LOCUS BC065560 2099 bp mRNA linear HUM 27-OCT-2006
DEFINITION Homo sapiens NFS1 nitrogen fixation 1 homolog (S. cerevisiae), mRNA
(cDNA clone MGC:74467 IMAGE:6101221), complete cds.
ACCESSION BC065560
VERSION BC065560.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2099)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2099)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: h Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32307129.
FEATURES Location/Qualifiers
source 1..2099
/db_xref="H-InvDB:HIT000261966"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74467 IMAGE:6101221"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_70"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2099
/gene="NFS1"
/gene_synonym="HUSSY-08"
/gene_synonym="IscS"
/gene_synonym="NIFS"
/db_xref="GeneID:9054"
/db_xref="HGNC:HGNC:15910"
/db_xref="MIM:603485"
CDS 50..1423
/gene="NFS1"
/gene_synonym="HUSSY-08"
/gene_synonym="IscS"
/gene_synonym="NIFS"
/codon_start=1
/product="NFS1 nitrogen fixation 1 homolog (S.
cerevisiae)"
/protein_id="AAH65560.1"
/db_xref="GeneID:9054"
/db_xref="HGNC:HGNC:15910"
/db_xref="MIM:603485"
/translation="MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPA
DTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAA
MERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVL
DSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEI
GRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRV
RVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIM
KSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVL
RAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLK
SIKWTQH"
BASE COUNT 510 a 517 c 556 g 516 t
ORIGIN
1 gggcccagga ctgcagcggc ttcggaaggt gggctctgcc agcgggacca tgctgctccg
61 agccgcttgg aggcgggcgg cagtggcggt gacagcggct ccagggccga agcccgcggc
121 gcccactcgg gggctgcgcc tgcgcgttgg agaccgtgct cctcagtctg cggttcccgc
181 agatacagcc gctgccccgg aggtggggcc agtgctgcga cctctctata tggatgtgca
241 agctacaact cctctggacc cccgggtgct tgatgccatg ctcccttacc taattaacta
301 ctatgggaac ccacactccc ggacacatgc ttatggctgg gagagtgagg cagccatgga
361 acgtgctcgt cagcaagtag catctctgat tggagctgat cctcgtgaga tcatttttac
421 tagtggtgct actgaatcca acaacatagc aattaagggg gtggcccgat tctacaggtc
481 acggaaaaag cacttgatca ccacccagac agaacacaaa tgtgtcttgg actcctgccg
541 ttcactggaa gctgagggct ttcaggtcac ctacctccca gtgcagaaga gtgggatcat
601 tgacctaaag gaactagagg ctgctatcca gccagatact agcctggtgt cagtcatgac
661 tgtgaacaat gagattggag tgaagcagcc tattgcagaa atagggcgga tttgcagttc
721 cagaaaggta tatttccata ctgatgcagc ccaggctgtt ggaaaaatcc cacttgatgt
781 caatgacatg aaaattgatc tcatgagcat tagtggtcac aaaatctacg gtcccaaagg
841 ggttggtgcc atctacatcc gtcgccggcc ccgtgtgcgt gtggaggccc tgcagagtgg
901 aggggggcag gagcggggta tgcggtctgg gacagtgccc acacccttag tggtggggct
961 gggggctgcg tgtgaggtgg cacagcaaga gatggagtat gaccacaagc gaatctcaaa
1021 gttgtcagag cggctgatac agaatataat gaagagcctt ccagatgtgg tgatgaatgg
1081 ggaccctaag caccattatc ccggctgtat caacctctcc tttgcatatg tggaagggga
1141 aagtctgctg atggcactga aggacgttgc cttatcctca gggagtgcct gcacctctgc
1201 atccctggag ccctcttatg tgcttagagc aattggcact gatgaggatt tagcgcactc
1261 ttctatcagg tttggaattg gccgcttcac tacagaggag gaagtggact acacagtgga
1321 gaaatgcatt cagcatgtga agcgtcttcg agaaatgagc cctctctggg agatggttca
1381 ggatggcatt gacctcaaga gcatcaagtg gacccaacac tagaagaata gggccctgac
1441 tttgtgctgg tctggcccct cctgcctcac caacccgtgc acaaccagac accttgttac
1501 acctagtgga tgctctagat tggtatagac cagttgactt cagcatcagt ccacctctat
1561 gacagaaaca caagaaaact gtctttccct agcttcagtt ccttgggtgt ggagcactcc
1621 ccatttcttc tcgggtctta aagtgtgtgg acattttcat cccgaagcca tagagacatt
1681 tgctgtcata ttgctgctgg gcacatctgt gctcttggtg aggagagcaa gaggaaccag
1741 aagaagtctc tttggtcagg gaccatgatg ctctacatgg acatttgagt cttcgtcttc
1801 tgctgctgct cggctggacc agcttcttta acagcaagca taatccactt caatgtaata
1861 ttttctgtag ctccaaaggc tatctcttca tattgactgc agacagactg aatggacagt
1921 ttcttagagg gcttgtctcc tttctaccct tgtcctcttt cctttccttt gacctaatgg
1981 agctagaaat atgtctgtga ctccaccagt tattctaata atttgttttc ttgaaaattg
2041 ttaatttcaa gactggagaa ataaactcac cttctattta aaaaaaaaaa aaaaaaaaa
//