LOCUS BC068223 949 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens slingshot homolog 2 (Drosophila), mRNA (cDNA clone
IMAGE:2820595), complete cds.
ACCESSION BC068223
VERSION BC068223.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 949)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 949)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 54 Row: a Column: 1
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..949
/db_xref="H-InvDB:HIT000262978"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2820595"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..949
/gene="SSH2"
/gene_synonym="KIAA1725"
/gene_synonym="SSH-2"
/db_xref="GeneID:85464"
/db_xref="MIM:606779"
CDS 153..740
/gene="SSH2"
/gene_synonym="KIAA1725"
/gene_synonym="SSH-2"
/codon_start=1
/product="SSH2 protein"
/protein_id="AAH68223.1"
/db_xref="GeneID:85464"
/db_xref="MIM:606779"
/translation="MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTV
KGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQN
RTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLDGDGGFSV
STDNRVHIFKPVSVQAMWVDRDSRNKHCDVLLVEE"
BASE COUNT 264 a 250 c 210 g 225 t
ORIGIN
1 gcggagtctg cggccgttcc cgcggcctcc tcctcctccc cgttcccttc acccccaccc
61 cgcacccctt tccccatccc ggctccgtca ccctcccgtc ccccacactc aggacaagaa
121 tgccctgccc ggaacaaccc agcagcgcct agatggcttt ggtcacggtc cagcggtcac
181 ctacccccag caccacctcc agcccctgcg cctcggaggc agacagtggg gaggaagaat
241 gccggtcaca gcccaggagc atcagcgaga gctttctaac tgtcaaaggt gctgcccttt
301 ttctaccacg gggaaatggc tcatccacac caagaatcag ccacagacgg aacaagcatg
361 caggcgatct ccaacagcat ctccaagcaa tgttcatttt actccgccca gaagacaaca
421 tcaggctggc tgtaagactg gaaagtactt accagaatcg aacacgctat atggtagtgg
481 tttcaactaa tggtagacaa gacactgaag aaagcatcgt cctaggaatg gatttctcct
541 ctaatgacag tagcacttgt accatgggct tagttttgcc tctctggagc gacacgctaa
601 ttcatttgga tggtgatggt gggttcagtg tatcgacgga taacagagtt cacatattca
661 aacctgtatc tgtgcaggca atgtgggttg acagggattc aaggaacaaa cactgtgatg
721 tactattggt ggaagaatga actggagcag cctttctgga gagtgatttg ccaatatgcc
781 ttatcatttt gcatgatctt tgtcctagta actctatttc tatggattta ctctaagttt
841 gtaaacatgg atgtgtgcaa agattttagc tctaagaatg tttgtcagtg ttctaataat
901 agcaaaaaat aaaaaacaaa tgattgaaaa ataaaaaaaa aaaaaaaaa
//