LOCUS BC068974 4168 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens splicing factor 3b, subunit 3, 130kDa, mRNA (cDNA
clone MGC:74943 IMAGE:6168677), complete cds.
ACCESSION BC068974
VERSION BC068974.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4168)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4168)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: o Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 54112120.
FEATURES Location/Qualifiers
source 1..4168
/db_xref="H-InvDB:HIT000263169"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74943 IMAGE:6168677"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4168
/gene="SF3B3"
/gene_synonym="KIAA0017"
/gene_synonym="RSE1"
/gene_synonym="SAP130"
/gene_synonym="SF3b130"
/gene_synonym="STAF130"
/db_xref="GeneID:23450"
/db_xref="HGNC:HGNC:10770"
/db_xref="MIM:605592"
CDS 149..3802
/gene="SF3B3"
/gene_synonym="KIAA0017"
/gene_synonym="RSE1"
/gene_synonym="SAP130"
/gene_synonym="SF3b130"
/gene_synonym="STAF130"
/codon_start=1
/product="splicing factor 3b, subunit 3, 130kDa"
/protein_id="AAH68974.1"
/db_xref="GeneID:23450"
/db_xref="HGNC:HGNC:10770"
/db_xref="MIM:605592"
/translation="MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPD
PNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKI
HQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEA
HKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLN
HVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRN
DLDDPERGMIFVCSATHKTKSMFFSLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVP
VAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPL
KNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVS
ELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSC
SLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYF
EMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDC
LQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPV
TGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLE
FASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLI
IIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPK
AGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLI
LNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDL
GKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYP
RWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQK
AEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEM
HLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEV
SKKLEDIRTRYAF"
BASE COUNT 1046 a 994 c 1055 g 1073 t
ORIGIN
1 tgttggagtt ggtggtggct taagttttga agggaggtag catccgttgg atatccacac
61 catccttctc gctgcaggct ttcttggact ccgtactgtt ggtgtaacca aggcctggag
121 gtctgggtgg ctcaggtttc ctgcagccat gtttctgtac aacttaacct tgcagagagc
181 cactggcatc agctttgcca ttcatggaaa cttttctgga accaaacaac aagaaattgt
241 tgtttcccgt gggaagatct tggagctgct tcgcccagac cccaacactg gcaaagtaca
301 taccctactc actgtggaag tattcggtgt tatccggtca ctcatggcct ttaggctgac
361 aggtggcacc aaagactaca ttgtagttgg cagtgactct ggtcgaattg ttattttgga
421 ataccagcca tctaagaata tgtttgagaa gattcaccaa gaaacctttg gcaagagtgg
481 atgccgtcgc atcgttcctg gccagttctt agctgtggat cccaaagggc gagccgttat
541 gattagtgcc attgagaaac agaaattggt gtatattttg aacagagatg ctgcagcccg
601 acttaccatt tcatctcccc tggaagccca caaagcaaac actttagtgt atcatgtagt
661 tggagtagat gtcggatttg aaaatccaat gtttgcttgt ctggaaatgg attatgagga
721 agcagacaat gatccaacag gggaagcagc agctaatacc cagcagacac ttactttcta
781 tgagctagac cttggtttaa atcatgtggt ccgaaaatac agtgaacctt tggaggaaca
841 cggcaacttc cttattacag ttccaggagg gtcagatggt ccaagtggag tactgatctg
901 ctctgaaaac tatattactt acaagaactt tggtgaccag ccagatatcc gctgtccaat
961 tcccaggagg cggaatgacc tggatgaccc tgaaagagga atgatttttg tctgctctgc
1021 aacccataaa accaaatcga tgttcttctc tttggctcaa actgagcagg gagatatctt
1081 taagatcact ttggagacag atgaagatat ggttactgag atccggctca aatattttga
1141 tactgtaccc gttgctgctg ccatgtgtgt gcttaaaaca gggttccttt ttgtagcatc
1201 agaatttgga aaccattact tatatcaaat tgcacatctt ggagatgatg atgaagaacc
1261 tgagttttca tcagccatgc ctctggaaga aggagacaca ttcttttttc agccaagacc
1321 acttaaaaac cttgtgctgg ttgatgagtt ggacagcctc tctcccattc tgttttgcca
1381 gatagctgat ctggccaatg aagatactcc acagttgtat gtggcctgtg gtaggggacc
1441 ccgatcatct ctgagagtcc taagacatgg acttgaggtg tcagaaatgg ctgtttctga
1501 gctacctggt aaccccaacg ctgtctggac agtgcgtcga cacattgaag atgagtttga
1561 tgcctacatt attgtgtctt tcgtgaatgc caccctagtg ttgtccattg gagaaactgt
1621 agaagaagtg actgactctg ggttcctggg gaccaccccg accttgtcct gctccttatt
1681 aggagatgat gccttggtgc aggtctatcc agatggcatt cggcacatac gagcagacaa
1741 gagagtcaat gagtggaaga cccctggaaa gaaaacaatt gtgaagtgtg cagtgaacca
1801 gcgacaagtg gtgattgccc tgacaggagg agagctggtc tatttcgaga tggatccttc
1861 aggacagctg aatgagtaca cagaacggaa ggagatgtca gcagatgtgg tgtgcatgag
1921 tctggccaat gtaccccctg gagagcagcg gtctcgcttc ctggctgtgg ggcttgtgga
1981 caacactgtc agaatcatct ccctggatcc ctcagactgt ttgcaacctc taagcatgca
2041 ggctctccca gcccagcctg agtccttgtg tatcgtggaa atgggtggga ctgagaagca
2101 ggatgagctg ggtgagaggg gctcgattgg cttcctatac ctgaatattg ggctacagaa
2161 cggtgtgctg ctgaggactg tcttggaccc tgtcactggg gatttgtctg atactcgcac
2221 tcggtacctg gggtcccgtc ctgtgaagct cttccgagtc cgaatgcaag gccaggaggc
2281 agtattggcc atgtcaagcc gctcatggtt gagctattct taccaatctc gcttccatct
2341 caccccactg tcttacgaga cactggaatt tgcatcgggt tttgcctcgg aacagtgtcc
2401 cgagggcatt gtggccatct ccaccaacac cctacggatt ttggcattag agaagctcgg
2461 tgctgtcttc aatcaagtag ccttcccact gcagtacaca cccaggaaat ttgtcatcca
2521 ccctgagagt aacaacctta ttatcattga aacggaccac aatgcctaca ctgaggccac
2581 gaaagctcag agaaagcagc agatggcaga ggaaatggtg gaagcagcag gggaggatga
2641 gcgggagctg gccgcagaga tggcagcagc attcctcaat gaaaacctcc ctgaatccat
2701 ctttggagct cccaaggctg gcaatgggca gtgggcctct gtgatccgag tgatgaatcc
2761 cattcaaggg aacacactgg accttgtcca gctggaacag aatgaggcag cttttagtgt
2821 ggctgtgtgc aggttttcca acactggtga agactggtat gtgctggtgg gtgtggccaa
2881 ggacctgata ctaaaccccc gatctgtggc agggggcttc gtctatactt acaagcttgt
2941 gaacaatggg gaaaaactgg agtttttgca caagactcct gtggaagagg tccctgctgc
3001 tattgcccca ttccagggga gggtgttgat tggtgtgggg aagctgttgc gtgtctatga
3061 cctgggaaag aagaagttac tccgaaaatg tgagaataag catattgcca attatatctc
3121 tgggatccag actatcggac atagggtaat tgtatctgat gtccaagaaa gtttcatctg
3181 ggttcgctac aagcgtaatg aaaaccagct tatcatcttt gctgatgata cctacccccg
3241 atgggtcact acagccagcc tcctggacta tgacactgtg gctggggcag acaagtttgg
3301 caacatatgt gtggtgaggc tcccacctaa caccaatgat gaagtagatg aggatcctac
3361 aggaaacaaa gccctgtggg accgtggctt gctcaatggg gcctcccaga aggcagaggt
3421 gatcatgaac taccatgtcg gggagacggt gctgtccttg cagaagacca cgctgatccc
3481 tggaggctca gaatcacttg tctataccac cttgtctgga ggaattggca tccttgtgcc
3541 attcacgtcc catgaggacc atgacttctt ccagcatgtg gaaatgcacc tgcggtctga
3601 acatccccct ctctgtgggc gggaccacct cagctttcgc tcctactact tccctgtgaa
3661 gaatgtgatt gatggagacc tctgtgagca gttcaattcc atggaaccca acaaacaaaa
3721 gaacgtctct gaagaactgg accgaacccc acccgaagtg tccaagaaac tcgaggatat
3781 ccggacccgc tacgccttct gagccctcct ttcccggtgg ggcttgccag agactgtgtg
3841 ttttgtttcc cccaccacca tcactgccac ctggcttctg ccatgtggca ggagggtgac
3901 tggataatta agactgcatt atgaaagtca acagctcttt cccctcagct cttctcctgg
3961 aatgactggc ttcccctcaa attggcactg agatttgcta cacttctccc cacctggtac
4021 atgatacatg accccaggtt ccagtgtaga acctgagtcc cccattcccc aaagccatcc
4081 ctgcattgat atgtcttgac tctcctgtct acttttgcac acacccttaa tttttaattg
4141 gttttcttgt aaaaaaaaaa aaaaaaaa
//