LOCUS BC063008 2920 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens two pore segment channel 2, mRNA (cDNA clone MGC:70581
IMAGE:5214862), complete cds.
ACCESSION BC063008
VERSION BC063008.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2920)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2920)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 135 Row: i Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20589957.
FEATURES Location/Qualifiers
source 1..2920
/db_xref="H-InvDB:HIT000260718"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:70581 IMAGE:5214862"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2920
/gene="TPCN2"
/gene_synonym="TPC2"
/db_xref="GeneID:219931"
/db_xref="HGNC:HGNC:20820"
CDS 53..2311
/gene="TPCN2"
/gene_synonym="TPC2"
/codon_start=1
/product="two pore segment channel 2"
/protein_id="AAH63008.1"
/db_xref="GeneID:219931"
/db_xref="HGNC:HGNC:20820"
/translation="MAEPQAESEPLLGGARGGGGDWPAGLTTYRSIQVGPGAAARWDL
CIDQAVVFIEDAIQYRSINHRVDASSMWLYRRYYSNVCQRTLSFTIFLILFLAFIETP
SSLTSTADVRYRAAPWEPPCGLTESVEVLCLLVFAADLSVKGYLFGWAHFQKNLWLLG
YLVVLVVSLVDWTVSLSLVCHEPLRIRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMAS
VGLLLAIHLCLFTMFGMLLFAGGKQDDGQDRERLTYFQNLPESLTSLLVLLTTANNPD
VMIPAYSKNRAYAIFFIVFTVIGSLFLMNLLTAIIYSQFRGYLMKSLQTSLFRRRLGT
RAAFEVLSSMVGEGGAFPQAVGVKPQNLLQVLQKVQLDSSHKQAMMEKVRSYGSVLLS
AEEFQKLFNELDRSVVKEHPPRPEYQSPFLQSAQFLFGHYYFDYLGNLIALANLVSIC
VFLVLDADVLPAERDDFILGILNCVFIVYYLLEMLLKVFALGLRGYLSYPSNVFDGLL
TVVLLVLEISTLAVYRLPHPGWRPEMVGLLSLWDMTRMLNMLIVFRFLRIIPSMKPMA
VVASTVLGLVQNMRAFGGILVVVYYVFAIIGINLFRGVIVALPGNSSLAPANGSAPCG
SFEQLEYWANNFDDFAAALVTLWNLMVVNNWQVFLDAYRRYSGPWSKIYFVLWWLVSS
VIWVNLFLALILENFLHKWDPRSHLQPLAGTPEATYQMTVELLFRDILEEPEEDELTE
RLSQHPHLWLCR"
BASE COUNT 555 a 814 c 857 g 694 t
ORIGIN
1 cgccttcggg gcttgagctt ctgagggtcg ggtccagcgc gtgggctgct ggatggcgga
61 accccaggcg gagtcggagc ccctgctggg cggggcccgc ggcggtggcg gcgactggcc
121 ggcggggctg accacttacc gcagcatcca agtcggccct ggtgccgcgg ccaggtggga
181 cctctgcatt gatcaggctg tggtcttcat cgaagatgct attcagtacc gctccatcaa
241 ccaccgggtg gatgccagct cgatgtggct ttaccgacgg tattactcga acgtatgcca
301 acggactttg agcttcacca tcttcttgat cctgtttttg gcttttatcg agaccccatc
361 ctcactcacc agcacggcgg acgtgcgcta ccgcgctgcc ccctgggagc cgccctgcgg
421 cctgaccgag agtgtcgagg tgctctgcct gctggtcttt gcggccgacc tctctgtgaa
481 gggttacctg ttcgggtggg cccatttcca gaaaaacctt tggctgctgg gctacctcgt
541 ggtgctggtg gtgtctctgg tggactggac cgtgtccctg agtctcgtgt gtcatgagcc
601 cctgcggatc cgccggcttc tccgtccctt cttcctgctg cagaactcct ctatgatgaa
661 gaagaccttg aaatgcatcc gctggtcgct gccggaaatg gccagcgtcg ggctgctgct
721 ggccatccac ctgtgcctct tcaccatgtt cggaatgctg ctgttcgctg gtgggaagca
781 ggatgatggg caggacaggg agaggctgac ctacttccag aacctgcctg agtctctgac
841 ttccctcctg gtgctgctga ccacggccaa caaccccgat gtgatgattc ctgcgtattc
901 caagaaccgg gcctatgcca tcttcttcat agtcttcact gtgataggaa gcctgtttct
961 gatgaacctg ctgacagcca tcatctacag tcagttccgg ggctacctga tgaaatctct
1021 ccagacctcg ctgtttcgga ggcggctggg aacccgggct gcctttgaag tcctatcctc
1081 catggtgggg gagggaggag ccttccctca ggcagttggg gtgaagcccc agaacttgct
1141 gcaggtgctt cagaaggtcc agctggacag ctcccacaaa caggccatga tggagaaggt
1201 gcgttcctac ggcagtgttc tgctgtcagc tgaggagttt cagaagctct tcaacgagct
1261 tgacagaagt gtggttaaag agcacccgcc gaggcccgag taccagtctc cgtttctgca
1321 gagcgcccag ttcctcttcg gccactacta ctttgactac ctggggaacc tcatcgccct
1381 ggcaaacctg gtgtccattt gcgtgttcct ggtgctggat gcagatgtgc tgcctgctga
1441 gcgtgatgac ttcatcctgg ggattctcaa ctgcgtcttc attgtgtact acctgttgga
1501 gatgctgctc aaggtctttg ccctgggcct gcgagggtac ctgtcctacc ccagcaacgt
1561 gtttgacggg ctcctcaccg ttgtcctgct ggttttggag atctcaactc tggctgtgta
1621 ccgattgcca cacccaggct ggaggccgga gatggtgggc ctgctgtcgc tgtgggacat
1681 gacccgcatg ctgaacatgc tcatcgtgtt ccgcttcctg cgtatcatcc ccagcatgaa
1741 gccgatggcc gtggtggcca gtaccgtcct gggcctggtg cagaacatgc gtgcttttgg
1801 cgggatcctg gtggtggtct actacgtatt tgccatcatt gggatcaact tgtttagagg
1861 cgtcattgtg gctcttcctg gaaacagcag cctggcccct gccaatggct cggcgccctg
1921 tgggagcttc gagcagctgg agtactgggc caacaacttc gatgactttg cggctgccct
1981 ggtcactctg tggaacttga tggtggtgaa caactggcag gtgtttctgg atgcatatcg
2041 gcgctactca ggcccgtggt ccaagatcta ttttgtattg tggtggctgg tgtcgtctgt
2101 catctgggtc aacctgtttc tggccctgat tctggagaac ttccttcaca agtgggaccc
2161 ccgcagccac ctgcagcccc ttgctgggac cccagaggcc acctaccaga tgactgtgga
2221 gctcctgttc agggatattc tggaggagcc cgaggaggat gagctcacag agaggctgag
2281 ccagcacccg cacctgtggc tgtgcaggtg acgtccgggc tgccgtccca gcaggggcgg
2341 caggagagag aggctggcct acacaggtgc ccgtcatgga agaggcggcc atgctgtggc
2401 cagccaggca ggaagagacc tttcctctga cggaccacta agctggggac aggaaccaag
2461 tcctttgcgt gtggcccaac aaccgtctac agaacagctg ctggtgcttc agggaggcgc
2521 cgtgccctcc gctttctttt atagctgctt cagtgagaat tccctcgtcg actccacagg
2581 gacctttcag acaaaaatgc aagaagcagc ggcctcccct gtcccctgca gcttccgtgg
2641 tgcctttgct gccggcagcc cttggggacc acaggcctga ccagggcctg cacaggttaa
2701 ccgtcagact tccggggcat tcaggtgggg atgctggtgg tttgacatgg agagaacctt
2761 gactgtgttt tattatttca tggcttgtat gagtgtgact gggtgtgttt ctttagggtt
2821 ctgattgcca gttattttca tcaataagtc ttgcaaagaa aaaaaaaaaa aaaaaaaaaa
2881 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//