LOCUS BC063007 1733 bp mRNA linear HUM 02-JUL-2005
DEFINITION Homo sapiens AF4/FMR2 family, member 4, mRNA (cDNA clone
IMAGE:5213733), complete cds.
ACCESSION BC063007
VERSION BC063007.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1733)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1733)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 135 Row: i Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7656878
This clone has the following problem: The cds is short compared to
the longest cds in the locus.
FEATURES Location/Qualifiers
source 1..1733
/db_xref="H-InvDB:HIT000260717"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5213733"
/tissue_type="Blood, adult leukocytes"
/clone_lib="NIH_MGC_118"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1733
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/db_xref="GeneID:27125"
/db_xref="MIM:604417"
CDS 260..1321
/gene="AFF4"
/gene_synonym="AF5Q31"
/gene_synonym="MCEF"
/codon_start=1
/product="AFF4 protein"
/protein_id="AAH63007.1"
/db_xref="GeneID:27125"
/db_xref="MIM:604417"
/translation="MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSK
EDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGS
HQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSG
SSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDA
NWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYS
SQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAI
HTPCKTEPSKFPFPTKVSK"
BASE COUNT 524 a 446 c 405 g 358 t
ORIGIN
1 ccgggcccga ccgtcatgga gcggtgcggg gcctacgcct gttccgtgtg aggcgcatcg
61 ctgccgccgc cgccgacgcc gccttcgttt gtcgaggcct ccggaacccc cgccgcctcc
121 cggagcccct cgcccttggc agcctgtcac cgccccctgg gcgggctcga atgcgcgtcc
181 ggtgaaggtg caggcccggc gccgccactg ccgcagccag gagatggttc gggcctagcg
241 gagccgggac tggagcaaca tgaaccgtga agaccggaat gtgctgcgta tgaaagaacg
301 ggaaaggcgg aatcaggaaa ttcagcaggg cgaagacgcc ttcccaccta gctctcctct
361 ctttgcagag ccatacaaag ttactagcaa agaagataag ttatcaagtc gtattcagag
421 tatgcttgga aactacgatg aaatgaagga tttcatagga gacagatcta taccaaagct
481 tgttgcaatt cccaagccta cagtaccacc atcagcagat gaaaaatcta acccaaattt
541 ctttgaacag agacatggag gctctcatca gagtagcaaa tggactccag taggacccgc
601 acccagcact tctcagtctc agaaacggtc ctcaggctta cagagtggac atagtagcca
661 gcggaccagc gcaggtagca gtagtggcac taacagtagt ggtcagaggc acgaccgtga
721 gtcatataac aatagtggga gcagtagccg gaaaaaaggc cagcatggat cagaacactc
781 caaatcacgt tcttccagcc ctggaaaacc ccaggctgtt tcttcattaa actctagtca
841 ttccaggtct catgggaatg atcaccatag caaggaacat caacgctcca aatcacctcg
901 ggaccctgat gcaaactggg attctccttc ccgtgtacct ttttcaagtg ggcagcactc
961 aactcaatct ttcccaccct cattgatgtc aaagtccaat tcaatgttac agaaacccac
1021 tgcctatgtg cggcccatgg acggacagga gtccatggaa ccaaagctgt cctctgagca
1081 ctacagcagc caatcccatg gcaacagcat gactgagctg aagcccagca gcaaagcaca
1141 tctcaccaag ctgaaaatac cttcccaacc actggatgca tcagcttctg gtgatgtgag
1201 ctgtgtggat gaaatcctaa aagagatgac gcattcatgg cctccccctc taacggctat
1261 tcatacacca tgcaaaacag aaccttccaa atttcctttt ccaactaagg taagtaaata
1321 aaatgtatct ttcataatgt aagaaaactc taaatggctt gactaaaatc atatggatta
1381 aaaattgtct tgccattcct attctagtgg gagacagaca gtaaataagt gaataaatag
1441 ataaattcag atagtgacaa ctgttatgaa gataattagc agggtaatgg aactgagagc
1501 atcttggatc aagaggtatt aagaaagctt tgaaggcaat atgcgagaga gatttaaaag
1561 acattaatac agccggacac ggtggctcac tcctgtaatc ccagcacttt ggaaggctga
1621 gccaagagac tctcttgagg ccaggagttt gcgaccagcc tggtcaacat agcaagaccc
1681 tgtctctacc aaaaattgga aggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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