LOCUS BC063418 3436 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens kelch-like 20 (Drosophila), mRNA (cDNA clone MGC:74874
IMAGE:5221643), complete cds.
ACCESSION BC063418
VERSION BC063418.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3436)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3436)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 135 Row: j Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40807499.
FEATURES Location/Qualifiers
source 1..3436
/db_xref="H-InvDB:HIT000260878"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:74874 IMAGE:5221643"
/tissue_type="Pancreas, Spleen, adult pooled"
/clone_lib="NIH_MGC_120"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3436
/gene="KLHL20"
/gene_synonym="KHLHX"
/gene_synonym="KLEIP"
/gene_synonym="KLHLX"
/gene_synonym="RP3-383J4.3"
/db_xref="GeneID:27252"
/db_xref="HGNC:HGNC:25056"
CDS 110..1939
/gene="KLHL20"
/gene_synonym="KHLHX"
/gene_synonym="KLEIP"
/gene_synonym="KLHLX"
/gene_synonym="RP3-383J4.3"
/codon_start=1
/product="kelch-like 20 (Drosophila)"
/protein_id="AAH63418.1"
/db_xref="GeneID:27252"
/db_xref="HGNC:HGNC:25056"
/translation="MEGKPMRRCTNIRPGETGMDVTSRCTLGDPNKLPEGVPQPARMP
YISDKHPRQTLEVINLLRKHRELCDVVLVVGAKKIYAHRVILSACSPYFRAMFTGELA
ESRQTEVVIRDIDERAMELLIDFAYTSQITVEEGNVQTLLPAACLLQLAEIQEACCEF
LKRQLDPSNCLGIRAFADTHSCRELLRIADKFTQHNFQEVMESEEFMLLPANQLIDII
SSDELNVRSEEQVFNAVMAWVKYSIQERRPQLPQVLQHVRLPLLSPKFLVGTVGSDPL
IKSDEECRDLVDEAKNYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISS
VERYDPQTNEWRMVASMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQW
SSDVAPTSTCRTSVGVAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRR
LGVAVAVLGGFLYAVGGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDM
IYAVGGRDDTTELSSAERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTT
YLKTIEVFDPDANTWRLYGGMNYRRLGGGVGVIKMTHCESHIW"
BASE COUNT 1068 a 648 c 755 g 965 t
ORIGIN
1 gaggcagaga ggagtggagg gcggagtaga cggaggaggc tgctgcagag aagaaagtgt
61 cagagccggt tcggctttag agtgtggtga agggtacttt tcatggtgca tggaaggaaa
121 gccaatgcgc aggtgtacca acattcgacc aggagagact ggaatggatg taacaagccg
181 ctgcaccctt ggagacccca acaaactgcc agaaggggtt ccccaacctg cccgcatgcc
241 ctatatctca gacaagcacc ctcgacaaac cttggaagtg attaaccttc tgagaaagca
301 ccgggagcta tgtgatgtgg tgctagttgt gggcgccaag aagatatatg cccatcgagt
361 cattttgtca gcctgtagtc cctacttccg agctatgttt acaggagaat tggcagagag
421 ccgtcagaca gaagtagtga tccgagacat tgacgagagg gctatggaat tactgattga
481 ctttgcgtat acctcccaga taacagtaga agagggcaat gttcagactc ttctgccagc
541 tgcttgcctc ctccagctgg cagaaataca ggaagcctgc tgtgaattct taaagagaca
601 attagatcct tctaactgcc tgggcattcg ggcttttgct gacacacatt catgtcgtga
661 gttgctaagg atagcagaca agttcaccca acataacttt caagaggtaa tggagagtga
721 agagttcatg ttgcttccag ccaatcaact cattgatata atatccagtg atgagctaaa
781 cgttcgcagt gaagaacaag tgttcaatgc agtgatggcc tgggtcaaat acagtattca
841 ggaaagacgt cctcaattac cccaggtgct gcagcatgtt cgtttgcctt tgcttagtcc
901 caagttcctg gtcggcacag taggctctga tcccctcatc aaaagtgatg aagaatgcag
961 agacttggta gatgaggcta aaaactacct cctattgccg caagaacgac cactaatgca
1021 aggaccaagg acgagaccac ggaaacctat ccgatgtggg gaagtactct ttgcagttgg
1081 tggttggtgc agtggagatg ccatttccag tgttgaacga tatgatccac agaccaatga
1141 atggagaatg gtggcttcaa tgagcaaaag gagatgcgga gttggggtca gtgttcttga
1201 tgatctgtta tatgcagtag gaggccatga tggatcctct tatctcaata gtgttgaaag
1261 gtatgacccc aaaacaaacc agtggagcag tgatgtggcc cctacaagca cctgcaggac
1321 aagtgttggt gtagcagtac ttggaggctt tctttatgct gtgggtggcc aggatggtgt
1381 gtcttgcctc aacattgttg agaggtatga tccgaaggag aacaagtgga ctcgggtagc
1441 ttctatgagt accagaagac taggtgtggc tgtggctgtg ttaggagggt tcttatatgc
1501 tgtaggtggc tctgacggga catctcctct caacacagtg gaacgttaca atcctcagga
1561 aaacagatgg cacactatag cccctatggg gacccggagg aaacacctag gctgtgcagt
1621 atatcaggac atgatctatg ctgtaggagg tagagatgac actacagagc tgagcagtgc
1681 tgagagatac aaccccagaa ccaaccagtg gtctccagtg gtggccatga catcacgccg
1741 tagtggagtt ggcctggcag tggtcaatgg acagctcatg gcagtaggag gttttgatgg
1801 cacaacatac ttgaagacca tagaagtttt tgatcctgat gccaatacat ggaggttata
1861 tggcgggatg aattaccgtc ggctaggggg tggcgtagga gttattaaaa tgacacattg
1921 tgaatcccat atttggtgaa cacagagaag acagtcttgt atatattcct ctgtattctg
1981 gggagctttg accttggagc tttgtacagc ttgagaaaac attagaacaa attttattat
2041 ttgccggtgc ctcaacaaat ggaaatacaa tccaatgaaa gtacttcacc tgcaagatgc
2101 acaataattt tcaactctgt gcagaagaat atttattttt ggttttaatt tatcatggtt
2161 ttttgttgtt ttcgttttga acttatcctt cctcccacaa aaaaagaaaa gaagaaaaaa
2221 ttccaagcag caaaacttac tttgtttgta aggtattcat ttaggtttga aaatactatt
2281 taataagggc agaagggcta tatatgattt ggctattatt tctagacact ccatccacat
2341 gattccacta acaaggatta ccaggaataa aggtaggtat gcaaaatgta ttagctaccc
2401 attattctcg cactaaccac cagaagactt gaaaatctta aaaaaaaaaa aacaaaaaaa
2461 caagaaaagg caacatctca ttttaaatag tacaattcag aagggatact aaatcaataa
2521 aaaccaggac tcagacacta cagttttcat cagtgtaatt ttatgtcttg tttctttcta
2581 tatgaacttg tttatttagt ctttttttca taatattctc atagagtttc tgctagatct
2641 agagctctgc tcagtgcctc ttataaaaac aatatataag tctcatatgc tgtcttgaga
2701 atccaagaga atcacatgca gctctgcaac agtttttgct caaaaatgtt actgactaaa
2761 gcaactgtcc ttcctgtttc cttattgcta ccaaggacca gcagggagaa atgtcttctc
2821 ccagcagtga attctgttat gcaatttatt cttgatgctc aggcctggtt aagctgaggc
2881 ttacctttaa taacagcctc caagggaatg aattattcag ttaatgtaat agcacacatt
2941 aaagagtgta aaatcaattg aggcatttta ttaatatctt ggttctttta tacaaacaat
3001 gtatcattat cttcattcta taaggtcatt gttactactt ctctcaaatt gcactttctg
3061 gtaaaaagtt aaaaattttt aaggaaaaaa taatttcaat ggttaaagtt ttttttcctc
3121 attgtattca tagacaaatt tttttcaata tattttgcaa gaaaaatctt tgaataagac
3181 taactgcatt taaaaggaat aacctccttc tccctttccc caactacaaa aatgtttggc
3241 aactttgtgt ttacatttaa agatcatttg cacctttttc aaggaaaaaa agtattgagt
3301 aaacaattgt ttacatatat catttatgct tttttctagc atgtataact tttttaaata
3361 aaggtagtat ttaccattaa aaaaatttta gctaaaaaaa aaaaaaaaaa aaaaaaaaaa
3421 aaaaaaaaaa aaaaaa
//