LOCUS BC064978 2538 bp mRNA linear HUM 27-JAN-2004
DEFINITION Homo sapiens protein phosphatase 2C, magnesium-dependent, catalytic
subunit, mRNA (cDNA clone IMAGE:6158636), partial cds.
ACCESSION BC064978
VERSION BC064978.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2538)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2538)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: m Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8923959.
FEATURES Location/Qualifiers
source 1..2538
/db_xref="H-InvDB:HIT000261685"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6158636"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2538
/gene="PPM2C"
/gene_synonym="PDH"
/gene_synonym="PDP"
/db_xref="GeneID:54704"
/db_xref="MIM:605993"
CDS <1..1823
/gene="PPM2C"
/gene_synonym="PDH"
/gene_synonym="PDP"
/codon_start=3
/product="PPM2C protein"
/protein_id="AAH64978.1"
/db_xref="GeneID:54704"
/db_xref="MIM:605993"
/translation="GQAGGEGSRSGSCTGLRGKSAERWRRCRPLLVGKNRLVSCRARR
MCVCPGPRRIGIPVRSSSLPLFSDAMPAPTQLFFPLIRNCELSRIYGTACYCHHKHLC
CSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRRYASTPQKFYLTPPQVNSILKA
NEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGC
ACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKL
YFNSLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVL
RVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELE
RLKLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEY
TKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTG
MHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNNEFGTVDH
ERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQE"
misc_feature 738..1592
/gene="PPM2C"
/gene_synonym="PDH"
/gene_synonym="PDP"
/note="PP2C; Region: Protein phosphatase 2C. Protein
phosphatase 2C is a Mn++ or Mg++ dependent protein
serine/threonine phosphatase"
/db_xref="CDD:pfam00481"
BASE COUNT 690 a 552 c 589 g 707 t
ORIGIN
1 gtgggcaggc cgggggtgag ggctcgcgct ccgggagctg cacggggctg cgtggaaaga
61 gcgccgagcg gtggcgtcgt tgtcgccccc tcctcgtcgg gaagaatcgt ttggtctcct
121 gccgtgcccg gagaatgtgt gtgtgtcccg ggcccagacg aattggaatc ccagtcagaa
181 gttccagcct gccactgttc tctgatgcca tgccagcacc aactcaactg ttttttcctc
241 tcatccgtaa ctgtgaactg agcaggatct atggcactgc atgttactgc caccacaaac
301 atctctgttg ttcctcatcg tacattcctc agagtcgact gagatacaca cctcatccag
361 catatgctac cttttgcagg ccaaaggaga actggtggca gtacacccaa ggaaggagat
421 atgcttccac accacagaaa ttttacctca cacctccaca agtcaatagc atccttaaag
481 ctaatgaata cagtttcaaa gtgccagaat ttgacggcaa aaatgtcagt tctatccttg
541 gatttgacag caatcagctg cctgcaaatg cacccattga ggaccggaga agtgcagcaa
601 cctgcttgca gaccagaggg atgcttttgg gggtttttga tggccatgca ggttgtgctt
661 gttcccaggc agtcagtgaa agactctttt attatattgc tgtctctttg ttaccccatg
721 agactttgct agagattgaa aatgcagtgg agagcggccg ggcactgcta cccattctcc
781 agtggcacaa gcaccccaat gattacttta gtaaggaggc atccaaattg tactttaaca
841 gcttgaggac ttactggcaa gagcttatag acctcaacac tggtgagtcg actgatattg
901 atgttaagga ggctctaatt aatgccttca agaggcttga taatgacatc tccttggagg
961 cgcaagttgg tgatcctaat tcttttctca actacctggt gcttcgagtg gcattttctg
1021 gagccactgc ttgtgtggcc catgtggatg gtgttgacct tcatgtggcc aatactggcg
1081 atagcagagc catgctgggt gtgcaggaag aggacggctc atggtcagca gtcacgctgt
1141 ctaatgacca caatgctcaa aatgaaagag aactagaacg gctgaaattg gaacatccaa
1201 agagtgaggc caagagtgtc gtgaaacagg atcggctgct tggcttgctg atgccattta
1261 gggcatttgg agatgtaaag ttcaaatgga gcattgacct tcaaaagaga gtgatagaat
1321 ctggcccaga ccagttgaat gacaatgaat ataccaagtt tattcctcct aattatcaca
1381 cacctcctta tctcactgct gagccagagg taacttacca ccgattaagg ccacaggata
1441 agtttctggt gttggctact gatgggttgt gggagactat gcataggcag gatgtggtta
1501 ggattgtggg tgagtaccta actggcatgc atcaccaaca gccaatagct gttggtggct
1561 acaaggtgac tctgggacag atgcatggcc ttttaacaga aaggagaacc aaaatgtcct
1621 cggtatttga ggatcagaac gcagcaaccc atctcattcg ccacgctgtg ggcaacaacg
1681 agtttgggac tgttgatcat gagcgcctct ctaaaatgct tagtcttcct gaagagcttg
1741 ctcgaatgta cagagatgac attacaatca ttgtagttca gttcaattct catgttgtag
1801 gggcgtatca aaaccaagaa tagtgagtgg ctctttcact ggcaattctc aaatgatata
1861 catttaaagg gcagattttt taaaaagata ctactataat aaacatttcc agttggtcat
1921 tctaagcatt tacccttttg atactctagc tagtcaggta ctccaaattg actttgcagc
1981 agggtggcag ggtcaggaga gtctggtcct gcctagctca gatttcatgg cacctgcact
2041 tgaagcaagt cacttcttta tcacaggtgt cttgaaacat tagcttcttt taccaacctg
2101 agaaaattag gatgacctgg caaataagat cttgaatagg ccaaaagcaa gtatcttgct
2161 gtgtgtagtc tcttggttaa agtgaagaaa cagtactgtt cacacctttc ttcactgaga
2221 ttccagtgta catgagaaca tatatttatt gcatgatttt ctagatacac agtctatgca
2281 ttattcatat acatttattt tagcctaaag tggttttcaa atccagttct tcaagccata
2341 aatgaccaag atccaagcaa tctgaatttg tttttgtgat tatttgactg gaatgcttct
2401 taagtggaat aactatactc cgttatccac ccgatttcct aatgtaattg aaagattttc
2461 tattttgcca cacacttgga gacaataagg gtttttagtt ttatctactc ttctattgaa
2521 gttaaaaaaa aaaaaaaa
//