LOCUS BC067295 2098 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens polymerase (RNA) II (DNA directed) polypeptide A,
220kDa, mRNA (cDNA clone IMAGE:30378439), complete cds.
ACCESSION BC067295
VERSION BC067295.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2098)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2098)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein
cDNA Library Preparation: Invitrogen Corp
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: m Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14589948
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2098
/db_xref="H-InvDB:HIT000262540"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30378439"
/tissue_type="Testis"
/clone_lib="NIH_MGC_180"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene 1..2098
/gene="POLR2A"
/gene_synonym="hRPB220"
/gene_synonym="hsRPB1"
/gene_synonym="POLR2"
/gene_synonym="POLRA"
/gene_synonym="RPB1"
/gene_synonym="RPBh1"
/gene_synonym="RpIILS"
/gene_synonym="RPO2"
/gene_synonym="RPOL2"
/db_xref="GeneID:5430"
/db_xref="MIM:180660"
CDS 146..1846
/gene="POLR2A"
/gene_synonym="hRPB220"
/gene_synonym="hsRPB1"
/gene_synonym="POLR2"
/gene_synonym="POLRA"
/gene_synonym="RPB1"
/gene_synonym="RPBh1"
/gene_synonym="RpIILS"
/gene_synonym="RPO2"
/gene_synonym="RPOL2"
/codon_start=1
/product="POLR2A protein"
/protein_id="AAH67295.1"
/db_xref="GeneID:5430"
/db_xref="MIM:180660"
/translation="MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYP
ETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVK
TMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEM
DNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSP
ERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDL
THKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGR
PLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAE
IVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHM
CDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQS
LETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERVCGPNGNLA
"
BASE COUNT 498 a 548 c 617 g 435 t
ORIGIN
1 ccaccttccc ctcctccggc tttttcctcc caactcgggg aggtccttcc cggtggccgc
61 cctgacgagg tctgagcacc taggcggagg cggcgcaggc tttttgtagt gaggtttgcg
121 cctgcgcagc gcgcctgcct ccgccatgca cgggggtggc cccccctcgg gggacagcgc
181 atgcccgctg cgcaccatca agagagtcca gttcggagtc ctgagtccgg atgaactgaa
241 gcgaatgtct gtgacggagg gtggcatcaa atacccagag acgactgagg gaggccgccc
301 caagcttggg gggctgatgg acccgaggca gggggtgatt gagcggactg gccgctgcca
361 aacatgtgca ggaaacatga cagagtgtcc tggccacttt ggccacattg aactggccaa
421 gcctgtgttt cacgtgggct tcctggtgaa gacaatgaaa gttttgcgct gtgtctgctt
481 cttctgctcc aaactgcttg tggactctaa caacccaaag atcaaggata tcctggctaa
541 gtccaaggga cagcccaaga agcggctcac acatgtctac gacctttgca agggcaaaaa
601 catatgcgag ggtggggagg agatggacaa caagttcggt gtggaacaac ctgagggtga
661 cgaggatctg accaaagaaa agggccatgg tggctgtggg cggtaccagc ccaggatccg
721 gcgttctggc ctagagctgt atgcggaatg gaagcacgtt aatgaggact ctcaggagaa
781 gaagatcctg ctgagtccag agcgagtgca tgagatcttc aaacgcatct cagatgagga
841 gtgttttgtg ctgggcatgg agccccgcta tgcacggcca gagtggatga ttgtcacagt
901 gctgcctgtg cccccgctct ccgtgcggcc tgctgttgtg atgcagggct ctgcccgtaa
961 ccaggatgac ctgactcaca aactggctga catcgtgaag atcaacaatc agctgcggcg
1021 caatgagcag aacggcgcag cggcccatgt cattgcagag gatgtgaagc tcctccagtt
1081 ccatgtggcc accatggtgg acaacgagct gcctggcttg ccccgtgcca tgcagaagtc
1141 tgggcgtccc ctcaagtccc tgaagcagcg gttgaagggc aaggaaggcc gggtgcgagg
1201 gaacctgatg ggcaaaagag tggacttctc ggcccgtact gtcatcaccc ccgaccccaa
1261 cctctccatt gaccaggttg gcgtgccccg ctccattgct gccaacatga cctttgcgga
1321 gattgtcacc cccttcaaca ttgacagact tcaagaacta gtgcgcaggg ggaacagcca
1381 gtacccaggc gccaagtaca tcatccgaga caatggtgat cgcattgact tgcgtttcca
1441 ccccaagccc agtgaccttc acctgcagac cggctataag gtggaacggc acatgtgtga
1501 tggggacatt gttatcttca accggcagcc aactctgcac aaaatgtcca tgatggggca
1561 tcgggtccgc attctcccat ggtctacctt tcgcttgaat cttagcgtga caactccgta
1621 caatgcagac tttgacgggg atgagatgaa cttgcacctg ccacagtctc tggagacgcg
1681 agcagagatc caggagctgg ccatggttcc tcgcatgatt gtcacccccc agagcaatcg
1741 gcctgtcatg ggtattgtgc aggacacact cacagcagtg cgcaaattca ccaagagaga
1801 cgtcttcctg gagcgggtgt gtggtccaaa tggaaacctg gcttaagtgg gcagtggggc
1861 tctggggtgc aaggtggagg ctagagagga agagctgtgt tttttttcct gacttaccca
1921 gcagtggtct gtgagattgt cttttctggt gggcgaacaa aaagggggtt aggaaaactc
1981 aggccaaaaa ggtgtaaggc gttaattccc catttaattc cttaaaattt catgtaatac
2041 caggtattgc ctgtaaagga aagataaagg gaaaaataag taaaaaaaaa aaaaaaaa
//