LOCUS BC065384 5844 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens PCF11, cleavage and polyadenylation factor subunit,
homolog (S. cerevisiae), mRNA (cDNA clone MGC:65021 IMAGE:6043635),
complete cds.
ACCESSION BC065384
VERSION BC065384.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5844)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5844)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: k Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33620744.
FEATURES Location/Qualifiers
source 1..5844
/db_xref="H-InvDB:HIT000261885"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65021 IMAGE:6043635"
/tissue_type="Testis, embryonal carcinoma"
/clone_lib="NIH_MGC_92"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5844
/gene="PCF11"
/db_xref="GeneID:51585"
/db_xref="HGNC:HGNC:30097"
/db_xref="MIM:608876"
CDS 238..4905
/gene="PCF11"
/codon_start=1
/product="PCF11, cleavage and polyadenylation factor
subunit, homolog (S. cerevisiae)"
/protein_id="AAH65384.2"
/db_xref="GeneID:51585"
/db_xref="HGNC:HGNC:30097"
/db_xref="MIM:608876"
/translation="MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILA
EENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFIC
VFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIH
VNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLEL
QQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQS
EKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIP
SEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESM
RLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKII
NGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQR
SMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERP
QETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRES
WSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDF
LVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQ
RLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQM
GGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRF
EGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGG
LRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQ
HNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPR
FEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFD
GVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAP
SQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFG
NIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDN
HLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTN
FTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRK
VTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGP
AGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSP
SKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTV
ESV"
BASE COUNT 1909 a 1122 c 1331 g 1482 t
ORIGIN
1 gtggagaaag aagcttctgt ggcggctgga agtggacgga gatcacccgc gagacggcgg
61 cgtttcatac ccgaggttcc ccctgtgtcg tcccccatcc cccctccgcg gtcagcatgt
121 ggtgaagccg gagccgcgag agagccgggg agaggaagag gagtcggaag ggaggcgggg
181 tatccagagc ggcttcagct tcagctgcag cggacctcgg aggggggccg cggcgcaatg
241 tcagagcaga cgccggccga ggccggtgct gcgggggccc gggaggacgc ctgtcgggat
301 tatcagtcat cgctcgaaga cctgaccttc aatagcaagc cgcacatcaa tatgctgacc
361 attctagccg aggagaacct gcccttcgcc aaggagatcg tctctctcat cgaggcccaa
421 accgccaagg ctccttcctc agagaagctt cctgttatgt accttatgga ttctattgtg
481 aaaaacgttg gaagagagta tctcactgcc tttactaaaa atctagttgc aacatttatt
541 tgtgtgtttg aaaaggtgga tgaaaatact aggaaaagtt tatttaagtt acgttctaca
601 tgggatgaaa tattcccttt gaagaaactt tatgccctgg atgtcagagt caattcatta
661 gatcctgctt ggcctattaa acctctaccc cccaatgtga atacgtctag catccatgtg
721 aatcctaaat ttttaaataa atcgcccgag gagccttcaa cacctggtac agtggtcagt
781 tcccctagca tctccactcc tccaattgtt cctgatatac aaaagaatct tacacaagaa
841 caactaataa ggcagcagtt actggcaaag caaaaacagt tgttagaact tcagcagaaa
901 aagctggagc ttgagctaga gcaagctaag gcacagttgg cagtttctct tagtgttcag
961 caggaaacat ccaatttagg tcctggatct gcaccatcca aattacatgt ttcacagatt
1021 ccccctatgg cagttaaagc tcctcatcag gttcctgtgc aatctgagaa aagccgtcca
1081 ggaccatcct tacaaattca ggatttaaaa ggaactaacc gggatcctcg tctgaacagg
1141 ataagccaac attctcatgg aaaagatcag agtcacagga aagaatttct aatgaacaca
1201 ttaaaccagt ctgatactaa gacaagtaaa actataccct ctgaaaaact aaattcatcc
1261 aagcaagaaa aaagtaaatc aggtgaaaaa ataaccaaga aagaacttga ccaattagat
1321 tctaaatcga aatcgaaatc gaaatcaccc tcacctttga aaaacaaatt atctcacaca
1381 aaagacttga aaaatcaaga atcggaaagt atgaggttgt ctgatatgaa caagagagat
1441 ccaagattaa aaaaacatct tcaggataag accgatggca aagatgatga tgtgaaagag
1501 aagagaaaaa ctgcagaaaa aaaggataaa gatgagcaca tgaagtcatc cgaacacaga
1561 ctggctggaa gtagaaataa aatcataaat ggcattgtac aaaaacagga tacaataaca
1621 gaagagtcag aaaaacaggg gacaaaacca gggagatcga gtactagaaa gcgatcaaga
1681 tctcgatcac ccaagtctag gtcaccaatt atacattccc caaagagaag agataggcgg
1741 tcacccaaac gaaggcaaag aagtatgtct ccaacatcga cacctaaagc tggaaagatt
1801 cgccaatctg gagctaagca gtcacatatg gaagagttta caccaccttc tagggaagac
1861 agaaatgcta agagaagtac taaacaggat attcgggatc caaggcgaat gaaaaagact
1921 gaagaggagc gaccacaaga aactacaaat cagcattcta caaagtcagg cactgaacca
1981 aaggagaatg tagaaaactg gcaaagttcc aagtctgcca aaagatggaa atctggttgg
2041 gaagaaaata aaagcttaca acaggttgat gaacatagta aacctcctca tctgaggcat
2101 agggagagct ggtcaagcac taaaggaatt ttatcacctc gagccccaaa gcagcaacag
2161 catcgattaa gtgtagatgc caatcttcag attcctaaag agttaactct tgcaagcaaa
2221 agagaattac ttcaaaagac gagtgaacgt ttagcatctg gtgaaattac acaggatgac
2281 ttccttgttg ttgtgcatca aattcgacag ctatttcagt atcaagaagg tgtgcgagaa
2341 gagcagagat ctccattcaa tgatcgtttt ccacttaagc gacctcgata tgaagattca
2401 gataaaccat ttgtagatag tccagcatca agattcgccg gcctggatac aaatcagcga
2461 cttacagctt tagctgaaga cagaccgtta tttgatggac ctagtaggcc atcagtagca
2521 agagatggcc caacgaagat gatttttgaa ggacccaata aattaagccc tcgaattgat
2581 ggacctccca caccagcttc tcttcggttt gatgggtcac caggacaaat ggggggagga
2641 ggccctttga gatttgaggg gccacaaggt cagctaggag gtgggtgtcc tttgagattt
2701 gaaggtcctc caggaccagt ggggacacct ctgcggtttg agggcccaat tggtcaagca
2761 ggaggaggtg gttttcggtt tgaaggttcc cctggtctga ggtttgaggg atctccaggt
2821 ggtttgagat ttgagggacc aggaggccag cctgtgggtg gtctgaggtt tgagggacat
2881 cgtggtcaac ctgtgggtgg tctaaggttt gagggacctc atggtcagcc tgtgggtgga
2941 cttagatttg ataatccccg aggtcagcct gtaggtggac ttagatttga ggggggtcat
3001 ggtccatcag gggctgcgat taggtttgat ggacctcatg gtcagccagg aggtggaatc
3061 agatttgagg gccctttgct acagcaaggg gttggaatga ggtttgaggg cccccatggt
3121 cagtcagtag ctggtctgag atttgaggga caacataatc aacttggtgg gaaccttagg
3181 tttgagggtc cacatggtca gccaggggtt ggtatcaggt ttgaaggccc tttagtccaa
3241 caaggaggtg gaatgaggtt tgagggtcct tctgtaccag gaggtggcct gagaattgaa
3301 gggcctctgg gtcaaggtgg tccaagattt gaaggttgtc atgctttaag gtttgatggg
3361 cagccaggtc agccgtcact cttgccaaga tttgatggat tacatggtca gccaggtcct
3421 agatttgaaa ggactcctgg tcagccaggc cctcagaggt ttgatggacc acctggacag
3481 caggttcaac ccagatttga cggtgtacct caaagatttg atggtccaca acatcagcaa
3541 gcatcaaggt ttgatattcc tcttggtctt caaggcacaa gatttgacaa tcatccttca
3601 caaaggcttg aatcagtatc tttcaatcag actggtccat ataatgatcc acctggcaat
3661 gcttttaatg ccccatccca aggactacag ttccaaagac atgaacaaat atttgattca
3721 cctcaaggac caaattttaa tggaccacat ggccctggaa accagagttt ctctaatcca
3781 cttaacagag cttctggaca ctattttgat gaaaaaaatc ttcagagttc tcaatttgga
3841 aactttggca atatacctgc tccaatgaca gtaggaaata ttcaggcatc tcaacaggtt
3901 ctgagtggtg ttgctcagcc agtagctttt ggtcaaggac aacagttttt accagttcat
3961 ccacaaaatc ctggatttgt tcagaatcct tcaggagccc tccctaaggc atatcctgat
4021 aatcatctca gtcaggtgga tgtaaatgaa ttgttttcaa aattgctaaa aacaggaatt
4081 ctcaaattgt cccaaactga ttcagctaca acacaagtaa gtgaagtaac tgctcagcct
4141 ccccctgaag aggaggaaga tcaaaatgaa gatcaagatg ttccagatct tactaatttt
4201 acagttgaag aattgaaaca acgttatgac agtgttataa atcgactgta cactggtatt
4261 cagtgttact cttgtggaat gaggtttaca acatcacaga cagatgttta tgcagatcat
4321 ttggactggc attatcggca aaatagaact gaaaaggatg ttagccgaaa agtcactcat
4381 agacgttggt actacagttt aacagactgg atagaatttg aggagatagc tgatctggaa
4441 gaacgggcaa agagccagtt ttttgaaaag gtgcatgaag aagttgtgct caaaactcaa
4501 gaggctgcta aagaaaaaga gttccaaagt gtacctgctg gaccagctgg agcagttgag
4561 agttgtgaaa tctgtcaaga acaatttgaa caatactggg atgaagaaga ggaggaatgg
4621 catttgaaaa atgctattag agtagatgga aagatttatc atccatcatg ttatgaagat
4681 tatcaaaata catcttcatt tgattgtaca ccatctccca gcaagacacc agttgaaaac
4741 cccttgaata ttatgttgaa cattgtcaaa aacgaattgc aggaaccctg tgacagtccc
4801 aaagttaagg aagaacgaat tgatacacca ccagcttgta cagaggaaag catagcaaca
4861 ccctctgaaa ttaaaacaga aaatgacaca gtcgagtcag tttaaataaa atgagaaagg
4921 tatgtttttc ttttttaaaa aagctgctgt tggatctaga aggtgaagaa tttttttatg
4981 tatatataga catatctata taaattgtct ggctgaggca gggccttcag ctatcatttg
5041 gttaataaat acattttagt atttgcattt cctactgcct gcagagtttc aggtgcttgt
5101 tgtgtgaaag ttctgtagat gtgtgcaaat ttaacgaaat gaaattgtat gtgtaaaaat
5161 gtacgatttt tcactgtgca actgtaaatt ataaataaaa aatatttttg ctattcatgg
5221 agtgtaatat ttatgcacac catcaaatag tttctgtact ttttattggg taaaaatgga
5281 attgaacagc aacctcaaca taagattttt tttctagtag cctcccactg attaaagaag
5341 caagtttgag gtttcatcct tcaaaagggg gttccgagag agcaccgtag ggcttttctc
5401 aaatagaaaa gccagatttt gaaaaaattt taaagataaa ataggacata ttttgcagat
5461 atatatatat atatacacaa acacatctcc aggtatagag aaccatccag atgttcactt
5521 ttgaaaatat ctaatgatgc aaagttttat tcttgaactt ggacactgat gccatcaaac
5581 aattaacaaa tatatttaag tactaaaggt gatttttttt ttaaagactt tttcaaattg
5641 tcaaatgatt taatgcagat gaacatattt ctattttaag taacgggaat ctgtaagaat
5701 gtttgcttga gatatggtta acttttttct tttgttggtt ttgacttaga tggacaccat
5761 gagatgttaa tattcataca tgtaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5821 aaaaaaaaaa aaaaaaaaaa aaaa
//