LOCUS BC064977 1652 bp mRNA linear HUM 13-JAN-2004
DEFINITION Homo sapiens mitochondrial translational initiation factor 2, mRNA
(cDNA clone IMAGE:6153154), partial cds.
ACCESSION BC064977
VERSION BC064977.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1652)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1652)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2004) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 140 Row: k Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505276.
FEATURES Location/Qualifiers
source 1..1652
/db_xref="H-InvDB:HIT000261684"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6153154"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..>1652
/gene="MTIF2"
/db_xref="GeneID:4528"
/db_xref="MIM:603766"
CDS 257..>1652
/gene="MTIF2"
/codon_start=1
/product="MTIF2 protein"
/protein_id="AAH64977.1"
/db_xref="GeneID:4528"
/db_xref="MIM:603766"
/translation="MNQKLLKLENLLRFHTIYRQLHSLCQRRALRQWRHGFSSAYPVW
TAQLCAWPWPTDVLTGAALSQYRLLVTKKEEGPWKSQLSSTKSKKVVEVWIGMTIEEL
ARAMEKNTDYVYEALLNTDIDIDSLEADSHLDEVWIKEVITKAGMKLKWSKLKQDKVR
KNKDAVRRPQADPALLTPRSPVVTIMGHVDHGKTTLLDKFRKTQVAAVETGGITQHIG
AFLVSLPSGEKITFLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMKQTVESIQHA
KDAQVPIILAVNKCDKAEADPEKVKKELLAYDVVCEDYGGDVQAVPVSALTGDNLMAL
AEATVALAEMLELKADPNGPVEGTVIESFTDKGRGLVTTAIIQRGTLRKGSVLVAGKC
WAKVRLMFDENGKTIDEAYPSMPVGITGWRDLPSAGEEILEVESEPRAREVVDWRKYE
QEQEKGQEDLKKKKK"
misc_feature 791..1258
/gene="MTIF2"
/note="GTP_EFTU; Region: Elongation factor Tu GTP binding
domain. This domain contains a P-loop motif, also found in
several other families such as pfam00071, pfam00025 and
pfam00063. Elongation factor Tu consists of three
structural domains, this plus two C-terminal beta barrel
domains"
/db_xref="CDD:pfam00009"
BASE COUNT 532 a 297 c 418 g 405 t
ORIGIN
1 ccaggggccc ggggctgtag attccttgac aaggatatcc tagcggcgaa acaacaccgt
61 actgggagtc agaacgtctg ggttctagtc ttgactgcca ttaactagcg gtatgacatt
121 ggagaagctt ttttgaccct tctggatttc cgtttccttt tctgtaaaat gaggagcttg
181 gaagatccgg aaaatgaggc ccataggaaa caagtgactt gctgagtcca gataacactg
241 actgtcagag agaaacatga accagaagct actgaagttg gagaacttgc tacgatttca
301 cactatttat aggcaactgc acagtctgtg tcaaagaaga gcattaagac agtggaggca
361 tgggttttca tctgcttacc ctgtgtggac agctcaactg tgtgcctggc cctggccaac
421 agatgtgctc actggggctg ctttatctca gtataggctt ctagtaacaa aaaaggaaga
481 aggaccatgg aaatctcagt tatcttcaac aaaatctaaa aaggtggtag aagtatggat
541 tggaatgact attgaggaac tggccagggc aatggaaaaa aacacagatt atgtatatga
601 agctttattg aacactgata ttgacataga ttcactggaa gcagactcac atttagatga
661 agtctggatc aaagaagtga taacgaaggc agggatgaag ttaaagtgga gtaaattaaa
721 acaggacaaa gtcagaaaaa ataaagatgc tgtaagaagg ccccaggcag atccagcttt
781 attaacccca aggtccccag ttgttactat aatgggccat gttgatcacg ggaaaacgac
841 attacttgac aaatttcgaa aaactcaagt ggcagcagtg gaaactggag gcatcactca
901 gcacattggt gcctttcttg tctctctgcc ttctggggaa aagataactt ttcttgatac
961 tccaggacat gctgctttct cagcaatgag agccagaggt gctcaggtca ctgacattgt
1021 cgtattggtt gtagctgcag atgatggagt gatgaaacaa actgtagaat ctattcagca
1081 tgccaaagat gcacaggttc ctattatcct tgccgtaaat aaatgtgaca aagctgaggc
1141 tgatcctgag aaagtgaaaa aagagctgct ggcttacgat gtggtatgtg aagattatgg
1201 aggtgatgtt caagcagtgc ctgtctccgc acttacgggc gataatctga tggctttggc
1261 agaagcaaca gttgctcttg cagaaatgtt agaattgaaa gcagatccca atggtccagt
1321 ggaaggaaca gtaatagagt ctttcacaga caaaggaaga ggtcttgtta ctacagctat
1381 aattcaaaga ggaactttaa gaaaaggctc tgttctggtt gctggaaaat gttgggcaaa
1441 agtacgctta atgtttgatg aaaatggaaa aacaattgat gaggcctatc ccagcatgcc
1501 agtgggaatt acaggctgga gagaccttcc ttctgcagga gaagaaattc ttgaagtaga
1561 atctgagcca agggcacgtg aagttgttga ctggaggaaa tatgaacaag aacaggagaa
1621 aggtcaggag gatctgaaaa aaaaaaaaaa aa
//