LOCUS BC065837 1080 bp mRNA linear HUM 30-JAN-2008
DEFINITION Homo sapiens chromosome 8 open reading frame 74, mRNA (cDNA clone
IMAGE:30383595).
ACCESSION BC065837
VERSION BC065837.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1080)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1080)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein
cDNA Library Preparation: Invitrogen Corp
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 141 Row: n Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: GenomeScan gene
prediction, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1080
/db_xref="H-InvDB:HIT000262077"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30383595"
/tissue_type="Testis"
/clone_lib="NIH_MGC_180"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6.1"
gene <1..1080
/gene="C8orf74"
/db_xref="GeneID:203076"
/db_xref="HGNC:HGNC:32296"
CDS <1..914
/gene="C8orf74"
/codon_start=3
/product="chromosome 8 open reading frame 74"
/protein_id="AAH65837.1"
/db_xref="GeneID:203076"
/db_xref="HGNC:HGNC:32296"
/translation="VSWQPDAGAMALLTPQGVKEVFQFQRPQGRERLRRLLNWEEFDE
QRDSRRSILLDTLYESIIFAVGKGFPWVEVAQVVKFTEELLRETKGCSITEAVTILGN
KLRDYRGHFNTTHLLALCDYFHHTFIRHYKLYQYVLGQDQQVDLTVAHLEVCMPPHPL
PLAEGMDRDLWIHEQQVATLTEAEAQKRADVLLLKEALRLERENSLQKAFAAAAPAQP
GQVLERQELESLICQAVHTQMELLQELLQRQIQNTFAILDLKLQKKTLNLNAPTPIPP
PITSHAGQEEALKPQRASKGKKAKARK"
BASE COUNT 301 a 325 c 295 g 159 t
ORIGIN
1 ccgtctcctg gcaaccagat gcaggggcca tggcactctt aacaccccag ggagtgaaag
61 aagtcttcca atttcagaga ccacaaggtc gggagcgcct gcggaggctt ctgaactggg
121 aggagtttga cgaacagaga gactcccgga ggagcatcct gctggacacc ctctacgaga
181 gcatcatctt tgcagtgggc aaaggcttcc catgggtgga ggtggcccag gtggtcaagt
241 tcacagaaga gctgctaagg gaaaccaaag gctgctccat tactgaggct gtgacgatcc
301 tggggaacaa gcttagagat taccggggcc atttcaacac cacccacctg ctggccctct
361 gtgactactt ccaccacacc ttcatccgcc actacaaact ctaccagtat gtcctgggcc
421 aggaccagca ggtcgacctg accgttgccc acctggaggt gtgcatgcca ccccatcccc
481 tcccgctggc cgagggcatg gacagggact tgtggatcca cgagcagcag gtggccacac
541 tgacggaggc cgaggcacag aagcgcgccg acgtgctgct cctgaaagag gcgctgcgcc
601 tggagcggga gaactcgctg cagaaggcgt tcgctgccgc cgcgcctgcg cagcccggcc
661 aggtcctgga gagacaggag ttggagagcc tcatctgcca ggcagtccac acccagatgg
721 agctcctgca ggagctgctg cagcgccaga tccagaacac attcgccatc ttggacctga
781 agcttcagaa gaagactctg aacctcaacg cccccacccc tatcccgccc cccatcacca
841 gccacgcagg ccaggaggaa gccctgaagc cccaaagagc gagcaaagga aagaaagcga
901 aggcaaggaa gtagaaggtc ccgactgcca cacgagactg actggggacc agccacccat
961 aaccatgagc cttgcggcac ggtgagctca gcacccacag agagacttct tgtgattaaa
1021 agaaacaaac ccatgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//