LOCUS BC068476 3509 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens numb homolog (Drosophila), mRNA (cDNA clone MGC:87117
IMAGE:4822300), complete cds.
ACCESSION BC068476
VERSION BC068476.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3509)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3509)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 167 Row: f Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 54144623.
FEATURES Location/Qualifiers
source 1..3509
/db_xref="H-InvDB:HIT000263033"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:87117 IMAGE:4822300"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3509
/gene="NUMB"
/gene_synonym="S171"
/db_xref="GeneID:8650"
/db_xref="HGNC:HGNC:8060"
/db_xref="MIM:603728"
CDS 321..2099
/gene="NUMB"
/gene_synonym="S171"
/codon_start=1
/product="numb homolog (Drosophila)"
/protein_id="AAH68476.1"
/db_xref="GeneID:8650"
/db_xref="HGNC:HGNC:8060"
/db_xref="MIM:603728"
/translation="MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYL
GHVEVDESRGMHICEDAVKRLKATGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEK
VSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQK
REKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKKAETDKIVVGSS
VAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKM
SPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQITNAFSTPEDPF
SSAPMTKPVTVVAPQSPTFQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSV
RAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPAQ
SYPVANGMPYPAPNVPVVGITPSKMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEA
SSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALEN
KSKQRTNPSPTNPFSSDLQKTFEIEL"
BASE COUNT 1005 a 809 c 793 g 902 t
ORIGIN
1 gttgtcatgg gggaggtggt ggcgcttggt ggccactggc ggccgaggta gaggcagtgg
61 cgcttgagtt ggtcgggggc agcggcagat ttgaggctta agcaacttct tccggggaag
121 agtgccagtg cagccactgt tacaattcaa gatcttgatc tatatccata gattggaata
181 ttggtgggcc agcaatcctc agacgcctca cttaggacaa atgaggaaac tgaggcttgg
241 tgaagttacg aaacttgtcc aaaatcacac aacttgtaaa gggcacagcc aagattcaga
301 gccaggctgt aaaaattaaa atgaacaaat tacggcaaag ttttaggaga aagaaggatg
361 tttatgttcc agaggccagt cgtccacatc agtggcagac agatgaagaa ggcgttcgca
421 ccggaaaatg tagcttcccg gttaagtacc ttggccatgt agaagttgat gaatcaagag
481 gaatgcacat ctgtgaagat gctgtaaaaa gattgaaagc tactggaaag aaagcagtta
541 aagcagttct gtgggtctca gcagatggac tcagagttgt ggatgaaaaa actaaggacc
601 tcatagttga ccagacgata gagaaagttt ctttctgtgc cccagacagg aactttgata
661 gagccttttc ttacatatgc cgtgatggca ccactcgtcg ctggatctgt cactgcttca
721 tggctgtcaa ggacacaggt gaaaggttga gccatgcagt aggctgtgct tttgcagcct
781 gtttagagcg caagcagaag cgggagaagg aatgtggagt gactgctact tttgatgcta
841 gtcggaccac ttttacaaga gaaggatcat tccgtgtcac aacagccact gaacaagcag
901 aaagagagga gatcatgaaa caaatgcaag atgccaagaa agctgaaaca gataagatag
961 tcgttggttc atcagttgcc cctggcaaca ctgccccatc cccatcctct cccacctctc
1021 ctacttctga tgccacgacc tctctggaga tgaacaatcc tcatgccatc ccacgccggc
1081 atgctccaat tgaacagctt gctcgccaag gctctttccg aggttttcct gctcttagcc
1141 agaagatgtc accctttaaa cgccaactat ccctacgcat caatgagttg ccttccacta
1201 tgcagaggaa gactgatttc cccattaaaa atgcagtgcc agaagtagaa ggggaggcag
1261 agagcatcag ctccctgtgc tcacagatca ccaatgcctt cagcacacct gaggacccct
1321 tctcatctgc tccgatgacc aaaccagtga cagtggtggc accacaatct cctaccttcc
1381 aagggaccga gtggggtcaa tcttctggtg ctgcctctcc aggtctcttc caggccggtc
1441 atagacgtac tccctctgag gccgaccgat ggttagaaga ggtgtctaag agcgtccggg
1501 ctcagcagcc ccaggcctca gctgctcctc tgcagccagt tctccagcct cctccaccca
1561 ctgccatctc ccagccagca tcacctttcc aagggaatgc attcctcacc tctcagcctg
1621 tgccagtggg tgtggtccca gccctgcaac cagcctttgt ccctgcccag tcctatcctg
1681 tggccaatgg aatgccctat ccagccccta atgtgcctgt ggtgggcatc actccctcca
1741 agatggtggc caacgtattt ggcactgcag gccaccctca ggctgcccat ccccatcagt
1801 cacccagcct ggtcaggcag cagacattcc ctcactacga ggcaagcagt gctaccacca
1861 gtcccttctt taagcctcct gctcagcacc tcaacggttc tgcagctttc aatggtgtag
1921 atgatggcag gttggcctca gcagacaggc atacagaggt tcctacaggc acctgcccag
1981 tggatccttt tgaagcccag tgggctgcat tagaaaataa gtccaagcag cgtactaatc
2041 cctcccctac caaccctttc tccagtgact tacagaagac gtttgaaatt gaactttaag
2101 caatcattat ggctatgtat cttgtccata ccagacaggg agcagggggt agcggtcaaa
2161 ggagcaaaac agactttgtg tcctgattag tactcttttc actaatccca aaggtcccaa
2221 ggaacaagtc caggcccaga gtactgtgag gggtgatttt gaaagacatg ggaaaaagca
2281 ttcctagaga aaagctgcct tgcaattagg ctaaagaagt caaggaaatg ttgctttctg
2341 tactccctct tcccttaccc ccttacaaat ctctggcaac agagaggcaa agtatctgaa
2401 caagaatcta tattccaagc acatttactg aaatgtaaaa cacaacagga agcaaagcaa
2461 tctccctttg tttttcaggc cattcacctg cctcctgtca gtagtggcct gtattagaga
2521 tcaagaagag tggtttgtgc tcaggctggg gaacagagag gcacgctatg ctgccagaat
2581 tcccaggagg gcatatcagc aactgcccag cagagctata ttttggggga gaagttgagc
2641 ttccattttg agtaacagaa taaatattat atatatcaaa agccaaaatc tttattttta
2701 tgcatttaga atattttaaa tagttctcag atattaagaa gttgtatgag ttgtaagtaa
2761 tcttgccaaa ggtaaagggg ctagttgtaa gaaattgtac ataagattga tttatcattg
2821 atgcctactg aaataaaaag aggaaaggct ggaagctgca gacaggatcc ctagcttgtt
2881 ttctgtcagt cattcattgt aagtagcaca ttgcaacaac aatcatgctt atgaccaata
2941 cagtcactag gttgtagttt tttttaaata aaggaaaagc agtattgtcc tggttttaaa
3001 cctatgatgg aattctaatg tcattatttt aatggaatca atcgaaatat gctctataga
3061 gaatatatct tttatatatt gctgcagttt ccttatgtta atcctttaac actaaggtaa
3121 catgacataa tcataccata gaagggaaca caggttacca tattggtttg taatatgggt
3181 cttggtgggt tttgttttat cctttaaatt ttgttcccat gagttttgtg gggatgggga
3241 ttctggtttt attagctttg tgtgtgtcct cttcccccaa accccctttt ggtgagaaca
3301 tccccttgac agttgcagcc tcttgacctc ggataacaat aagagagctc atctcatttt
3361 tacttttgaa cgttggcctt acaatcaaat gtaagttata tatatttgta ctgatgaaaa
3421 tttataatct gctttaacaa aaataaatgt tcatggtaga aaaaaaaaaa aaaaaaaaaa
3481 aaaaaaaaaa aaagaaaaaa aaaaaaaaa
//