LOCUS BC065522 2851 bp mRNA linear HUM 03-NOV-2006
DEFINITION Homo sapiens vascular endothelial growth factor A, mRNA (cDNA clone
IMAGE:6006890), partial cds.
ACCESSION BC065522
VERSION BC065522.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2851)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2851)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: a Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 71051577.
FEATURES Location/Qualifiers
source 1..2851
/db_xref="H-InvDB:HIT000261929"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6006890"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2851
/gene="VEGFA"
/gene_synonym="VEGF-A"
/gene_synonym="VPF"
/db_xref="GeneID:7422"
/db_xref="HGNC:HGNC:12680"
/db_xref="MIM:192240"
CDS <1..909
/gene="VEGFA"
/gene_synonym="VEGF-A"
/gene_synonym="VPF"
/codon_start=1
/product="VEGFA protein"
/protein_id="AAH65522.2"
/db_xref="GeneID:7422"
/db_xref="HGNC:HGNC:12680"
/db_xref="MIM:192240"
/translation="AAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVV
RAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCA
DSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLL
SWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQ
EYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMS
FLQHNKCECRPKKDRARQEKCDKPRR"
BASE COUNT 732 a 685 c 730 g 704 t
ORIGIN
1 gcggcggcga gccgcgggca ggggccggag cccgcgcccg gaggcggggt ggagggggtc
61 ggggctcgcg gcgtcgcact gaaacttttc gtccaacttc tgggctgttc tcgcttcgga
121 ggagccgtgg tccgcgcggg ggaagccgag ccgagcggag ccgcgagaag tgctagctcg
181 ggccgggagg agccgcagcc ggaggagggg gaggaggaag aagagaagga agaggagagg
241 gggccgcagt ggcgactcgg cgctcggaag ccgggctcat ggacgggtga ggcggcggtg
301 tgcgcagaca gtgctccagc cgcgcgcgct ccccaggccc tggcccgggc ctcgggccgg
361 ggaggaagag tagctcgccg aggcgccgag gagagcgggc cgccccacag cccgagccgg
421 agagggagcg cgagccgcgc cggccccggt cgggcctccg aaaccatgaa ctttctgctg
481 tcttgggtgc attggagcct tgccttgctg ctctacctcc accatgccaa gtggtcccag
541 gctgcaccca tggcagaagg aggagggcag aatcatcacg aagtggtgaa gttcatggat
601 gtctatcagc gcagctactg ccatccaatc gagaccctgg tggacatctt ccaggagtac
661 cctgatgaga tcgagtacat cttcaagcca tcctgtgtgc ccctgatgcg atgcgggggc
721 tgctgcaatg acgagggcct ggagtgtgtg cccactgagg agtccaacat caccatgcag
781 attatgcgga tcaaacctca ccaaggccag cacataggag agatgagctt cctacagcac
841 aacaaatgtg aatgcagacc aaagaaagat agagcaagac aagaaaaatg tgacaagccg
901 aggcggtgag ccgggcagga ggaaggagcc tccctcaggg tttcgggaac cagatctctc
961 accaggaaag actgatacag aacgatcgat acagaaacca cgctgccgcc accacaccat
1021 caccatcgac agaacagtcc ttaatccaga aacctgaaat gaaggaagag gagactctgc
1081 gcagagcact ttgggtccgg agggcgagac tccggcggaa gcattcccgg gcgggtgacc
1141 cagcacggtc cctcttggaa ttggattcgc cattttattt ttcttgctgc taaatcaccg
1201 agcccggaag attagagagt tttatttctg ggattcctgt agacacaccc acccacatac
1261 atacatttat atatatatat attatatata tataaaaata aatatctcta ttttatatat
1321 ataaaatata tatattcttt ttttaaatta acagtgctaa tgttattggt gtcttcactg
1381 gatgtatttg actgctgtgg acttgagttg ggaggggaat gttcccactc agatcctgac
1441 agggaagagg aggagatgag agactctggc atgatctttt ttttgtccca cttggtgggg
1501 ccagggtcct ctcccctgcc caggaatgtg caaggccagg gcatgggggc aaatatgacc
1561 cagttttggg aacaccgaca aacccagccc tggcgctgag cctctctacc ccaggtcaga
1621 cggacagaaa gacagatcac aggtacaggg atgaggacac cggctctgac caggagtttg
1681 gggagcttca ggacattgct gtgctttggg gattccctcc acatgctgca cgcgcatctc
1741 gcccccaggg gcactgcctg gaagattcag gagcctgggc ggccttcgct tactctcacc
1801 tgcttctgag ttgcccagga ggccactggc agatgtcccg gcgaagagaa gagacacatt
1861 gttggaagaa gcagcccatg acagctcccc ttcctgggac tcgccctcat cctcttcctg
1921 ctccccttcc tggggtgcag cctaaaagga cctatgtcct cacaccattg aaaccactag
1981 ttctgtcccc ccaggagacc tggttgtgtg tgtgtgagtg gttgaccttc ctccatcccc
2041 tggtccttcc cttcccttcc cgaggcacag agagacaggg caggatccac gtgcccattg
2101 tggaggcaga gaaaagagaa agtgttttat atacggtact tatttaatat ccctttttaa
2161 ttagaaatta aaacagttaa tttaattaaa gagtagggtt ttttttcagt attcttggtt
2221 aatatttaat ttcaactatt tatgagatgt atcttttgct ctctcttgct ctcttatttg
2281 taccggtttt tgtatataaa attcatgttt ccaatctctc tctccctgat cggtgacagt
2341 cactagctta tcttgaacag atatttaatt ttgctaacac tcagctctgc cctccccgat
2401 cccctggctc cccagcacac attcctttga aataaggttt caatatacat ctacatacta
2461 tatatatatt tggcaacttg tatttgtgtg tatatatata tatatatgtt tatgtatata
2521 tgtgattctg ataaaataga cattgctatt ctgtttttta tatgtaaaaa caaaacaaga
2581 aaaaatagag aattctacat actaaatctc tctccttttt taattttaat atttgttatc
2641 atttatttat tggtgctact gtttatccgt aataattgtg gggaaaagat attaacatca
2701 cgtctttgtc tctagtgcag tttttcgaga tattccgtag tacatattta tttttaaaca
2761 acgacaaaga aatacagata tatcttaaaa aaaaaaaagc attttgtatt aaagaattta
2821 attctgatct caaaaaaaaa aaaaaaaaaa a
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