LOCUS BC068479 2730 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens transmembrane protein 214, mRNA (cDNA clone MGC:87131
IMAGE:4814193), complete cds.
ACCESSION BC068479
VERSION BC068479.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2730)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2730)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 167 Row: d Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 53793659
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..2730
/db_xref="H-InvDB:HIT000263036"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87131 IMAGE:4814193"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2730
/gene="TMEM214"
/db_xref="GeneID:54867"
/db_xref="HGNC:HGNC:25983"
CDS 63..2132
/gene="TMEM214"
/codon_start=1
/product="TMEM214 protein"
/protein_id="AAH68479.1"
/db_xref="GeneID:54867"
/db_xref="HGNC:HGNC:25983"
/translation="MATKTAGVGRWEVVKKGRRPGVGAGAGGRGGGRNRRALGEANGV
WKYDLTPAIQTTSTLYERGFENIMKRQNKEQVPPPAVEPKKPGNKKQPKKVATPHNQN
QKQGRFRSLEEALKALDVADLQKELDKSQSVFSGNQSIWLKDLASYLNYKLQAPLSEP
TLSQHTHDYPYSLVSRELRGIIRGLLAKAAGSLELFFDHCLFTMLQELDKTPGESLHG
YRICIQAILQDKPKIATANLGKFLELLRSHQSRPAKCLTIMWALGQAGFANLTEGLKV
WLGIMLPVLGIKSLSPFAITYLDRLLLMHPNLTKGFGMIGPKDFFPLLDFAYMPNNSL
TPSLQEQLCQLYPRLKVLAFGAKPDSTLHTYFPSFLSRATPSCPPEMKKELLSSLTEC
LTVDPLSASVWRQLYPKHLSQSSLLLEHLLSSWEQIPKKVQKSLQETIQSLKLTNQEL
LRKGSSNNQDVVTCDMACKGLLQQVQGPRLPWTRLLLLLLVFAVGFLCHDLRSHSSFQ
ASLTGRLLRSSGFLPASQQACAKLYSYSLQGYSWLGETLPLWGSHLLTVVRPSLQLAW
AHTNATVSFLSAHCASHLAWFGDSLTSLSQRLQIQLPDSVNQLLRYLRELPLLFHQNV
LLPLWHLLLEALAWAQEHCHEACRGEVTWDCMKTQLSEAVHWTWLCLQDITVAFLDWA
LALISQQ"
BASE COUNT 608 a 809 c 737 g 576 t
ORIGIN
1 gcggtgcgct cgcgccggac cggaaagccg gggaagtggc cgaggaggga gggctgcgag
61 ccatggcgac caagacggcg ggcgtggggc ggtgggaggt agtgaagaag ggtcggcggc
121 ctggggtcgg cgccggcgcc ggcggccgag gaggcggcag gaaccgcagg gcgctcgggg
181 aagcaaacgg agtgtggaaa tacgacctga cccctgcaat ccagaccaca agcacccttt
241 atgagcgggg ctttgagaat atcatgaagc ggcagaataa ggagcaggtc ccaccccctg
301 ctgtggaacc taagaaacca gggaacaaga agcagccaaa gaaggtggca actcctcaca
361 accaaaacca gaagcagggc cgcttccgca gcctggagga agcactgaaa gctctggatg
421 tggcagacct gcagaaggaa ctggacaaga gccagagtgt gttctctgga aaccaatcca
481 tatggttgaa ggacctggcc agctatctca actacaagct acaagctcct ctaagtgaac
541 ccacgctgag ccagcatact catgattatc cctacagcct ggtgagccgg gagctacgtg
601 ggatcatacg agggctgctg gcgaaggcag cggggtctct ggagctcttt tttgaccact
661 gtctgttcac catgttgcaa gagctggata agacaccagg ggagtcacta catggttacc
721 gcatctgtat ccaggccatc ctgcaagaca agcccaagat tgccacggca aacctaggca
781 agttcctgga actgctgagg tcccaccaga gccgaccagc aaagtgtctc accatcatgt
841 gggccctggg tcaagcaggt tttgccaacc tcaccgaggg actgaaagtg tggctgggga
901 tcatgctgcc tgtgctgggc atcaagtctc tgtctccctt tgccatcaca tacctggatc
961 ggctgctcct gatgcatccc aaccttacca agggcttcgg catgattggc cccaaggact
1021 tcttcccact tctggacttt gcctatatgc caaacaactc cctgacaccc agcctgcagg
1081 agcagctgtg tcagctctac ccccgactga aagtgctggc atttggagca aagccggatt
1141 ccaccctgca tacctacttc ccttctttcc tgtccagagc cacccctagc tgtccccctg
1201 agatgaagaa agagctcctg agcagcctga ctgagtgcct gacggtggac cccctcagtg
1261 ccagcgtctg gaggcagctg taccctaagc acctgtcaca gtccagcctt ctgctggagc
1321 acttgctcag ctcctgggag cagattccca agaaggtaca gaagtctttg caagaaacca
1381 ttcagtccct caagcttacc aaccaggagc tgctgaggaa gggtagcagt aacaaccagg
1441 atgtcgtcac ctgtgacatg gcctgcaagg gcctgttgca gcaggttcag ggtcctcggc
1501 tgccctggac gcggctcctc ctgttgctgc tggtcttcgc tgtaggcttc ctgtgccatg
1561 acctccggtc acacagctcc ttccaggcct cccttactgg ccggttgctt cgatcatctg
1621 gcttcttacc tgctagccaa caagcgtgtg ccaagctcta ctcctacagt ctgcaaggct
1681 acagctggct gggggagaca ctgccgctct ggggctccca cctgctcacc gtggtgcggc
1741 ccagcttgca gctggcctgg gctcacacca atgccacagt cagcttcctt tctgcccact
1801 gtgcctctca ccttgcgtgg tttggtgaca gtctcaccag tctctctcag aggctacaga
1861 tccagctccc cgattccgtg aatcagctac tccgctatct gagagagctg cccctgcttt
1921 tccaccagaa tgtgctgctg ccactgtggc acctcttgct tgaggccctg gcctgggccc
1981 aggagcactg ccatgaggca tgcagaggtg aggtgacctg ggactgcatg aagacacagc
2041 tcagtgaggc tgtccactgg acctggcttt gcctacagga cattacagtg gctttcttgg
2101 actgggcact tgccctgata tcccagcagt aggccctgcc ttcctggcca ctgatttctg
2161 catgggtaga ccatccaaga ctgcagcggg tagaaggtgg cagttcttca tgggagtctt
2221 tttaacttgg tgcctgagtt ctctcctaga ctttagcagc ctcctgttgc aattcttggt
2281 cacagctcta gccacagcag aaggaaaggg gcttccagaa gaatatagca ccgcattggg
2341 aaacagcagc ctcacctcca cctgaagcct gggtttggct gtcagtggac atggggagct
2401 ggatggaaat gcctctcact tcaaaatgcc cagcctgccc caaatgcctc taagcccctc
2461 cctgtcccct cccttgtagt cctacttctt ccaactttcc attccccatc atgctggggg
2521 tcttggtcac aaggctcagc ttctctccac tgtccatccc tcctatcatc tgtagagcag
2581 agcacaggca gttgtgtgcc ttgggcccag ggaaccctcc atcaacctga gacaggactc
2641 agtatatggt tcttgggtat gccctaccag gtggaataaa ggacacagat ttgatttcta
2701 aaaaaaaaaa aaaagaaaaa aaaaaaaaaa
//