LOCUS BC063117 2057 bp mRNA linear HUM 03-JAN-2005
DEFINITION Homo sapiens Rho guanine nucleotide exchange factor (GEF) 12, mRNA
(cDNA clone IMAGE:30353240), partial cds.
ACCESSION BC063117
VERSION BC063117.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2057)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2057)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Michael Brownstein and Dr. Miklos Palkovits
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 51 Row: b Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662087.
FEATURES Location/Qualifiers
source 1..2057
/db_xref="H-InvDB:HIT000260764"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:30353240"
/tissue_type="Glandular pool- thyroid, parathyroid,
adrenal cortex, pineal gland, submandibular gland."
/clone_lib="NIH_MGC_184"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..>2057
/gene="ARHGEF12"
/gene_synonym="KIAA0382"
/gene_synonym="LARG"
/gene_synonym="PRO2792"
/db_xref="GeneID:23365"
/db_xref="MIM:604763"
CDS 339..>2057
/gene="ARHGEF12"
/gene_synonym="KIAA0382"
/gene_synonym="LARG"
/gene_synonym="PRO2792"
/codon_start=1
/product="ARHGEF12 protein"
/protein_id="AAH63117.1"
/db_xref="GeneID:23365"
/db_xref="MIM:604763"
/translation="MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDF
DPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTL
VTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSP
GASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLK
EIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCS
SGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGA
EDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYK
HTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQ
ERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIE
EVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKK
KKKKKKK"
BASE COUNT 613 a 452 c 497 g 495 t
ORIGIN
1 ggggggagtc ccgggtcccc ttcccactgc gcgcggattt ccctctctga ggaagtttat
61 ccttgtgcct tctggaggat ttctctctag ctcgactcac tctggactga ctcgctccct
121 ggctttctca gttcgttttg ggagataact tgttttgctc caagccgcat ccgttgaccc
181 cttacagtcg gatggtctag atgactgaat ggagttttga gttggacttt tgtgtccctg
241 acggagttgg gcctgatccc agagcactgg gggtggggag gaggtgttac tgtaaaatgc
301 aagttggata aaaggaggac ctctcgccaa gggccccaat gagtggcaca cagtctacta
361 tcaccgacag gtttcccctc aaaaaaccta taaggcatgg aagtattttg aaccgagagt
421 caccaacaga taagaagcag aaagttgagc gcattgcatc acatgatttt gaccccacag
481 gtcttgttca gcgttgcgta atcatccaga aagatgacaa tggatttggg ctgacggtca
541 gtggagacaa tccagtcttc gtacagtctg tcaaagaaga tggagcagcc atgcgggctg
601 gagtacagac aggtgatcga atcatcaagg tgaatggaac tctggtgact cattcaaatc
661 atctggaggt ggtgaagcta atcaaatctg gttcctatgt agctctcact gttcagggac
721 gcccacctgg gtcgccccag attccacttg ccgactctga agtagagccg tcagtcattg
781 gacatatgtc tcccatcatg acatctcctc attcacctgg agcatctggg aatatggaga
841 gaatcactag tcctgtgctc atgggggagg aaaacaatgt ggttcataac cagaaagtag
901 aaattctgag aaaaatgtta cagaaagaac aggaacggct acagttattg caggaagatt
961 acaaccgaac acctgcccaa agattgctaa aagagatcca agaggccaag aaacacattc
1021 ctcagctgca agagcagtta tccaaagcca caggctctgc tcaggatgga gctgtagtta
1081 caccctccag acctttaggg gacaccctaa cagtcagtga ggcagaaaca gatcctggag
1141 atgtactggg caggactgac tgtagcagtg gagatgcttc tcggcccagt agtgacaatg
1201 cagatagtcc caagagtggc ccaaaagaga gaatttatct agaggaaaac ccagagaaaa
1261 gtgaaacaat tcaggacact gacactcaat cacttgtcgg aagtccctca acccgtatag
1321 cacctcatat tattggagca gaagatgatg attttggtac tgaacatgaa cagatcaatg
1381 gacagtgcag ctgtttccag agcattgaat tactaaaatc tcgcccggct catttggctg
1441 ttttcttaca ccatgtagtt tcacaatttg accctgcgac tttgctctgt tatctctatt
1501 cagacctgta taaacatacc aattccaaag aaactcgtcg catcttcctt gagtttcatc
1561 agttctttct agatcgatca gcacacctga aagtttctgt tcctgatgaa atgtctgcag
1621 atctagaaaa gagaagacct gagctcattc ctgaggatct gcatcgccac tatatccaaa
1681 ctatgcaaga aagagtccat ccagaagttc aaaggcactt agaagatttt cggcagaaac
1741 gtagtatggg actgaccttg gctgaaagcg agctgactaa acttgatgca gagcgagaca
1801 aggaccgatt gactttggag aaggagcgga catgtgcaga acagattgtt gccaaaattg
1861 aagaagtatt gatgactgct caggctgtag aggaagataa gagctccacc atgcagtatg
1921 ttattctcat gtatatgaag catttgggag taaaagtgaa agagcctcga aatttggagc
1981 acaaacgggg tcggattgga tttcttccca aaatcaagca aagtatgaaa aaaaaaaaaa
2041 aaaaaaaaaa aaaaaaa
//