LOCUS BC064638 5271 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens platelet-activating factor acetylhydrolase, isoform
Ib, alpha subunit 45kDa, mRNA (cDNA clone MGC:70757 IMAGE:5786560),
complete cds.
ACCESSION BC064638
VERSION BC064638.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5271)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 5271)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 139 Row: p Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6031206.
FEATURES Location/Qualifiers
source 1..5271
/db_xref="H-InvDB:HIT000261557"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70757 IMAGE:5786560"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_71"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..5271
/gene="PAFAH1B1"
/gene_synonym="LIS1"
/gene_synonym="LIS2"
/gene_synonym="MDCR"
/gene_synonym="PAFAH"
/db_xref="GeneID:5048"
/db_xref="HGNC:HGNC:8574"
/db_xref="MIM:601545"
CDS 211..1443
/gene="PAFAH1B1"
/gene_synonym="LIS1"
/gene_synonym="LIS2"
/gene_synonym="MDCR"
/gene_synonym="PAFAH"
/codon_start=1
/product="platelet-activating factor acetylhydrolase,
isoform Ib, alpha subunit 45kDa"
/protein_id="AAH64638.1"
/db_xref="GeneID:5048"
/db_xref="HGNC:HGNC:8574"
/db_xref="MIM:601545"
/translation="MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELD
KKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKY
ALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDH
SGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKM
WEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHE
HVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTL
VGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTA
PYVVTGSVDQTVKVWECR"
BASE COUNT 1523 a 961 c 1141 g 1646 t
ORIGIN
1 ggcggcggtg cagcgctccg gtggaatgaa tcttacttgt tgaatatctt ctggttacta
61 gttggattca tttgtgaaag aatcattttc ccctgtgtgg aagacactta gtggcatatt
121 taaattataa gtccacggat caaaaagctt tttgatttcc caaaggaggg acataccact
181 atatcagata agcttgacat tacagccaag atggtgctgt cccagagaca acgagatgaa
241 ctaaatcgag ctatagcaga ttatcttcgt tcaaatggct atgaagaggc atattcagtt
301 tttaaaaagg aagctgaatt agatgtgaat gaagaattag ataaaaagta tgctggtctt
361 ttggaaaaaa aatggacatc tgttattaga ttacaaaaga aggttatgga attagaatca
421 aagctaaatg aagcaaaaga agaatttacg tcaggtggac ctcttggtca gaaacgagac
481 ccaaaagaat ggattccccg tccgccagaa aaatatgcat tgagtggtca caggagtcca
541 gtcactcgag tcattttcca tcctgtgttc agtgttatgg tctctgcttc agaggatgct
601 acaattaagg tgtgggatta tgagactgga gattttgaac gaactcttaa aggacataca
661 gactctgtac aggacatttc attcgaccac agcggcaagc ttctggcttc ctgttctgca
721 gatatgacca ttaaactatg ggattttcag ggctttgaat gcatcagaac catgcacggc
781 catgaccaca atgtttcttc agtagccatc atgcccaatg gagatcatat agtgtctgcc
841 tcaagggata aaactataaa aatgtgggaa gtgcaaactg gctactgtgt gaagacattc
901 acaggacaca gagaatgggt acgtatggta cggccaaatc aagatggcac tctgatagcc
961 agctgttcca atgaccagac tgtgcgtgta tgggtcgtag caacaaagga atgcaaggct
1021 gagctccgag agcatgagca tgtggtagaa tgcatttcct gggctccaga aagctcatat
1081 tcctccatct ctgaagcaac aggatctgag actaaaaaaa gtggtaaacc tgggccattc
1141 ttgctgtctg gatccagaga caagactatt aagatgtggg atgtcagtac tggcatgtgc
1201 cttatgaccc tcgtgggtca tgataactgg gtacgtggag ttctgttcca ttctgggggg
1261 aagtttattt tgagttgtgc tgatgacaag accctacgcg tatgggatta caagaacaag
1321 cgatgcatga agaccctcaa tgcgcatgaa cactttgtta cctccttgga tttccacaag
1381 acggcaccct atgtcgtcac tggcagcgta gatcaaacag taaaagtgtg ggagtgccgt
1441 tgattgtgtc tccttcagcc cctcctccct cttttcctct ggatgcactc tgatgatacc
1501 atggttaccc cattgagctc tgtttaaata aatattgtcc tttcatgtaa attattctgg
1561 atgtagattg agcttattaa atgttacaca caaagtattc atgcatggtg aatccaaatt
1621 gtatactgta aatttacata cgttgtctag aagtaccata gggtttaaaa acctgggctg
1681 gcattggtca caccaggcct aagaaggcag aagttgaatc aattgaacta gggcactaaa
1741 ctgaatagtt gacagtgtca ttttatgttg gattattaat tcctgttttt ctttctgcta
1801 tctgttggtg cctgacttga tggcctcatt tggggaaaag tggtggttat tagggctttt
1861 tctgaaatgt gtatctatgt aacatcactt aagtgtgctt aataaatctt ctgtaaggat
1921 tttagatgat aaggctacaa ttcagaatct tctgaaccat ctatgtaatg aatggggatt
1981 atacattgga atttttgtca tgacacattt gccaaatcag taggatatat ttgttttggc
2041 agcctatcac gcagaggcta gtggtatatt tatgtaagaa aatgactgta aatctcaaga
2101 aaaatctcag cagctaatag caactcattt atttcatttt ggtcttaatg ctttgtaaac
2161 aggtcaaaaa atactgtcat actctaagct tctattttcc acactggaca tacttctagt
2221 tgtattctcc atactattag actgtgtagt gatgtgactt ccaagtagaa tttaatctcc
2281 ccattgagtg tgtcatggta caaatcacta ttcgtttttg gtgtttttta gggatgtgca
2341 atgtgcatta cataatgaca gaaatactga gaaggttctg tgtgcccatt tgaaaggagt
2401 gggaggaata cagcagtttg tttttcaaca tgaatctgat attgatttaa actgtgtttc
2461 acttacaagt tttaaaaaaa tgacagggtt taatggagcg tgcataaaaa tgtactgttt
2521 tcaccttttg tttatatgta aatgtttgta agtatatggg cctatctgta agtgggtaag
2581 tctgtatgtg tgtatcatac acatcaacct ccatgtcctt agtcctgggt ttttgaaaaa
2641 gtgctaaaac ggacaagtag aataaatgtt gctgtggaat gccatgcttt agaacaaacc
2701 ctttttgatc ttaatgcttc tgaaaactag gtctgactct ggggattttt ttccagccga
2761 aggaaaatca cttccgttat gtccccctct aatttagccg ctcgacattt tacacaaccc
2821 ggatatgttg tatattttga cccaaagtta caggtaggtt taagagaatt tttagccatg
2881 acttttggag cactattcca ttgtcagtta ttaataaaga attccattgc ttagctaacc
2941 aacaggtttt ttttgtttcc aagagagtta tttgaaaagt taacagaaca atgagataac
3001 agtgacagtt taacaaagat aaaattctga actgcgtttt attcatttgt gtactatgtg
3061 attttttaaa tgtccccttt agtatttaat ggaaaattgg ttcctgcaaa agacaaaggg
3121 tgagagttag cgtcctgtag atacacacag agactaggcc gtatattaac tagaagcagc
3181 tttatgtcta gcttgtgtct ttttgtttgt ttgcttgttt gtttttagat tcctgagaga
3241 tgtctctgga agggaaagtt ttgagaacta atggctattt ttgaggacaa aaattacatc
3301 ttaagctaat tccttaaata catacagtag gtgaattttc aggacaatat tgcctcacaa
3361 ccctgcttac attgaaaagt ctttttccct tagctcttct gactggattt ttctacaaaa
3421 actatggaaa atatctttgt tcttgtttgc tgctattttc tgtcctattt tgagaaatat
3481 aaatacatag aaatggtgca tcttaacatt tgtttgtaca tgtataaatg tcttgtattt
3541 taattcattt ttagcatgaa ttgtttaagg gtaagccaca acatctagaa atcactcata
3601 gatattgaac aataaaggag aatggtaccg atgcaggagg aagcaagcgt gtcttcccct
3661 gcagcacaca gcgacttgcg ttgacaaagg aggaggaaac gattactctg taaacaaagt
3721 tatccttact tgggagattg ccacagcctg ctgctgagtt gagttaccag acatcctcca
3781 tgtgagaagc agcgaacatt gaatctcagg gatggcccac aactgggtcc acatgtaatg
3841 agccctgttt aataacgaag gggtggggga gagcagtccg tctacaacct ggaatcagat
3901 ttgcaaaaat ttcctgcact gctgtctgac actgtcctgt tgatgccctt tctgactgtg
3961 ttctctgttt tctctgtctg ctgtctaacc ctgtgccttg cctgggataa ggacaatgat
4021 gaggttactg gtttggattg taagtagagg acttttatta attggtttag aggttcactg
4081 ctgctttgtc actttctcaa tcaaattggc cacttaagaa ataaagagct ggtagaattg
4141 catcctcaga tgattattga ctgtgtgtgt gtgtgaaaac agacattcca gtgccaccca
4201 aatatatatc tgtaacgtgc ccaagaaatc ctagctgcgc tcttgagagt gcatgccatg
4261 gagactggtt tagacaccgc gtggagccta gttgcctgtt gtcacggcat cttgcacttt
4321 aggagactaa gaccgtcctg gttcgtctgt gtgtggtgtg accaatggtg tgcccagagc
4381 actactctca aaatcactag tgttagcaag tcgtcccggg ctggggagcg ttcgccgtag
4441 tctttggaag ctttggcttt agatttacca agccccgcct ccccgctgcc agtgccctgc
4501 tctcccgttc gcctctttct gtttctgtgt gaactttccc ggtaatatca ctcgttaaat
4561 aggttttctt taaacttaat taaagaaaaa ctatttaaag gtaaaggata ttttgttgac
4621 atcggtggct cgatcatcct taagcaactg aagttaaaat tgttgaagga aaaggcactt
4681 aaattggtta ctttcatgtc cagctgtata taagtccagt gtgttcatct agatgacgca
4741 aagaatctcc tggtagagaa gcgacatgta aaaaactggt ggaaaaaggt tttggatttt
4801 tttccagtgg ggtgggggga gggcaagctg gatttacagg tcacggctgg actgaatggg
4861 cctttttatc ttcccactgt atcatggaag tagctgcttg cttgtactgt ccatccttca
4921 ggcatcccta aagctcactc tgaagatgtt agagacaaac acaaactctt cgagttaaag
4981 ttgatcctga cactgacatg aaggcaagcc ttgatttcgt atgaacgttg ctgaagtggt
5041 aattgaggaa aacagttccc cagattgtta agagttcact gaagatattg acacaatttt
5101 aaaaaatcag taaaggaatg tatataatat tgctctcgtg ttttacagta agatttgttg
5161 ctctcagact gtgtaaaaca aaatttattc atgttttctg catattaaaa aatcttattg
5221 taccaactgg taaactatta aatgcctata aaactaaaaa aaaaaaaaaa a
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