LOCUS BC065937 3551 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens 5'-nucleotidase, ecto (CD73), mRNA (cDNA clone
MGC:75146 IMAGE:6163101), complete cds.
ACCESSION BC065937
VERSION BC065937.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3551)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3551)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: n Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505466.
FEATURES Location/Qualifiers
source 1..3551
/db_xref="H-InvDB:HIT000262115"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:75146 IMAGE:6163101"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3551
/gene="NT5E"
/gene_synonym="CD73"
/gene_synonym="E5NT"
/gene_synonym="eN"
/gene_synonym="eNT"
/gene_synonym="NT"
/gene_synonym="NTE"
/db_xref="GeneID:4907"
/db_xref="HGNC:HGNC:8021"
/db_xref="MIM:129190"
CDS 26..1750
/gene="NT5E"
/gene_synonym="CD73"
/gene_synonym="E5NT"
/gene_synonym="eN"
/gene_synonym="eNT"
/gene_synonym="NT"
/gene_synonym="NTE"
/codon_start=1
/product="5'-nucleotidase, ecto (CD73)"
/protein_id="AAH65937.1"
/db_xref="GeneID:4907"
/db_xref="HGNC:HGNC:8021"
/db_xref="MIM:129190"
/translation="MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQT
SEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVA
HFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLP
YKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIAL
GHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVP
VVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNY
STQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADETFWNHVSMCILNGG
GIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFL
QVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGF
QMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSTGSHCHGSFSLIFLSLW
AVIFVLYQ"
BASE COUNT 1097 a 740 c 748 g 966 t
ORIGIN
1 cgcacccagt tcacgcgcca cagctatgtg tccccgagcc gcgcgggcgc ccgcgacgct
61 actcctcgcc ctgggcgcgg tgctgtggcc tgcggctggc gcctgggagc ttacgatttt
121 gcacaccaac gacgtgcaca gccggctgga gcagaccagc gaggactcca gcaagtgcgt
181 caacgccagc cgctgcatgg gtggcgtggc tcggctcttc accaaggttc agcagatccg
241 ccgcgccgaa cccaacgtgc tgctgctgga cgccggcgac cagtaccagg gcactatctg
301 gttcaccgtg tacaagggcg ccgaggtggc gcacttcatg aacgccctgc gctacgatgc
361 catggcactg ggaaatcatg aatttgataa tggtgtggaa ggactgatcg agccactcct
421 caaagaggcc aaatttccaa ttctgagtgc aaacattaaa gcaaaggggc cactagcatc
481 tcaaatatca ggactttatt tgccatataa agttcttcct gttggtgatg aagttgtggg
541 aatcgttgga tacacttcca aagaaacccc ttttctctca aatccaggga caaatttagt
601 gtttgaagat gaaatcactg cattacaacc tgaagtagat aagttaaaaa ctctaaatgt
661 gaacaaaatt attgcactgg gacattcggg ttttgaaatg gataaactca tcgctcagaa
721 agtgaggggt gtggacgtcg tggtgggagg acactccaac acatttcttt acacaggcaa
781 tccaccttcc aaagaggtgc ctgctgggaa gtacccattc atagtcactt ctgatgatgg
841 gcggaaggtt cctgtagtcc aggcctatgc ttttggcaaa tacctaggct atctgaagat
901 cgagtttgat gaaagaggaa acgtcatctc ttcccatgga aatcccattc ttctaaacag
961 cagcattcct gaagatccaa gcataaaagc agacattaac aaatggagga taaaattgga
1021 taattattct acccaggaat tagggaaaac aattgtctat ctggatggct cctctcaatc
1081 atgccgcttt agagaatgca acatgggcaa cctgatttgt gatgcaatga ttaacaacaa
1141 cctgagacac gcggatgaaa cgttctggaa ccacgtatcc atgtgcattt taaatggagg
1201 tggtatccgg tcgcccattg atgaacgcaa caatggcaca attacctggg agaacctggc
1261 tgctgtattg ccctttggag gcacatttga cctagtccag ttaaaaggtt ccaccctgaa
1321 gaaggccttt gagcatagcg tgcaccgcta cggccagtcc actggagagt tcctgcaggt
1381 gggcggaatc catgtggtgt atgatctttc ccgaaaacct ggagacagag tagtcaaatt
1441 agatgttctt tgcaccaagt gtcgagtgcc cagttatgac cctctcaaaa tggacgaggt
1501 atataaggtg atcctcccaa acttcctggc caatggtgga gatgggttcc agatgataaa
1561 agatgaatta ttaagacatg actctggtga ccaagatatc aacgtggttt ctacatatat
1621 ctccaaaatg aaagtaattt atccagcagt tgaaggtcgg atcaagtttt ccacaggaag
1681 tcactgccat ggaagctttt ctttaatatt tctttcactt tgggcagtga tctttgtttt
1741 ataccaatag ccaaaaattc tccttgcctt taatgtgtga aactgcattt tttcaagtga
1801 gattcaaatc tgccttttag gacctggctt tgtgacagca aaaaccatct ttacaggctc
1861 ctagaagctg aaggttagag cattataaaa tgaagagaca gacatgatta ctcagggtca
1921 gcaacctagt gagttagaaa aaaaattaac atagggccct ataaggagaa agccaactat
1981 gttaagttta tgtgtccaaa ttttaatgaa attttactaa caattttaaa ccatattttt
2041 cttcttcata tccatttcta atccatcaaa cagcttatgt ttacataaaa ttttatcatt
2101 cacaaggaag ttttaagcac actgtctcat ttgatatcca caacttattt ttggtaggaa
2161 agagagatgt ttttcccacc tgtcagatga aaaaactgaa gctcaaaaag ggttgacttg
2221 accatacagc taatgctgac agatccaaga cctagaccta ggtcttttga actcaagtcc
2281 aggattctca actatatcaa gttactgttc agaatactta atatctcctc tcttcataat
2341 tatcaatagc cccaagctca tggatgacaa atctctgctt tatttcttgt ctctattttt
2401 tcactttata gctcctgtta taatagcaag tttaatggta taaacacagg ataccatcct
2461 ctcttgcaac acccatgtgc ctttgatgag tcaggtagca agctgtagta gataatgaga
2521 aaggccagag gctgcaaaag acagtcaaag gacacgagag aaaggaaggg gaagaacagg
2581 actccaggac tgttttatat tatagaaaag caagagctaa agagcattta cacatgttaa
2641 acagatactt gttaagcata gtgcctgaca cacggcatta gctgttatga gattccatca
2701 gctctgcctc tgtcctcttt cttctaacat gaaggtatca tgagaagaga accttctaac
2761 ataagctgta attctaaacc tgcacttgtc cctctccagc aagaggctag cactgaattc
2821 attctactca tactacacac ccagttatgg aatgtccaga gttctcgaag aaaataaatg
2881 actttaggaa gaggtataca ttttttaagt cgctctgcct ccaaatctga acagtcactg
2941 taaatcattc ttaagcccag atatgagaac ttctgctgga aagtgggacc ctctgagtgg
3001 gtggtcagaa aatacccatg ctgatgaaat gacctatgcc caaagaacaa atacttaacg
3061 tgggagtgga accacatgag cctgctcagc tctgcataag taattcaaga aatgggaggc
3121 ttcaccttaa aaacagtgtg caaatggcag ctagaggttt tgataggaag tatgtttgtt
3181 tcttagtgtt tacaaatatt aagtactctt gatacaaaat atacttttaa acttcataac
3241 ctttttataa aagttgttgc agcaaaataa tagcctcggt tctatgcata tatggattag
3301 ctataaaaaa tgtcaataag attgtacaag gaaaattaga gaaaggcaca tttagggttt
3361 attttttaca cttggccagt aaaatagggt aaatcctatt agaatttttt aaagaacttt
3421 ttttaagttt cctaaatctg tgtgtgtatt gtgaagtggt ataagaaatg actttgaacc
3481 actttgcaat tgtagattcc caacaataaa attgaagata aaaaaaaaaa aaaaaaaaaa
3541 aaaaaaaaaa a
//