcag ccttcccgca
1081 cagaaaatcg cgatccacta cccaatccat gggcaccacc gccagctacc cagagttctg
1141 caactaccag cacgaccaca agcactggta gtgggtctgg caatagttcc agcaatgcta
1201 ctgggaacac cgttgctgcc gctaattatg tcgccagcat ctttagtacc ccaggcatgc
1261 agagcctgct gcaacagata actgaaaacc cccagctgat tcagaatatg ctgtcggcgc
1321 cctacatgag aagcatgatg cagtcgctga gccagaatcc agatttggct gcacagatga
1381 tgctgaatag cccgctgttt actgcaaatc ctcagctgca ggagcagatg cggccacagc
1441 tcccagcctt cctgcagcag atgcagaatc cagacacact atcagccatg tcaaacccaa
1501 gagcaatgca ggctttaatg cagatccagc aggggctaca gacattagcc actgaagcac
1561 ctggcctgat tccgagcttc actccaggtg tgggggtggg ggtgctggga accgctatag
1621 gccctgtagg cccagtcacc cccataggcc ccataggccc tatagtccct tttaccccca
1681 taggccccat tgggcccata ggacccactg gccctgcagc cccccctggc tccaccggct
1741 ctggtggccc cacggggcct actgtgtcca gcgctgcacc tagtgaaacc acgagtccta
1801 catcagaatc tggacccaac cagcagttca ttcagcaaat ggtgcaggcc ctggctggag
1861 caaatgctcc acagctgccg aatccagaag tcagatttca gcaacaactg gaacagctca
1921 acgcaatggg gttcttaaac cgtgaagcaa acttgcaggc cctaatagca acaggaggcg
1981 acatcaatgc agccattgaa aggctgctgg gctcccagcc atcgtaatca catttctgta
2041 cctggaaaaa aaatgtatct tatttttgat aatggctctt aaatctttaa acacacacac
2101 aaaatcgttc tttactttca ttttgattct tttaaatctg tctagttgta agtctaatat
2161 gatgcatttt aagatggagt ccctccctcc tacttccctc actccctttc tcctttgctt
2221 atttttccta ccttcccttc ctcttgtctc cccactccct ccctctttgt ttccttcctt
2281 ccttatttcc tttagtttcc ttccttagcc gttttgagtg gtgggaatca atgctgtttc
2341 actcaaaagt gttgcatgca aacacttctc tttattctgc atttattgtg atttttggaa
2401 acaggtatca accttcacag ttgggtgaac aagtgttgtc ctacagatgt ccaatttatt
2461 tgcattttta aacattagcc tatgatagta atttaatgta gaatgaagat attaaaaaca
2521 gaagcaaatt atttgaagct ctctaatttg tggtacgata ttgcttattg tgactttggc
2581 atgtattttt gctagcaaaa tgctgtaaga tttataccat tgatcttttt tgctatattt
2641 gtatacagta cagtaagcac aattggcact gtacatctaa aaatattaca gtagaatctg
2701 agtgtaatat gtgtaaccaa aatgagaaag aatacaagaa atgtttctgg agctagttat
2761 gtctcacaat tttgtagaat cttacagcat ctttgataaa cttctcagtg aaaatgttgg
2821 ctaggcaagt tcagttaaaa tatagtagaa atgtttatcc tggtatctct aagtatacat
2881 ttaattgtac agaaaattta cagtgtaaca ttgtgtcaac atttgcagat tgactgtata
2941 tgaccttaat ctttgtgcag cctgaaggat cagtgtagta atgccaggaa agtgcttttt
3001 acctaagact tccttctcag cttctcccat aaagagaccc taatatgcat tttgatttgt
3061 aattggaaat gtaactttca ctgaaagtgt catgtgatgt ttgcattact tttaactgct
3121 atgtataaag gaaagtgtgt cttttgactt catcagttat ttctcttgtg cacagagaaa
3181 aatgcattaa aaatgactaa aaaaaataaa aaattaaaaa atggaaaaaa aaaaaaaaaa
3241 aaaa
//