LOCUS BC063044 2603 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens NIMA (never in mitosis gene a)-related kinase 4, mRNA
(cDNA clone MGC:65120 IMAGE:5169184), complete cds.
ACCESSION BC063044
VERSION BC063044.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2603)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2603)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 119 Row: c Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4507276.
FEATURES Location/Qualifiers
source 1..2603
/db_xref="H-InvDB:HIT000260751"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:65120 IMAGE:5169184"
/tissue_type="Brain, adult medulla"
/clone_lib="NIH_MGC_119"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2603
/gene="NEK4"
/gene_synonym="NRK2"
/gene_synonym="STK2"
/db_xref="GeneID:6787"
/db_xref="MIM:601959"
CDS 194..2539
/gene="NEK4"
/gene_synonym="NRK2"
/gene_synonym="STK2"
/codon_start=1
/product="NEK4 protein"
/protein_id="AAH63044.1"
/db_xref="GeneID:6787"
/db_xref="MIM:601959"
/translation="MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASS
RERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQL
LPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHC
DMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRI
IEGKLPAMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKT
SKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRA
SGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLD
ASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKE
QKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVR
RDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRP
LSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQER
KQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVP
VANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDV
IRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYRIF"
misc_feature 209..976
/gene="NEK4"
/gene_synonym="NRK2"
/gene_synonym="STK2"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain. Phosphotransferases of the serine or
threonine-specific kinase subfamily. The enzymatic
activity of these protein kinases is controlled by
phosphorylation of specific residues in the activation
segment of the catalytic domain, sometimes combined with
reversible conformational changes in the C-terminal
autoregulatory tail"
/db_xref="CDD:cd00180"
BASE COUNT 782 a 601 c 649 g 571 t
ORIGIN
1 gaactgctcc cggcccggat cgctatggca gcggcgtcgt cgcgggccgg gccccagcaa
61 tcccgcccgg gcccggctgc ctcaacagcc gcccccactg ccccctctcg ggcatgaacc
121 gagcttcttg ttgccgcccg ctgccctacc cgccgctgcc gccgcatccc gactctgggc
181 cagcgctggg aacatgcccc tggccgccta ctgctacctg cgggtcgtgg gcaaggggag
241 ctatggagag gtgacgcttg tgaagcaccg gcgggacggc aagcagtatg tcatcaaaaa
301 actgaacctc cgaaatgcct ctagccgaga gcggcgagct gctgaacagg aagcccagct
361 cttgtctcag ttgaagcatc ccaacattgt cacctacaag gagtcatggg aaggaggaga
421 tggtctgctc tacattgtca tgggcttctg tgaaggaggt gatttgtacc gaaagctcaa
481 ggagcagaaa gggcagcttc tgccggagaa tcaggtggta gagtggtttg tacagatcgc
541 catggctttg cagtatttac atgaaaaaca catccttcat cgagatctga aaactcaaaa
601 tgtcttccta acaagaacaa acatcatcaa agtaggggac ctaggaattg cccgagtgtt
661 ggagaaccac tgtgacatgg ctagcaccct cattggcaca ccctactaca tgagccctga
721 attgttctca aacaaaccct acaactataa gtctgatgtt tgggctctag gatgctgtgt
781 ctatgaaatg gccaccttga agcatgcttt caatgcaaaa gatatgaatt ctttagttta
841 tcggattatt gaaggaaagc tgccagcaat gccaagagat tacagcccag agctggcaga
901 actgataaga acaatgctga gcaaaaggcc tgaagaaagg ccgtctgtga ggagcatcct
961 gaggcagcct tatataaagc ggcaaatctc cttctttttg gaggccacaa agataaaaac
1021 ctccaaaaat aacattaaaa atggtgactc tcaatccaag ccttttgcta cagtggtttc
1081 tggagaggca gaatcaaatc atgaagtaat ccacccccaa ccactctctt ctgagggctc
1141 ccagacatat ataatgggtg aaggcaaatg tttgtcccag gagaaaccca gggcctctgg
1201 tctcttgaag tcacctgcca gtctgaaagc ccatacctgc aaacaggact tgagcaatac
1261 cacagaacta gccacaatca gtagcgtaaa tattgacatc ttacctgcaa aagggaggga
1321 ttcagtgagt gatggctttg ttcaggagaa tcagccaaga tatttggatg cctctaatga
1381 gttaggaggt atatgcagta tttctcaagt ggaagaggag atgctgcagg acaacactaa
1441 atccagtgcc cagcctgaaa acctgattcc catgtggtcc tctgacattg tcactgggga
1501 aaagaatgaa ccagtgaagc ctctgcagcc cctaatcaaa gaacaaaagc caaaggacca
1561 ggatcaagtt gctggtgaat gtattataga aaaacagggc agaatccacc cagatttaca
1621 gccacacaac tctgggtctg aaccttccct gtctcgacag cgacggcaaa agaggagaga
1681 acagactgag cacagagggg aaaagagaca ggtccgcaga gatctctttg ctttccaaga
1741 gtcgcctcct cgatttttgc cttctcatcc cattgttggg aaagtggatg tcacatcaac
1801 acaaaaagag gctgaaaacc aacgtagagt ggtcactggg tctgtgagca gttcaaggag
1861 cagtgagatg tcatcatcaa aggatcgacc attatcagcc agagagagga ggcgactaaa
1921 gcagtcacag gaagaaatgt cctcttcagg cccttcagtg aggaaagcgt ctctgagtgt
1981 agcagggcca ggaaaacccc aggaagaaga ccagcccttg cctgcccgac ggctctcctc
2041 tgactgcagc gtcactcagg aaaggaaaca gattcattgt ctgtctgagg atgagttaag
2101 ttcttctaca agttcaactg ataagtcaga tggggattac ggggaaggga aaggtcagac
2161 aaatgaaatt aatgccttgg tacaattgat gactcagacc ctgaaactgg attctaaaga
2221 gagctgtgaa gatgtcccgg tagcaaaccc agtgtcagaa ttcaaacttc atcggaaata
2281 tcgggacaca ctgatacttc atgggaaggt tgcagaagag gcagaggaaa tccattttaa
2341 agagctacct tcagctatta tgccaggttc tgaaaagatc aggagactag ttgaagtctt
2401 gagaactgat gtaattcgtg gcctgggagt tcagctttta gagcaggtgt atgatctttt
2461 ggaggaggag gatgaatttg atagagaggt gagtgtctca ttaactgtat caagatgctt
2521 atgttataga attttttaaa caaccttttg aaatataaat cacacataac aattcaaaaa
2581 aaaaaaaaaa aaaaaaaaaa aaa
//