LOCUS BC064374 1520 bp mRNA linear HUM 06-APR-2004
DEFINITION Homo sapiens roundabout, axon guidance receptor, homolog 2
(Drosophila), mRNA (cDNA clone IMAGE:5722845), partial cds.
ACCESSION BC064374
VERSION BC064374.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1520)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1520)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (15-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Invitrogen
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 139 Row: g Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1520
/db_xref="H-InvDB:HIT000261337"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5722845"
/tissue_type="Ovary, pooled from 3 adults"
/clone_lib="NIH_MGC_125"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1520
/gene="ROBO2"
/gene_synonym="KIAA1568"
/db_xref="GeneID:6092"
/db_xref="MIM:602431"
CDS <1..1028
/gene="ROBO2"
/gene_synonym="KIAA1568"
/codon_start=3
/product="ROBO2 protein"
/protein_id="AAH64374.1"
/db_xref="GeneID:6092"
/db_xref="MIM:602431"
/translation="NNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRK
GLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIG
NFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILH
SNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQ
KNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLED
ELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQF
DIAKQTW"
BASE COUNT 553 a 312 c 296 g 359 t
ORIGIN
1 acaataacag cataactgag caaatcactg atgtggtgaa gcaaccagcc tttatagctg
61 gtattggtgg tgcctgctgg gtaattctga tgggttttag catatggttg tattggcgaa
121 gaaagaagag gaagggactc agtaattatg ctgttacgtt tcaaagagga gatggaggac
181 taatgagcaa tggaagccgt ccaggtcttc tcaatgctgg tgatcccagc tatccatggc
241 ttgctgattc ttggccagcc acgagcttgc cagtaaataa tagcaacagt ggcccaaatg
301 agattggaaa ttttggccgt ggagatgtgc tgccaccagt tccaggccaa ggggataaaa
361 cagcaacgat gctctcagat ggagccattt atagtagcat tgacttcact accaaaacca
421 gttacaacag ttccagccaa ataacacagg ctaccccata tgccacgaca cagatcttgc
481 attccaacag catacatgaa ttggctgtcg atctgcctga tccacaatgg aaaagctcaa
541 ttcagcaaaa aacagatctg atgggatttg gttattctct acctgatcag aacaaaggta
601 acaatggtgg gaaaggtgga aaaaagaaga aaaataaaaa ctcttctaaa ccacagaaaa
661 acaatggatc cacttgggcc aatgtccctc tacctccccc cccagtccag ccccttcctg
721 gcacggagct ggaacactat gcagtggaac aacaagaaaa tggctatgac agtgatagct
781 ggtgcccacc attgccagta caaacttact tacaccaagg tctggaagat gaactggaag
841 aagatgatga tagggtccca acacctcctg ttcgaggcgt ggcttcttct cctgctatct
901 cctttggaca gcagtccact gcaactctta ctccatcccc acgggaagag atgcaaccca
961 tgctgcaggc tcacctggat gagttgacaa gagcctatca gtttgatata gcaaaacaaa
1021 catggtaaat atcttcatta agtcaagaga cccatgcttt caacacatgg aaattttttt
1081 cagaataaat tcacaagaga ttctgataga actacacagc tagggttaaa agtgattttg
1141 tataactcct taatcattta gataaatgta tccactgaga aatgttaaat aatttggcca
1201 aaactagaac tagaactaga actagaactg gaactggagc tgtaactgta actgtaacta
1261 gaactagaac taaaactaga actagagcaa gaattcaaat ctgctgtatt tcaggctaaa
1321 gtgctcttgg ctacaccaga atcttttcta atttatggtt tatcttcaat tagcttatca
1381 ttgaactcac atcccatgat gtaataaaag actcctttta gattcctcaa aaaaaaaaaa
1441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1501 aaaaaaaaaa aaaaaaaaaa
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