LOCUS BC066116 2561 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens alkaline phosphatase, liver/bone/kidney, mRNA (cDNA
clone MGC:60186 IMAGE:5754297), complete cds.
ACCESSION BC066116
VERSION BC066116.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2561)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2561)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-FEB-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 110 Row: g Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13787192.
FEATURES Location/Qualifiers
source 1..2561
/db_xref="H-InvDB:HIT000262117"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:60186 IMAGE:5754297"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2561
/gene="ALPL"
/gene_synonym="AP-TNAP"
/gene_synonym="TNAP"
/gene_synonym="TNSALP"
/db_xref="GeneID:249"
/db_xref="HGNC:HGNC:438"
/db_xref="MIM:171760"
CDS 185..1759
/gene="ALPL"
/gene_synonym="AP-TNAP"
/gene_synonym="TNAP"
/gene_synonym="TNSALP"
/codon_start=1
/product="alkaline phosphatase, liver/bone/kidney"
/protein_id="AAH66116.2"
/db_xref="GeneID:249"
/db_xref="HGNC:HGNC:438"
/db_xref="MIM:171760"
/translation="MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQ
KLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYN
TNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKS
VGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVI
MGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELL
TLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGG
RIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRG
NSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLR
HETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAA
GPLLLALALYPLSVLF"
BASE COUNT 602 a 823 c 665 g 471 t
ORIGIN
1 cgccactccg cgcccgctat cctggctccg tgctcccacg cgcttgtgcc tggacggacc
61 ctcgccagtg ctctgcgcag gattggaaca tcagttaaca tctgaccact gccagcccac
121 cccctcccac ccacgtcgat tgcatctctg ggctccaggg ataaagcagg tcttggggtg
181 caccatgatt tcaccattct tagtactggc cattggcacc tgccttacta actccttagt
241 gccagagaaa gagaaagacc ccaagtactg gcgagaccaa gcgcaagaga cactgaaata
301 tgccctggag cttcagaagc tcaacaccaa cgtggctaag aatgtcatca tgttcctggg
361 agatgggatg ggtgtctcca cagtgacggc tgcccgcatc ctcaagggtc agctccacca
421 caaccctggg gaggagacca ggctggagat ggacaagttc cccttcgtgg ccctctccaa
481 gacgtacaac accaatgccc aggtccctga cagcgccggc accgccaccg cctacctgtg
541 tggggtgaag gccaatgagg gcaccgtggg ggtaagcgca gccactgagc gttcccggtg
601 caacaccacc caggggaacg aggtcacctc catcctgcgc tgggccaagg acgctgggaa
661 atctgtgggc attgtgacca ccacgagagt gaaccatgcc acccccagcg ccgcctacgc
721 ccactcggct gaccgggact ggtactcaga caacgagatg ccccctgagg ccttgagcca
781 gggctgtaag gacatcgcct accagctcat gcataacatc agggacattg acgtgatcat
841 ggggggtggc cggaaataca tgtaccccaa gaataaaact gatgtggagt atgagagtga
901 cgagaaagcc aggggcacga ggctggacgg cctggacctc gttgacacct ggaagagctt
961 caaaccgaga tacaagcact cccacttcat ctggaaccgc acggaactcc tgacccttga
1021 cccccacaat gtggactacc tattgggtct cttcgagcca ggggacatgc agtacgagct
1081 gaacaggaac aacgtgacgg acccgtcact ctccgagatg gtggtggtgg ccatccagat
1141 cctgcggaag aaccccaaag gcttcttctt gctggtggaa ggaggcagaa ttgaccacgg
1201 gcaccatgaa ggaaaagcca agcaggccct gcatgaggcg gtggagatgg accgggccat
1261 cgggcaggca ggcagcttga cctcctcgga agacactctg accgtggtca ctgcggacca
1321 ttcccacgtc ttcacatttg gtggatacac cccccgtggc aactctatct ttggtctggc
1381 ccccatgctg agtgacacag acaagaagcc cttcactgcc atcctgtatg gcaatgggcc
1441 tggctacaag gtggtgggcg gtgaacgaga gaatgtctcc atggtggact atgctcacaa
1501 caactaccag gcgcagtctg ctgtgcccct gcgccacgag acccacggcg gggaggacgt
1561 ggccgtcttc tccaagggcc ccatggcgca cctgctgcac ggcgtccacg agcagaacta
1621 cgtcccccac gtgatggcgt atgcagcctg catcggggcc aacctcggcc actgtgctcc
1681 tgccagctcg gcaggcagcc ttgctgcagg ccccctgctg ctcgcgctgg ccctctaccc
1741 cctgagcgtc ctgttctgag ggcccggggc ccgggcaccc acaagcccgt gacagatgcc
1801 aacttcccac acggcagccc ccccctcaag gggcagggag gtgggggcct cctcagcctc
1861 tgcaactgca agaaagggga cccaagaaac caaagtctgc cgcccacctc gctcccctct
1921 ggaatcttcc ccaagggcca aacccacttc tggcctccag cctttgctcc ctccccgctg
1981 ccctttggcc aacagggtag atttctcttg ggcaggcaga gagtacagac tgcagacatt
2041 ctcaaagcct cttatttttc tagcgaacgt atttctccag acccagaggc cctgaagcct
2101 ccgtggaaca ttctggatct gaccctccca gtctcatctc ctgaccttcc cactcccatc
2161 tccttacctc tggaaccccc caggccctac aatgctcatg tccctgtccc caggcccagc
2221 cctccttcag gggagttgag gtctttctcc tcaggacaag gccttgctca ctcactcact
2281 ccaagaccac cagggtccca ggaagccggt gcctgggtgg ccatcctacc cagcgtggcc
2341 caggccggga agagccacct ggcagggctc acactcctgg gctctgaaca cacacgccag
2401 ctcctctctg aagcgactct cctgtttgga acggcaaaaa aaaatttttt tttctctttt
2461 tggtggtggt taaaagggaa cacaaaacat ttaaataaaa ctttccaaat attaaaaaaa
2521 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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