LOCUS BC065552 4107 bp mRNA linear HUM 06-MAR-2007
DEFINITION Homo sapiens isoleucyl-tRNA synthetase, mRNA (cDNA clone MGC:70727
IMAGE:6043344), complete cds.
ACCESSION BC065552
VERSION BC065552.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4107)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4107)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: d Column: 20.
FEATURES Location/Qualifiers
source 1..4107
/db_xref="H-InvDB:HIT000261958"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70727 IMAGE:6043344"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4107
/gene="IARS"
/gene_synonym="IARS1"
/gene_synonym="ILRS"
/gene_synonym="PRO0785"
/db_xref="GeneID:3376"
/db_xref="HGNC:HGNC:5330"
/db_xref="MIM:600709"
CDS 461..3628
/gene="IARS"
/gene_synonym="IARS1"
/gene_synonym="ILRS"
/gene_synonym="PRO0785"
/codon_start=1
/product="IARS protein"
/protein_id="AAH65552.1"
/db_xref="GeneID:3376"
/db_xref="HGNC:HGNC:5330"
/db_xref="MIM:600709"
/translation="MESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYK
DVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLI
LMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKE
EEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDA
DKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNND
LCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEE
LSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKRE
FEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRK
KNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFL
IQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVP
RLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTEL
MYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRD
RKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAE
PDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVAEGHELHDEDIRLMYTFDQ
ATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITV
YYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITL
TRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGSAPSLINSSSTLLCQYINLQLL
NAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEI
TEDIPVKTLNMKTVYVSVLPTTADF"
BASE COUNT 1215 a 849 c 929 g 1114 t
ORIGIN
1 aacaaaatgc ttcaacaagt tccagaaaac ataaattttc ctgctgaaga agagaaaatc
61 ttggagtttt ggactgaatt taattgtttt caggaatgct taaagcaatc aaaacataaa
121 ccaaaattta ccttctatga tggtcctcct tttgcaactg gactgcctca ctatggacat
181 atacttgcgg gtacaattaa agatatagtt acaagatatg ctcaccagag tgggtttcat
241 gttgacagaa gatttggatg ggattgccat ggcttacctg tggaatatga aattgataag
301 acactgggaa tcagaggacc agaggatgtg gccaaaatgg ggattacaga gtataacaat
361 cagtgccgag caattgtgat gagatattct gctgagtgga agcagacttg gccgatggat
421 tgactttgac aatgactata aaactctgta tccacaattc atggaatcag tctggtgggt
481 cttcaaacaa ctctatgata aaggccttgt ttatagaggt gtgaaagtca tgcccttctc
541 tacggcatgt aacactccac tttccaactt cgagtcacac cagaattata aggatgttca
601 agatccttca gtatttgtaa ctttcccttt ggaagaagat gaaactgtat ctttagttgc
661 ttggacaacc actccctgga ctctacctag taaccttgct gtgtgtgtta atccagaaat
721 gcaatatgtg aaaattaaag atgttgccag aggacgatta ctcattttaa tggaagccag
781 attgtcagcc ctctataaat tggagagtga ctatgagatc cttgaaagat ttcctggtgc
841 ctatcttaaa ggcaagaagt acaggcccct gtttgactat ttcctgaagt gtaaagagaa
901 tggcgctttc actgtgcttg ttgacaacta tgtgaaggaa gaagaaggca caggggttgt
961 ccaccaagct ccttacttcg gtgctgagga ctatcgggtc tgtatggact ttaacattat
1021 tcggaaagac tcactccctg tttgccctgt ggatgcttca ggctgcttca caacggaggt
1081 gacagatttc gcaggacagt atgtgaagga tgctgacaaa agtatcatca ggactttgaa
1141 ggaacaaggc cgacttctgg ttgccaccac cttcactcac agctaccctt tttgctggag
1201 atcagacact cctctaattt acaaagcagt gcccagctgg tttgtgcgag tggagaacat
1261 ggtggaccag ctcctaagga acaatgacct gtgctactgg gtcccagagt tggtacgaga
1321 aaaacgattt ggaaattggc tgaaagatgc acgtgactgg acaatttcca gaaacagata
1381 ctggggcacc cccatcccac tgtgggtcag cgatgacttt gaggaggtgg tatgcattgg
1441 gtcagtggcg gaacttgaag aactgtcagg agcaaagatc tcagatctcc acagagagag
1501 tgttgaccac ctgaccattc cttcacgctg tgggaaggga tccttgcacc gcatctctga
1561 agtgtttgac tgttggtttg agagtggcag catgccctat gctcaggttc attacccgtt
1621 tgaaaacaag agggagtttg aggatgcttt tcctgcagat ttcattgccg agggcatcga
1681 ccaaaccaga ggatggtttt ataccctgct ggtgctggcc acggccctct ttggacaacc
1741 gcctttcaag aacgtaattg tgaatgggct tgtcctggca agtgatggcc aaaaaatgag
1801 caaacggaaa aagaattatc cagatccagt ttccatcatc cagaagtatg gtgctgatgc
1861 cctcagatta tatctgatta actcccctgt ggtgagagca gaaaacctcc gctttaaaga
1921 agagggtgtg cgggacgtcc ttaaggatgt actgctccca tggtacaatg cctatcgctt
1981 cttaatccag aacgttctga ggctccagaa ggaggaagaa atagaatttc tctacaatga
2041 gaacacggtt agagaaagcc ccaacattac agaccggtgg atcctgtcct tcatgcagtc
2101 tctcattggc ttctttgaga ctgaaatggc agcttatagg ctttatactg tggtgcctcg
2161 cctggtcaag tttgtagata ttctgaccaa ttggtatgtt agaatgaacc gcagaagatt
2221 aaagggtgaa aatgggatgg aggattgtgt catggcccta gaaaccttgt ttagtgttct
2281 gctttctctt tgcagactta tggctcccta cacacctttt ctcactgaat tgatgtacca
2341 gaatctaaag gtgctgattg accctgtttc tgttcaggac aaggacacac tcagcattca
2401 ctacctcatg ctgccccgtg ttcgagaaga attgattgac aagaaaacag agagtgcagt
2461 atctcagatg cagtctgtga ttgaacttgg aagagtgatc agagaccgaa aaactattcc
2521 cataaagtat cctttgaaag aaattgtggt tatccatcaa gatccagaag ctcttaaaga
2581 tatcaagtct ttggagaagt atatcattga ggaactcaat gttcgaaaag ttacactgtc
2641 tacagataaa aacaagtatg gcattcggct aagggcagaa ccagatcaca tggtcctggg
2701 gaagcgtctg aagggagcct ttaaggcagt gatgacgtcc atcaagcagt tgagcagtga
2761 ggagctggag cagttccaga agactgggac cattgttgcg gaaggccatg aattgcacga
2821 tgaagacatc cgcctcatgt acacctttga tcaggccaca ggtgggactg cgcaatttga
2881 agcacactca gatgctcagg ctttggtcct cttagatgtc actcctgacc agtcaatggt
2941 agatgaagga atggctcggg aagtcatcaa tcgcatacag aaacttcgca aaaagtgcaa
3001 tctggttcca actgatgaaa tcacagtgta ctataaagca aagtctgaag gaacatatct
3061 gaatagtgtt attgaaagcc acacagagtt catatttacc accataaagg ctcccttgaa
3121 accatatcca gtttctccat cggataaagt ccttattcaa gaaaaaacac agttgaaggg
3181 atctgaactg gaaattacac tcaccagagg atcttccctt cctggtcctg cttgtgcata
3241 tgtcaatctt aacatttgtg caaatggcag tgaacaaggt ggagtattgc tcctggaaaa
3301 tccaaaagga tcggctccct ctctgatcaa cagttctagt actcttcttt gtcagtatat
3361 caacctacag ctcctgaatg caaagccaca agagtgttta atggggacag tgggcactct
3421 cctgcttgaa aacccacttg ggcagaatgg actcacccac caaggtcttc tgtatgaagc
3481 agccaaggtg tttggccttc ggagcaggaa gctaaagctg tttctgaatg agacccaaac
3541 gcaggaaatt acagaagaca tccccgtgaa gactttgaat atgaagactg tgtatgtttc
3601 tgtgttacca acaacagcag acttctagca tgtacttatc aatgttgttc ggtcagccct
3661 tccctaatta cacctatccc ctacacatac atgcacatag acacacacat gaacacactg
3721 aagatatttc cttcaggtgt gtgtaaaata tgctgcttgg attgaaattc aaatgggatt
3781 gattagtcaa gtaacttgag acctcacagt aatcttcaca cttaacctta gacacctatg
3841 cagtcatgtt gggagcaggt tacaatgtta cttcagccca cagtttattt ctatacttga
3901 gttcttaagt acagaagata gaagtgattt aaatggcata gtatatatat cattttctgg
3961 ccttttaaaa tttatttgag acctcttgat gaaatggaca tattatatat ttctgccacc
4021 tggattttcc tggataattt gatggaatat tttaagtttc agtaaatcag aacaataaac
4081 aaactcagat ataaaaaaaa aaaaaaa
//