LOCUS BC064357 3485 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol binding clathrin assembly
protein, mRNA (cDNA clone MGC:70760 IMAGE:5112878), complete cds.
ACCESSION BC064357
VERSION BC064357.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3485)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3485)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: p Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 56788365.
FEATURES Location/Qualifiers
source 1..3485
/db_xref="H-InvDB:HIT000261320"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70760 IMAGE:5112878"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3485
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
CDS 280..2112
/gene="PICALM"
/gene_synonym="CALM"
/gene_synonym="CLTH"
/gene_synonym="LAP"
/codon_start=1
/product="phosphatidylinositol binding clathrin assembly
protein"
/protein_id="AAH64357.1"
/db_xref="GeneID:8301"
/db_xref="HGNC:HGNC:15514"
/db_xref="MIM:603025"
/translation="MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDY
LIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNT
LFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMN
TEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINL
LEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLD
ALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQAR
LKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPND
LLDLQQPTFHPSVHPMSTASQVASTWGGFTPSPVAQPHPSAGLNVDFESVFGNKSTNV
IVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTT
KNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMNGMHFPQYAPPVMAYPATTPTGM
IGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM"
BASE COUNT 1055 a 705 c 750 g 975 t
ORIGIN
1 ctcttaccct gcccggctcc agctgaccag ggaaggggtg ggctgaactg aggcgggggc
61 aagggagtgc ccgacatctt gtccgactcc gcgggtgaca cgagccggtt ctctctggac
121 tggtggcagc gcgcggcccc gaaccgcgcc ccaggccggc aggcggggaa ggagccggtg
181 ggggtagggg gtgcggtggg gggtggggac cctccggctc ttgggggtcc cagtccccgc
241 cggctgctga gcgggtgggg tggtggagga gctgcagaga tgtccggcca gagcctgacg
301 gaccgaatca ctgccgccca gcacagtgtc accggctctg ccgtatccaa gacagtatgc
361 aaggccacga cccacgagat catggggccc aagaaaaagc acctggacta cttaattcag
421 tgcacaaatg agatgaatgt gaacatccca cagttggcag acagtttatt tgaaagaact
481 actaatagta gttgggtggt ggtcttcaaa tctctcatta caactcatca tttgatggtg
541 tatggaaatg agcgttttat tcagtatttg gcttcaagaa acacgttgtt taacttaagc
601 aattttttgg ataaaagtgg attgcaagga tatgacatgt ctacatttat taggcggtat
661 agtagatatt taaatgagaa agcagtttca tacagacaag ttgcatttga tttcacaaaa
721 gtgaagagag gggctgatgg agttatgaga acaatgaaca cagaaaaact cctaaaaact
781 gtaccaatta ttcagaatca aatggatgca cttcttgatt ttaatgttaa tagcaatgaa
841 cttacaaatg gggtaataaa tgctgccttc atgctcctgt tcaaagatgc cattagactg
901 tttgcagcat acaatgaagg aattattaat ttgttggaaa aatattttga tatgaaaaag
961 aaccaatgca aagaaggtct tgacatctat aagaagttcc taactaggat gacaagaatc
1021 tcagagttcc tcaaagttgc agagcaagtt ggaattgaca gaggtgatat accagacctt
1081 tcacaggccc ctagcagtct tcttgatgct ttggaacaac atttagcttc cttggaagga
1141 aagaaaatca aagattctac agctgcaagc agggcaacta cactttccaa tgcagtgtct
1201 tccctggcaa gcactggtct atctctgacc aaagtggatg aaagggaaaa gcaggcagca
1261 ttagaggaag aacaggcacg tttgaaagct ttaaaggaac agcgcctaaa agaacttgca
1321 aagaaacctc atacctcttt aacaactgca gcctctcctg tatccacctc agcaggaggg
1381 ataatgactg caccagccat tgacatattt tctaccccta gttcttctaa cagcacatca
1441 aagctgccca atgatctgct tgatttgcag cagccaactt ttcacccatc tgtacatcct
1501 atgtcaactg cttctcaggt agcaagtaca tggggaggat tcactccttc tccagttgca
1561 cagccacacc cttcagctgg ccttaatgtt gactttgaat ctgtgtttgg aaataaatct
1621 acaaatgtta ttgtagattc tgggggcttt gatgaactag gtggacttct caaaccaaca
1681 gtggcctctc agaaccagaa ccttcctgtt gccaaactcc cacctagcaa gttagtatct
1741 gatgacttgg attcatcttt agccaacctt gtgggcaatc ttggcatcgg aaatggaacc
1801 actaagaatg atgtaaattg gagtcaacca ggtgaaaaga agttaactgg gggatctaac
1861 tggcaaccaa aggttgcacc aacaactgct tggaatgctg caacaatgaa tggcatgcat
1921 tttccacaat acgcaccccc tgtaatggcc tatcctgcta ctacaccaac aggcatgata
1981 ggatatggaa ttcctccaca aatgggaagt gttcctgtaa tgacgcaacc aaccttaata
2041 tacagccagc ctgtcatgag acctccaaac ccctttggcc ctgtatcagg agcacagata
2101 cagtttatgt aacttgatgg aagaaaatgg aattactcca aaaagacaag tgctcaagca
2161 gcaaaatcct tacttccagc aaaatccaaa ctgctgtctc ttaaatctct taaactctct
2221 tcttccatta gaatgctaca agtaactcag tgaaggccca tgaaggaaat tgggactagt
2281 ttataggaga acgtatcaat acagtttata aagccaagaa ttgctatgat ttaagactaa
2341 gatctgtctt tttggtgact aacccttcaa ttctttcaac tcctgttaat acccataatc
2401 agtaacctat caagaaaagc ccttatttgg aaagtgtgaa atttgtattt ggaaaagctg
2461 cctggagaga agaactgtgt cctttactgt atttcaacag gactcttttg ggggatcaaa
2521 attaaaattc ctaattatgc attatctttc ttttctccag tcctcacaaa tacagaaaca
2581 ataactgaaa ttaacttttc tttttttaaa aaaaattata ttcagtttgc agtagacatt
2641 ccttaagtat ttgtatttat ttatgattat caattttaca taacattaat attgtatcag
2701 acctccttat gaaaatgagt atggatgtgc acagtatgtt tgatttttat ccacaagaat
2761 gaatctgatt cagaatgctt ttctcagctg acatacagag cactaaatat tttaaggcaa
2821 gtccataggt ctgaatctct taagaattct cggcctctgt gggatttagg gaagcattat
2881 aaatgcatta atccttatag tcaattctgt gcctaggatt ttgccaggga acagttcact
2941 gactaggaaa agcactacat tttaaattca gcattagtgc attgggaagg atctttactg
3001 ctttgtgctt ggcatgtcat tattttccat ttgacattag ggcctttcca aaatgaatgt
3061 gaggaattgc tttcacttca agactttcct tcttttcact aaaactctag aaggtgttac
3121 aagggggagg gaaggggggc aaagtccttg aacattttct ttggctcgtg ccatgttatg
3181 atcatatacc ttttaaataa ggggaaatag tatctttaaa gttaatgtct agccaagagt
3241 ttagtaaacg aagaattaaa ctgcactgtt gatcggtgct ttgtgtaaat acatctttaa
3301 catttgggtg gagaggggcc ttaagaagga cagttcattg taggaaagca attctgtaca
3361 tgagtttaag cattcttgtt gcattgtctc tgcagattct atttttgttt acaatattaa
3421 aatgtatgtt agcaaaatgg gtggattttc aaataaaatg cagcttccac aaaaaaaaaa
3481 aaaaa
//