LOCUS BC064420 2530 bp mRNA linear HUM 09-JUN-2008
DEFINITION Homo sapiens myosin light chain kinase, mRNA (cDNA clone
IMAGE:5751596), complete cds.
ACCESSION BC064420
VERSION BC064420.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2530)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2530)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 139 Row: m Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 47132572
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..2530
/db_xref="H-InvDB:HIT000261383"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5751596"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2530
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
CDS 240..701
/gene="MYLK"
/gene_synonym="KRP"
/gene_synonym="MLCK"
/gene_synonym="MLCK1"
/gene_synonym="MLCK108"
/gene_synonym="MLCK210"
/gene_synonym="MSTP083"
/gene_synonym="MYLK1"
/gene_synonym="smMLCK"
/codon_start=1
/product="myosin light chain kinase"
/protein_id="AAH64420.2"
/db_xref="GeneID:4638"
/db_xref="HGNC:HGNC:7590"
/db_xref="MIM:600922"
/translation="MAMISGLSGRKSSTGSPTSPLNAEKLESEDVSQAFLEAVAEEKP
HVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDG
NCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE"
BASE COUNT 735 a 514 c 502 g 779 t
ORIGIN
1 catcttctga cacactgaca gtgctgagca gagcaaggtt gggttcgctc ctctggcaga
61 acctcggctc tcaggaggtc cttgttccag ggaacagctg cttctctggg gctgggctct
121 actccctgca gcccctcgca ctacccagct ggaaccaggg acaacgcctg agtccaaccc
181 tcgtgtctat tttccagaaa acgggcaatg ctgtgagagc cattggaaga ctgtcctcta
241 tggcaatgat ctcagggctc agtggcagga aatcctcaac agggtcacca accagcccgc
301 tcaatgcaga aaaactagaa tctgaagatg tgtcccaagc tttccttgag gctgttgctg
361 aggaaaagcc tcatgtaaaa ccctatttct ctaagaccat tcgcgattta gaagttgtgg
421 agggaagtgc tgctagattt gactgcaaga ttgaaggata cccagacccc gaggttgtct
481 ggttcaaaga tgaccagtca atcagggagt cccgccactt ccagatagac tacgatgagg
541 acgggaactg ctctttaatt attagtgatg tttgcgggga tgacgatgcc aagtacacct
601 gcaaggctgt caacagtctt ggagaagcca cctgcacagc agagctcatt gtggaaacga
661 tggaggaagg tgaaggggaa ggggaagagg aagaagagtg aaacaaagcc agagaaaagc
721 agtttctaag tcatattaaa aggactattt ctctaaaact caaaaaaaaa aaaaaactca
781 agatagtaaa agcacctagt gtgatagatt atcggttagg tcatttgtgg gttgattctt
841 cagaaacagc agttgatacc tagcagcgtt attgatgggc attaatctat gttagttggc
901 accttaagat actagtgcag ctagatttca tttagggaaa tcaccagtaa cttgactgac
961 caattgattt tagagagaaa gtaaccaaac caaatattta tctgggcaaa gtcataaatt
1021 ctccacttga atgcgctcat gaaaaataag gccaaaacaa gagttctggg ccacagctca
1081 gcccagaggg ttcctgggga tgggacgcct ctctctcccc accccctgac tctagagaac
1141 tgggttttct cccagtactc cagcaattca tttctgaaag cagttgagcc actttattcc
1201 aaagtacact gcagatgttc aaactctcca tttctctttc cccttccacc tgccagtttt
1261 gctgactctc aacttgtcat gagtgtaagc attaaggaca ttatgcttct tagattctga
1321 agacaggtcc ctgctcatgg atgactctgg cttccttagg aaaatatttt tcttccaaaa
1381 tcagtaggaa atctaaactt atcccctctt tgcagatgtc tagcagcttc agacatttgg
1441 ttaagaaccc atgggaaaaa aaaaatcctt gctaatgtgg tttcctttgt aaaccaggat
1501 tcttatttgt gctgttatag aatatcagct ctgaacgtgt ggtaaagatt tttgtgtttg
1561 aatataggag aaatcagttt gctgaaaagt tagtcttaat tatctattgg ccacgatgaa
1621 acagatttca actgataaag agctggagaa ctccatgtac tttggaatct cctccaagat
1681 agccagagtt taatacatct tcattctcaa cactctccaa agaacttgac ctaccttatg
1741 ggttccatat ttttcttctt aaatgtgcat caatcatgcc ttgcccccaa cctttaaata
1801 tattcttaga cctggtaaat gcactcagac ttgcgtcttt aggaattttt aactttcttt
1861 cactacattg gcactaaatt ttttctttat aaagcttttt gaaggtcata aacaaagacc
1921 ataattgatg atagacctaa tacatttcct ctgtgtgtgt gtgtaacatt ccaaatactt
1981 tttttttctt ttccactgtt tgtaaggtgc aacaatttaa tatttttaag ggacttttta
2041 agagttcctt aagaaccaat ttaaaattac ttcagtgcaa tcctacacag tatcaacatt
2101 agaattttga tattagtctt atgttatctt ccattctatt tttatctgct ttttgctgct
2161 agtttcaaac tgccagtatt tttccttttg cttttaaaat agttacaata tttttcatga
2221 tagccacagt attgccacag tttattataa taaagggttt ttatttgatt tagcgcattc
2281 aaagcttttt tctatcactt ttgtgttcag aatataacct ttgtgtgcgt gtatgttgtg
2341 tgtgtgcatg tgtggcgtat atgtgtgtta caggttaatg ccttcttgga attgtgttaa
2401 tgttctcttg gtttattatg ccatcagaat ggtaaatgag aacactacaa ctgtagtcag
2461 ctcacaattt ttaaataaag gataccacag tgcatgctgt ttgttcaaaa aaaaaaaaaa
2521 aaaaaaaaaa
//