LOCUS BC068995 1637 bp mRNA linear HUM 26-APR-2004
DEFINITION Homo sapiens embryonic ectoderm development, mRNA (cDNA clone
MGC:78405 IMAGE:4637702), complete cds.
ACCESSION BC068995
VERSION BC068995.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1637)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1637)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (05-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: d Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24041019.
FEATURES Location/Qualifiers
source 1..1637
/db_xref="H-InvDB:HIT000263179"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:78405 IMAGE:4637702"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1637
/gene="EED"
/gene_synonym="HEED"
/gene_synonym="WAIT1"
/db_xref="GeneID:8726"
/db_xref="MIM:605984"
CDS 8..1408
/gene="EED"
/gene_synonym="HEED"
/gene_synonym="WAIT1"
/codon_start=1
/product="EED protein"
/protein_id="AAH68995.1"
/db_xref="GeneID:8726"
/db_xref="MIM:605984"
/translation="MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSI
ESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFN
WHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSH
PLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLW
NIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKE
SYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSGRAILHSHQQC
MRDPVSPNLRQHLSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMR
FSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSIL
IAVCDDASIWRWDRLR"
misc_feature 551..1396
/gene="EED"
/gene_synonym="HEED"
/gene_synonym="WAIT1"
/note="WD40; Region: WD40 domain, found in a number of
eukaryotic proteins that cover a wide variety of functions
including adaptor/regulatory modules in signal
transduction, pre-mRNA processing and cytoskeleton
assembly"
/db_xref="CDD:cd00200"
BASE COUNT 519 a 277 c 368 g 473 t
ORIGIN
1 gaggaatatg tccgagaggg aagtgtcgac tgcgccggcg ggaacagaca tgcctgcggc
61 caagaagcag aagctgagca gtgacgagaa cagcaatcca gacctctctg gagacgagaa
121 tgatgacgct gtcagtatag aaagtggtac aaacactgaa cgccctgata cacctacaaa
181 cacgccaaat gcacctggaa ggaaaagttg gggaaaggga aaatggaagt caaagaaatg
241 caaatattct ttcaaatgtg taaatagtct caaggaagat cataaccaac cattgtttgg
301 agttcagttt aactggcaca gtaaagaagg agatccatta gtgtttgcaa ctgtaggaag
361 caacagagtt accttgtatg aatgtcattc acaaggagaa atccggttgt tgcaatctta
421 cgtggatgct gatgctgatg aaaactttta cacttgtgca tggacctatg atagcaatac
481 gagccatcct ctgctggctg tagctggatc tagaggcata attaggataa taaatcctat
541 aacaatgcag tgtataaagc actatgttgg ccatggaaat gctatcaatg agctgaaatt
601 ccatccaaga gatccaaatc ttctcctgtc agtaagtaaa gatcatgctt tacgattatg
661 gaatatccag acggacactc tggtggcaat atttggaggc gtagaagggc acagagatga
721 agttctaagt gctgattatg atcttttggg tgaaaaaata atgtcctgtg gtatggatca
781 ttctcttaaa ctttggagga tcaattcaaa gagaatgatg aatgcaatta aggaatctta
841 tgattataat ccaaataaaa ctaacaggcc atttatttct cagaaaatcc attttcctga
901 tttttctacc agagacatac ataggaatta tgttgattgt gtgcgatggt taggcgattt
961 gatactttct aagagtggcc gtgccatttt acattcccac cagcaatgta tgagagatcc
1021 agtgtctccg aatcttcgcc agcatttgtc ttgtgaaaat gccattgtgt gctggaaacc
1081 tggcaagatg gaagatgata tagataaaat taaacccagt gaatctaatg tgactattct
1141 tgggcgattt gattacagcc agtgtgacat ttggtacatg aggttttcta tggatttctg
1201 gcaaaagatg cttgcattgg gcaatcaagt tggcaaactt tatgtttggg atttagaagt
1261 agaagatcct cataaagcca aatgtacaac actgactcat cataaatgtg gtgctgctat
1321 tcgacaaacc agttttagca gggatagcag cattcttata gctgtttgtg atgatgccag
1381 tatttggcgc tgggatcgac ttcgataaaa tacttttgcc taatcaaaat tagagtgtgt
1441 ttgttgtctg tgtaaaatag aattaatgta tcttgctagt aagggcacgt agagcattta
1501 gagttgtctt tcagcattca atcaggctga gctgaatgta gtgatgttta cattgtttac
1561 attctttgta ctgtcttcct gctcagactc tactgctttt aataaaaatt tatttttgta
1621 aaaaaaaaaa aaaaaaa
//