LOCUS BC065294 2860 bp mRNA linear HUM 19-MAR-2007
DEFINITION Homo sapiens aminopeptidase puromycin sensitive, mRNA (cDNA clone
MGC:70648 IMAGE:6059589), complete cds.
ACCESSION BC065294
VERSION BC065294.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2860)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2860)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: f Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 15451906.
FEATURES Location/Qualifiers
source 1..2860
/db_xref="H-InvDB:HIT000261856"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70648 IMAGE:6059589"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2860
/gene="NPEPPS"
/gene_synonym="MP100"
/gene_synonym="PSA"
/db_xref="GeneID:9520"
/db_xref="HGNC:HGNC:7900"
/db_xref="MIM:606793"
CDS 45..2672
/gene="NPEPPS"
/gene_synonym="MP100"
/gene_synonym="PSA"
/codon_start=1
/product="aminopeptidase puromycin sensitive"
/protein_id="AAH65294.2"
/db_xref="GeneID:9520"
/db_xref="HGNC:HGNC:7900"
/db_xref="MIM:606793"
/translation="MPEKRPFERLPADVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQ
ATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKI
DFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISL
VVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKD
GVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMEN
WGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFA
SWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAI
SYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPIAAVM
NTWTKQMGFPLIYVEAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDP
NQAKLKILMDKPEMNVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSL
PPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSH
TDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEAR
RRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLG
ATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRY
QGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKR
DAESIHQYLLQRKASPPTV"
BASE COUNT 793 a 597 c 698 g 772 t
ORIGIN
1 gccgctcctc tcgccgccgc ctccacagcc tgggcctcgc cgcgatgccg gagaagaggc
61 ccttcgagcg gctgcctgcc gatgtctccc ccatcaacta cagcctttgc ctcaagcccg
121 acttgctgga cttcaccttc gagggcaagc tggaggccgc cgcccaggtg aggcaggcga
181 ctaatcagat tgtgatgaat tgtgctgata ttgatattat tacagcttca tatgcaccag
241 aaggagatga agaaatacat gctacaggat ttaactatca gaatgaagat gaaaaagtca
301 ccttgtcttt ccctagtact ctgcaaacag gtacgggaac cttaaagata gattttgttg
361 gagagctgaa tgacaaaatg aaaggtttct atagaagtaa atatactacc ccttctggag
421 aggtgcgcta tgctgctgta acacagtttg aggctactga tgcccgaagg gcttttcctt
481 gctgggatga gcctgctatc aaagcaactt ttgatatctc attggttgtt cctaaagaca
541 gagtagcttt atcaaacatg aatgtaattg accggaaacc ataccctgat gatgaaaatt
601 tagtggaagt gaagtttgcc cgcacacctg ttatgtctac atatctggtg gcatttgttg
661 tgggtgaata tgactttgta gaaacaaggt caaaagatgg tgtgtgtgtc cgtgtttaca
721 ctcctgttgg caaagcagag caaggaaaat ttgcgttaga ggttgctgct aaaaccttgc
781 ctttttataa ggactacttc aatgttcctt atcctctacc taaaattgat ctcattgcta
841 ttgcagactt tgcagctggt gccatggaga actggggcct tgttacttat agggagactg
901 cattgcttat tgatccaaaa aattcctgtt cttcatcccg ccagtgggtt gctctggttg
961 tgggacatga actcgcccat caatggtttg gaaatcttgt tactatggaa tggtggactc
1021 atctttggtt aaatgaaggt tttgcatcct ggattgaata tctgtgtgta gaccactgct
1081 tcccagagta tgatatttgg actcagtttg tttctgctga ttacacccgt gcccaggagc
1141 ttgacgcctt agataacagc catcctattg aagtcagtgt gggccatcca tctgaggttg
1201 atgagatatt tgatgctata tcatatagca aaggtgcatc tgtcatccga atgctgcatg
1261 actacattgg ggataaggac tttaagaaag gaatgaacat gtatttaacc aagttccaac
1321 aaaagaatgc tgccacagag gatctctggg aaagtttaga aaatgctagt ggtaaaccta
1381 tagcagctgt gatgaatacc tggaccaaac aaatgggatt tcccctcatt tatgtggaag
1441 ctgaacaggt agaagatgac agattattga ggttgtccca aaagaagttc tgtgctggtg
1501 ggtcatatgt tggtgaagat tgtccccagt ggatggtccc tatcacaatc tctactagtg
1561 aagaccccaa ccaggccaaa ctaaaaattc taatggacaa gccagagatg aatgtggttt
1621 tgaaaaatgt caaaccagac caatgggtga agttaaactt aggaacagtt gggttttatc
1681 ggacccagta cagctctgcc atgctggaaa gtttattacc aggcattcgt gacctttctc
1741 tgccccctgt ggatcgactt ggattacaga atgacctctt ctccttggct cgagctggaa
1801 tcattagcac tgtagaggtt ctaaaagtca tggaggcttt tgtgaatgag cccaattata
1861 ctgtatggag cgacctgagc tgtaacctgg ggattctctc aactctcttg tcccacacag
1921 acttctatga ggaaatccag gagtttgtga aagatgtctt ttcacctata ggggagagac
1981 tgggctggga ccccaaacct ggagaaggtc atctcgatgc actcctgagg ggcttggttc
2041 tgggaaaact aggaaaagca ggacataagg caacgttaga agaagcccgt cgtcggttta
2101 aggaccacgt ggaaggaaaa cagattctct ccgctgatct gaggagtcct gtctatctga
2161 ctgttttgaa gcatggtgat ggcactactt tagatattat gttaaaactt cataaacaag
2221 cagatatgca agaagagaaa aaccgaatcg aaagagtcct tggcgctact cttttgcctg
2281 acctgattca aaaagtcctc acgtttgcac tttcagaaga ggtacgtcca caggacactg
2341 tatcggtaat tggtggagta gctggaggca gcaagcatgg taggaaagct gcttggaaat
2401 tcataaagga caactgggaa gaactttata accgatacca gggaggattc ttaatatcca
2461 gactaataaa gctatcagtt gagggatttg cagttgataa aatggctgga gaggttaagg
2521 ctttcttcga gagtcaccca gctccttcag ctgagcgtac catccagcag tgttgtgaaa
2581 atattctgct gaatgctgcc tggctaaagc gagatgctga gagcatccac cagtacctcc
2641 ttcagcggaa ggcctcacca cccacagtgt gaatcctgag gtgccgccat tggcggttct
2701 gctgcttcgc tgcagggata aggtggagct accgaacagc tgattcatat gccaagaatt
2761 tggagtcttc tttcaaacca gtgggggttg gacaatgaat gtagttaact ggttcctgct
2821 cacactccag aattaaattc tattgaaaaa aaaaaaaaaa
//