LOCUS BC065245 2060 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens dihydroorotate dehydrogenase, mRNA (cDNA clone
MGC:70636 IMAGE:6064723), complete cds.
ACCESSION BC065245
VERSION BC065245.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2060)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2060)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: f Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 68509923.
FEATURES Location/Qualifiers
source 1..2060
/db_xref="H-InvDB:HIT000261808"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70636 IMAGE:6064723"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2060
/gene="DHODH"
/gene_synonym="DHOdehase"
/db_xref="GeneID:1723"
/db_xref="HGNC:HGNC:2867"
/db_xref="MIM:126064"
CDS 2..1189
/gene="DHODH"
/gene_synonym="DHOdehase"
/codon_start=1
/product="dihydroorotate dehydrogenase"
/protein_id="AAH65245.1"
/db_xref="GeneID:1723"
/db_xref="HGNC:HGNC:2867"
/db_xref="MIM:126064"
/translation="MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPT
LQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGE
AVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRL
RARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTA
GLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGI
DGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGG
VSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGAD
HRR"
BASE COUNT 503 a 512 c 591 g 454 t
ORIGIN
1 catggcgtgg agacacctga aaaagcgggc ccaggatgct gtgatcatcc tggggggagg
61 aggacttctc ttcgcctcct acctgatggc cacgggagat gagcgtttct atgctgaaca
121 cctgatgccg actctgcagg ggctgctgga cccggagtca gcccacagac tggctgttcg
181 cttcacctcc ctggggctcc ttccacgggc cagatttcaa gactctgaca tgctggaagt
241 gagagttctg ggccataaat tccgaaatcc agtaggaatt gctgcaggat ttgacaagca
301 tggggaagcc gtggacggac tttataagat gggctttggt tttgttgaga taggaagtgt
361 gactccaaaa cctcaggaag gaaaccctag acccagagtc ttccgcctcc ctgaggacca
421 agctgtcatt aacaggtatg gatttaacag tcacgggctt tcagtggtgg aacacaggtt
481 acgggccaga cagcagaagc aggccaagct cacagaagat ggactgcctc tgggggtcaa
541 cttggggaag aacaagacct cagtggacgc cgcggaggac tacgcagaag gggtgcgcgt
601 actgggcccc ctggccgact acctggtggt gaatgtgtcc agccccaaca ctgccgggct
661 gcggagcctt cagggaaagg ccgagctgcg ccgcctgctg accaaggtgc tgcaggagag
721 ggatggcttg cggagagtgc acaggccggc agtcctggtg aagatcgctc ctgacctcac
781 cagccaggat aaggaggaca ttgccagtgt ggtcaaagag ttgggcatcg atgggctgat
841 tgttacgaac accaccgtga gtcgccctgc gggcctccag ggtgccctgc gctctgaaac
901 aggagggctg agtgggaagc ccctccggga tttatcaact caaaccattc gggagatgta
961 tgcactcacc caaggccgag ttcccataat tggggttggt ggtgtgagca gcgggcagga
1021 cgcgctggag aagatccggg caggggcctc cctggtgcag ctgtacacgg ccctcacctt
1081 ctgggggcca cccgttgtgg gcaaagtcaa gcgggaactg gaggcccttc tgaaagagca
1141 gggctttggc ggagtcacag atgccattgg agcagatcat cggaggtgag gacagcgtct
1201 gacgggaagc ctgatctgga accttcccaa ggactcaggc aagcctttgt ggctggatca
1261 tgagaggagg gactccatct tgagccatgt cccccagcca tggcatggct gcactgtaaa
1321 cgccaatcgg ggggtcacca ggatcaaccg caggctttct tcagtccctt ggtcagacca
1381 taaactgcat ttttgattct ttgtggattc aaaccctagg atccatcagt cttgcaagga
1441 cattgaatat taggaggaaa aagtcatgga aaaaataaag ccatttagaa cctgggtttc
1501 aacgctagcc ctttctggtt tgccataggc cctgccaaga tactgcaggt ccatccaggc
1561 ctctgctatc tgcatctgca gtgggcttcc caggaacttg actgtctttc atttgatctt
1621 tatttttgtt tatttaatat tttaaacttt attttaaaaa tatttcaaac ataagggcgg
1681 ggtgtggtgg ctcatgcctg taatcccagc actttgggag gccgaggcgg gcggatcacc
1741 tgaggccagg agttggagac catccaggcc accatggtga aaccctgtct ctaccaaaaa
1801 tacaaaaaat tagccagata ttgtggcagg cacttttaat cccagctact cgggaggctg
1861 aggcaggaga attgcttgaa cctgggaggc ggaggttgca gtgagccaag attgcaccag
1921 tgcactccag cttgggcgac agatcaagac tctgtcacac acaaaacaaa cgaacaaatt
1981 ttcccgtttc tctctgtccc tttctctata acatggataa aatattcacg tcttatgtat
2041 ttattaaaaa aaaaaaaaaa
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