LOCUS BC063511 1182 bp mRNA linear HUM 02-JAN-2004
DEFINITION Homo sapiens eukaryotic translation elongation factor 1 alpha 1,
mRNA (cDNA clone IMAGE:3877674), partial cds.
ACCESSION BC063511
VERSION BC063511.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1182)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1182)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: c Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1182
/db_xref="H-InvDB:HIT000260969"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3877674"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_68"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..1182
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/db_xref="GeneID:1915"
/db_xref="MIM:130590"
CDS <1..875
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/codon_start=3
/product="EEF1A1 protein"
/protein_id="AAH63511.1"
/db_xref="GeneID:1915"
/db_xref="MIM:130590"
/translation="EVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKV
TRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKP
GMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKND
PPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDG
PKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGA
GKVTKSAQKAQKAK"
misc_feature 3..200
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/note="GTP_EFTU; Region: Elongation factor Tu GTP binding
domain. This domain contains a P-loop motif, also found in
several other families such as pfam00071, pfam00025 and
pfam00063. Elongation factor Tu consists of three
structural domains, this plus two C-terminal beta barrel
domains"
/db_xref="CDD:pfam00009"
misc_feature 264..467
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/note="GTP_EFTU_D2; Region: Elongation factor Tu domain 2.
Elongation factor Tu consists of three structural domains,
this is the second domain. This domain adopts a beta
barrel structure. This the second domain is involved in
binding to charged tRNA. This domain is also found in
other proteins such as elongation factor G and translation
initiation factor IF-2. This domain is structurally
related to pfam03143, and in fact has weak sequence
matches to this domain"
/db_xref="CDD:pfam03144"
misc_feature 483..782
/gene="EEF1A1"
/gene_synonym="EEF-1"
/gene_synonym="EEF1A"
/gene_synonym="eEF1A-1"
/gene_synonym="EEF1A1L14"
/gene_synonym="EF-Tu"
/gene_synonym="EF1A"
/gene_synonym="GRAF-1EF"
/gene_synonym="MGC16224"
/gene_synonym="PTI-1"
/gene_synonym="PTI1"
/note="GTP_EFTU_D3; Region: Elongation factor Tu
C-terminal domain. Elongation factor Tu consists of three
structural domains, this is the third domain. This domain
adopts a beta barrel structure. This the third domain is
involved in binding to both charged tRNA and binding to
EF-Ts pfam00889"
/db_xref="CDD:pfam03143"
BASE COUNT 331 a 247 c 288 g 316 t
ORIGIN
1 aggaagtcag cacttacatt aagaaaattg gctacaaccc cgacacagta gcatttgtgc
61 caatttctgg ttggaatggt gacaacatgc tggagccaag tgctaacatg ccttggttca
121 agggatggaa agtcacccgt aaggatggca atgccagtgg aaccacgctg cttgaggctc
181 tggactgcat cctaccacca actcgtccaa ctgacaagcc cttgcgcctg cctctccagg
241 atgtctacaa aattggtggt attggtactg ttcctgttgg ccgagtggag actggtgttc
301 tcaaacccgg tatggtggtc acctttgctc cagtcaacgt tacaacggaa gtaaaatctg
361 tcgaaatgca ccatgaagct ttgagtgaag ctcttcctgg ggacaatgtg ggcttcaatg
421 tcaagaatgt gtctgtcaag gatgttcgtc gtggcaacgt tgctggtgac agcaaaaatg
481 acccaccaat ggaagcagct ggcttcactg ctcaggtgat tatcctgaac catccaggcc
541 aaataagcgc cggctatgcc cctgtattgg attgccacac ggctcacatt gcatgcaagt
601 ttgctgagct gaaggaaaag attgatcgcc gttctggtaa aaagctggaa gatggcccta
661 aattcttgaa gtctggtgat gctgccattg ttgatatggt tcctggcaag cccatgtgtg
721 ttgagagctt ctcagactat ccacctttgg gtcgctttgc tgttcgtgat atgagacaga
781 cagttgcggt gggtgtcatc aaagcagtgg acaagaaggc tgctggagct ggcaaggtca
841 ccaagtctgc ccagaaagct cagaaggcta aatgaatatt atccctaata cctgccaccc
901 cactcttaat cagtggtgga agaacggtct cagaactgtt tgtttcaatt ggccatttaa
961 gtttagtagt aaaagactgg ttaatgataa caatgcatcg taaaaccttc agaaggaaag
1021 gagaatgttt tgtggaccac tttggttttc ttttttgcgt gtggcagttt taagttatta
1081 gtttttaaaa tcagtacttt ttaatggaaa caacttgacc aaaaatttgt cacagaattt
1141 tgagacccat taaaaaagtt aaatgagaaa aaaaaaaaaa aa
//