LOCUS BC066224 2534 bp mRNA linear ROD 18-MAR-2009
DEFINITION Mus musculus left-right determination factor 2, mRNA (cDNA clone
MGC:76786 IMAGE:30008028), complete cds.
ACCESSION BC066224
VERSION BC066224.1
KEYWORDS MGC.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 2534)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2534)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-FEB-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Kenneth R. Boheler (National Insitute on
Aging, USA)
cDNA Library Preparation: Yulan Piao and Minoru Ko (National
Institute on Aging, NIH: http://lgsun.grc.nia.nih.gov/cDNA/)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 144 Row: a Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 42734479.
FEATURES Location/Qualifiers
source 1..2534
/organism="Mus musculus"
/mol_type="mRNA"
/strain="129/Sv x 129/Sv-CP"
/db_xref="taxon:10090"
/clone="MGC:76786 IMAGE:30008028"
/tissue_type="ES Cell, undifferentiated, mouse, R1
Embryonic stem cells"
/clone_lib="NIA Mouse Undifferentiated ES Cell cDNA
Library (Long)"
/lab_host="DH10B"
/note="Vector: pSPORT1"
gene 1..2534
/gene="Lefty2"
/gene_synonym="Ebaf"
/gene_synonym="Lefta"
/gene_synonym="Leftb"
/db_xref="GeneID:320202"
/db_xref="MGI:MGI:2443573"
CDS 66..1172
/gene="Lefty2"
/gene_synonym="Ebaf"
/gene_synonym="Lefta"
/gene_synonym="Leftb"
/codon_start=1
/product="left-right determination factor 2"
/protein_id="AAH66224.1"
/db_xref="GeneID:320202"
/db_xref="MGI:MGI:2443573"
/translation="MKSLWLCWALWVLPLAGPGAAMTEEQVLSSLLQQLQLSQAPTLD
SADVEEMAIPTHVRSQYVALLQGSHADRSRGKRFSQNFREVAGRFLMSETSTHLLVFG
MEQRLPPNSELVQAVLRLFQEPVPRTALRRFERLSPHSARARVTIEWLRVREDGSNRT
ALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSAHKL
VRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEVPVTEGTRCCRQEMYLDLQG
MKWAENWILEPPGFLTYECVGSCLQLPESLTIGWPFLGPRQCVASEMTSLPMIVSVKE
GGRTRPQVVSLPNMRVQTCSCASDGALIPRGIDL"
BASE COUNT 667 a 590 c 677 g 600 t
ORIGIN
1 gtcccaagaa cttttcaggg cacttttagg gacgcatata tccacgattc ctcctgggca
61 gcgccatgaa gtccctgtgg ctttgctggg cactctgggt actgcccctg gctggccctg
121 gggcagcgat gaccgaggaa caggtcctga gcagtctact gcagcagctg cagctcagcc
181 aggcccccac cctggacagc gcggatgtgg aggagatggc catccctacc cacgtgaggt
241 cccagtatgt ggccctgctg cagggaagtc acgctgaccg ctcccgaggc aagaggttca
301 gccagaattt tcgagaggtg gcaggcaggt tcctgatgtc agagacctcc actcacctgc
361 tagtgttcgg aatggagcag cggctgccgc ctaacagcga gctggtgcag gctgtgctgc
421 ggctgttcca ggagcctgtg cccagaacag ctctccggag gtttgagagg ctgtccccac
481 acagtgcccg ggctcgggtc accattgaat ggctgagagt ccgtgaggat ggctccaatc
541 gcactgccct catcgactct aggctcgtgt ccatccacga gagcggctgg aaggccttcg
601 acgtgaccga ggccgtgaac ttctggcagc agctgagccg gccgaggcag ccgctgctgc
661 tccaggtgtc ggtgcagagg gagcatctgg ggccggggac ctggagcgca cacaagttgg
721 tccgtttcgc ggcgcagggg acgccggacg gcaaggggca gggcgagcca cagctggagc
781 tgcacacgct ggacctcaag gactacggag ctcaaggcaa ttgtgacccc gaggtaccag
841 tgactgaagg cacccgatgc tgtcgccagg agatgtacct ggacctgcag gggatgaagt
901 gggccgagaa ctggatccta gaaccgccag ggttcctgac gtatgaatgt gtgggcagct
961 gcctgcagct accagagtcc ctgaccatcg ggtggccatt tctggggcct cggcagtgtg
1021 ttgcctcaga gatgacctcc ttgcccatga ttgtcagtgt gaaggaggga ggcaggacca
1081 ggcctcaagt ggtcagcctg cccaacatga gggtgcagac ctgtagctgc gcctcagatg
1141 gggcgctcat acccaggggg atagatctgt agtctccctg tccacagatg tattctcagt
1201 gagcttgtcc taacttagtg ctctcgtcag acctttgctc tacagtcttg gttttcttgt
1261 ccatcaccca gtttaagcac ttacatgggt aaatcatgtc actccagtag gacacactga
1321 ccccacttag ccaaggacat ggctatgcag tgaacaggtt cgcatctgag tctgttttct
1381 ggccagaact cagcttaatg tacaacaaaa ccctacggtg agaacagggg aatcaaaagc
1441 tcgtttactc ttacaccgtg attactggca tcaacgtacc atgtcaggga ctgcccacag
1501 caggctggga gggagacatc tcagaagcct gcggcagctc cttgtgaaaa accgttgttc
1561 ccatttctcc taaccttagc cctagacaag agctgtatag atttcatgtg tgtgactgct
1621 tttcagttgg ccttggtgtt catagttatt ctatattatt tgactttcct actcctttct
1681 ccttctgccc tggtgaattc tatgaaacta gatgttcctt gatgtaatga ttcttaaaca
1741 attaaaaagt tgaggcatgg gacacagcac agcacagtcc tgatggccca ggtgcatgct
1801 gtagatgtat tctgtgtgct cttatcttgg aaacaatgca ataactttgc aatgttagtt
1861 cagattaatg tttgacttgc aaagaaagtt tgaagaaatt attagaaagt gaaatagagc
1921 caacactggg atcccgaaaa gaaaaaagct attgaagtta tgaaataagt tttgcacaaa
1981 atttgagagt gtttcctgga taagcaagta tagaatacat aaaatcttat attagtaaaa
2041 ctaagccaaa acaccgggac tcttaggagg gtcactgcgt gcaatgtgca gaagcagaaa
2101 gctggcagaa ctgccgagtt aagggtgtac ctgagtcttt ctggccattg cctggcagct
2161 ttgcccatgt catttattgt cagagcttca cgggaaaatg caagtagccg acttcggagc
2221 tctgagctct ggagtataat aagtcaaaag gtaaagttta aataatgata agtttgcaat
2281 aattattatt ttggccagag gcctgggaat aggggaagct tgaaactctg ggggaacaat
2341 tataattctt gattctttgt gtgatgtggg tattgttttg aatttgattt ggcaacgatt
2401 atacaatgtc tttttttcct atctgcattt ggagtatcaa taaaagactg gggcaagaga
2461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2521 aaaaaaaaaa aaaa
//